| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We did comprehensive expression analysis of cTECs and mTECs and isolated transcriptional regulators that showed significant expression difference between cTECs and mTECs. We found that mice deficient in a homeobox transcription factor highly detected in cTECs showed thymus hypoplasia and reduced expression of cTEC-genes. We also focused on PITHD1 highly detected in cTECs. PITHD1 has a putative proteasome binding domain, but is functionally unknown gene. We found that PITHD1 bind with proteasomes in the testis but not in the thymus. PITHD1-KO mice showed severe male infertility accompanied with morphological and motile abnormalities of sperm. PITHD1 deficiency reduced proteasome activity in the testis and altered the amount of proteins important for fertilization capability. However, the PITHD1-KO mice showed no detectable defects in the thymus. Collectively, our results identify PITHD1 as a proteasome-interacting protein that plays a nonredundant role in the male reproductive system.
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