| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
It has been reported that Neurotrypsin (NP) plays an important role in the formation of neuromuscular junction and in the synaptic plasticity in nervous system. In this study we elucidated the expression of NP mRNA in the rat dorsal root ganglia (DRG) and dorsal horn (DH). In the rat DRG, 30% of total neuron expressed NP mRNA. And in DH, neuronal cell in laminae I-II expressed NP mRNA but not in deep layer of DH. Peripheral nerve injury dramatically increased the NP mRNA expression in DRG but not in DH. Agrin is specific substrate of NP and the fragment of agrin act as a specific ligand of cell surface tyrosin kinase Muscle specific kinase (MuSK). We found the phosphorylation of MuSK in the activated microglia of the neuropathic pain model rats. These results indicate the possible involvement of NP in neuro-microglia interaction following peripheral nerve injury.
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