Establishment of a method for diagnosing subtypes of Creutzfeldt-Jakob disease
| Project/Area Number |
17K10022
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 克也 長崎大学, 医歯薬学総合研究科(保健学科), 教授 (70398147)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 孤発性CJD / QUIC法 / クロイツフェルト・ヤコブ病 / プリオン高感度検出法 |
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| Outline of Final Research Achievements |
Sporadic Creutzfeldt-Jakob disease (sCJD), a majority of human prion diseases, is classified in different subtypes which contribute to the occurrence of distinct clinical-pathological phenotypes. In this study, we tested whether a modification of the real-time quaking-induced conversion (RT-QUIC) assay, a sensitive diagnostic test for prion disease, could distinguish subtypes of sCJD. We prepared various PrP peptides and used them in the QUIC assay to derive those that show a promoting or inhibiting effect. RT-QUICs were performed with brain homogenates from several types of prion disease patients. As a result, by using several PrP peptide, there were slight differences in the RT-QUIC signaling between specimens. Furthermore, the use of multiple peptides further affected the RT-QUIC signal between specimens.
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| Academic Significance and Societal Importance of the Research Achievements |
急速な神経細胞変性をきたす致死性の疾患、プリオン病。その代表的な病気である孤発性クロイツフェルト・ヤコブ病（CJD）はタイプにより予後の進行状況が異なることから、発症早期にタイプを含めた孤発性CJDを正確に診断することが臨床現場から切望されている。ここでプリオンタイプによりRT-QUIC法でのPrP重要配列が異なることが示唆されたことは、予後診断法の確立において大きな意義があると考える。
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Report
(4 results)
Research Products
(9 results)
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[Presentation] Simultaneous addition of digitonin, heparin and arginine ethyl ester improves in vitro amplification of PrPSc derived from various prion strains.2018
Author(s)
Imamura M, Tabeta N, Matsuura Y, Iwamaru Y, Kitamoto T, Ma J, Mohri S, Murayama Y, Takatsuki H, Mori T, Atarashi R
Organizer
APPS2018
Related Report
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[Presentation] Highly sensitive detection of PrPSc derived from various prion strains by simultaneous addition of digitonin, heparin and arginine ethyl ester to protein misfolding cyclic amplification.2018
Author(s)
Imamura M, Tabeta N, Matsuura Y, Iwamaru Y, Kitamoto T, Ma J, Mohri S, Murayama Y, Takatsuki H, Mori T, Atarashi R
Organizer
Prion 2018
Related Report
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