| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
I found that fibronectin (FN) was abundant on the surface of extracellular vesicles derived from brain microvascular endothelial cells (MVEC-EVs) and that FN on EVs mediated their internalization into oligodendrocyte precursor cells (OPCs) by its binding to heparan sulfate proteoglycan (HSPG) on OPCs. OPC survival and proliferation promoted by EVs were attenuated by blocking the internalization of EVs into OPCs. Moreover, I also found that platelet-derived growth factor-B (PDGF-B) was on the surface of MVEC-EVs and that PDGF-B was tethered to the EV by HSPG. The neutralizing antibody against PDGF-B significantly reduced the promoting activity of MVEC-EVs on OPC proliferation, suggesting that PDGF-B on the surface of MVEC-EV promotes OPC proliferation.
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