| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Human endometrial stromal cells (ESCs) undergo decidualization, which is crucial for embryo implantation and maintenance of pregnancy. We previously reported that a number of genes are up- or down-regulated during decidualization and that epigenetic changes (increase of H3K27ac) occur throughout the genome during decidualization of ESCs. In this study, we aimed to identify a master transcription regulator of decidualization, which is an up-stream transcription factor that regulates genome-wide gene expressions and epigenome status. Based on genome-wide expression data, we focused on C/EBPb as a candidate mater regulator. RNA-seq analysis with C/EBPb knockdown revealed that 53.4% of up- or downregulated genes by decidualization were under the regulation of C/EBPb. ChIP-seq analysis showed that C/EBPb regulates almost all the increase of H3K27ac (99.3 %) during decidualization. Taken together, our study showed that C/EBPb is one of master regulator for decidualization.
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