Profiling microbiota of bronchial fluids in the elderly and its association with oral cavities

• Project/Area Number: 17K12006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Niigata University
• Principal Investigator: Sato Takuichi 新潟大学, 医歯学系, 教授 (10303132)
• Co-Investigator(Kenkyū-buntansha): 鷲尾 純平 東北大学, 歯学研究科, 講師 (20400260) ; 河村 好章 愛知学院大学, 薬学部, 教授 (80262757) ; 星川 康 藤田医科大学, 医学部, 教授 (90333814) ; 真柳 弦 東北大学, 歯学研究科, 講師 (10451600) ; 高橋 一郎 九州大学, 歯学研究院, 教授 (70241643) ; 加藤 一夫 愛知学院大学, 歯学部, 准教授 (60183266)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 歯学 / 細菌 / 分類学 / 微生物 / 微生物学

Outline of Final Research Achievements

The source of the bacteria involved in silent aspiration remains to be completely defined. The purpose of the present study was to obtain reliable evidence on silent aspiration of oral bacteria in elderly patients. Our results suggest that bacteria in bronchial fluids associated with silent aspiration are derived from saliva, and that the bronchial fluids of elderly patients with an impaired swallowing reflex may have a characteristic microbiota. In addition, our results suggest that oral hygiene is important to reduce silent aspiration and to subsequently prevent the occurrence of aspiration pneumonia in elderly patients.

Academic Significance and Societal Importance of the Research Achievements

本研究において、マイクロサンプリングプローブを用いて目的部位以外の細菌汚染に極力配慮し、気管支液試料を採取し、その細菌量および細菌構成を詳細に解析することが出来、手法の確立に大きく貢献した。本研究成果に基づく結論として、特に高齢者の誤嚥性肺炎の発症の予防のためにも、口腔衛生管理がより一層重要であることが示唆された。

Research Products

• [Int'l Joint Research] Eastman Dental Institute(英国)
• [Int'l Joint Research] Forsyth Institute(米国)
• [Int'l Joint Research] Eastman Dental Institute(英国)
• [Journal Article] Profiling system of oral microbiota utilizing polymerase chain reaction-restriction fragment length polymorphism analysis (2021)
  • Author(s): Sano H, Wakui A, Kawachi M, Washio J, Abiko Y, Mayanagi G, Yamaki K, Tanaka K, Takahashi N, Sato T
  • Journal Title: Journal of Oral Biosciences Volume: 63 Issue: 3 Pages: 292-297
  • DOI: 10.1016/j.job.2021.05.003
  • Peer Reviewed / Open Access
• [Journal Article] Bacterial amounts and compositions in liquid baby formula and a baby drink consumed with an artificial nipple. (2021)
  • Author(s): Wakui A, Sano H, Kawachi M, Aida A, Takenaka Y, Yonezawa A, Nakahata N, Moriyama S, Nishikata M, Washio J, Abiko Y, Mayanagi G, Yamaki K, Sakashita R, Tanaka K, Takahashi N, Sato T
  • Journal Title: J Oral Biosci. Volume: 印刷中 Issue: 2 Pages: 161-168
  • DOI: 10.1016/j.job.2021.03.001
  • Peer Reviewed / Open Access
• [Journal Article] Profiling of microbiota at the mouth of bottles and in remaining tea after drinking directly from plastic bottles of tea (2021)
  • Author(s): Wakui A*, Sano H*, Hirabuki Y*, Kawachi M, Aida A, Washio J, Abiko Y, Mayanagi G, Yamaki K, Tanaka K, Takahashi N, Sato T
  • Journal Title: Dentistry Journal Volume: 9 Issue: 6 Pages: 58-58
  • DOI: 10.3390/dj9060058
  • Peer Reviewed / Open Access
• [Journal Article] A longitudinal study on the relationship of oral health at 4 years of age with that in adulthood (2021)
  • Author(s): Yamada S, Sakashita R, Ogura M, Nakanishi E, Sato T
  • Journal Title: Dent J Volume: 9 Issue: 2 Pages: 17-17
  • DOI: 10.3390/dj9020017
  • Peer Reviewed / Open Access
• [Journal Article] Effects of dining-focused life enhancement program in welfare facilities for seniors in Japan (2020)
  • Author(s): Sakashita R, Ono H, Sato T, Takami M, Kim W, Nakanishi E, Kusumoto H, Hamasaki M, Hamada M:
  • Journal Title: Asian/Pacific Island Nursing Journal Volume: 5 Pages: 63-72
  • Peer Reviewed
• [Journal Article] Nitrite-producing oral microbiome in adults and children (2020)
  • Author(s): Sato-Suzuki Y, Washio J, Wicaksono DP, Sato T, Fukumoto S, Takahashi N
  • Journal Title: Sci Rep Volume: 10 Issue: 1 Pages: 16652-16652
  • DOI: 10.1038/s41598-020-73479-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Responses of oral-microflora-exposed dental pulp to capping with a triple antibiotic paste or calcium hydroxide cement in mouse molars (2020)
  • Author(s): Quispe-Salcedo A, Sato T, Matsuyama J, Ida-Yonemochi H, Ohshima H
  • Journal Title: Regen Ther Volume: 15 Pages: 216-225
  • DOI: 10.1016/j.reth.2020.10.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Impact of the consistency of food substances on health and related factors of residents in welfare facilities for seniors in Japan (2020)
  • Author(s): Sakashita R, Sato T, Ono H, Hamaue A, Hamada M
  • Journal Title: Dentistry Journal Volume: 8 Issue: 1 Pages: 9-9
  • DOI: 10.3390/dj8010009
  • Peer Reviewed / Open Access
• [Journal Article] <b>Exhaled acetone and isoprene in perioperative lung cancer patients under intensive oral care: possible indicators of inflammatory responses and metabolic </b><b>changes </b> (2019)
  • Author(s): 10.1016/j.jdent.2018.12.017
  • Journal Title: Biomedical Research Volume: 40 Issue: 1 Pages: 29-36
  • DOI: 10.2220/biomedres.40.29
  • NAID: 130007603165
  • ISSN: 0388-6107, 1880-313X
  • Year and Date: 2019-02-01
  • Peer Reviewed / Open Access
• [Journal Article] Consideration to health care and gender in Sri Lanka (2019)
  • Author(s): Gunarathne TGNS, Nakamura M, Aoki H, Taguchi M, Hotta K, Ishida M, Yamazaki Y, Sakai S, Sakagami M, Sato M, Sato T, Yamamoto H, Iwafuchi M
  • Journal Title: Journal of Health Sciences of Niigata University Volume: 16 Pages: 1-10
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Draft genome sequences of two Veillonella tobetsuensis clinical isolates from intraoperative bronchial fluids of elderly patients with pulmonary carcinoma (2019)
  • Author(s): Mashima I, Miyoshi-Akiyama T, Tomida-Nakashima J, Kutsuna R, Washio J, Takahashi N, Nakazawa F, Sato T, Kawamura Y
  • Journal Title: Microbiology Resource Announcements Volume: 8 Issue: 38
  • DOI: 10.1128/mra.00397-19
  • Peer Reviewed / Open Access
• [Journal Article] Profiling of microbiota in liquid baby formula consumed with an artificial nipple (2019)
  • Author(s): SANO Hiroto、WAKUI Anna、KAWACHI Miho、KATO Rito、MORIYAMA Sachie、NISHIKATA Mayumi、WASHIO Jumpei、ABIKO Yuki、MAYANAGI Gen、YAMAKI Keiko、SAKASHITA Reiko、TOMIDA Junko、KAWAMURA Yoshiaki、TANAKA Kaori、TAKAHASHI Nobuhiro、SATO Takuichi
  • Journal Title: Biomedical Research Volume: 40 Issue: 4 Pages: 163-168
  • DOI: 10.2220/biomedres.40.163
  • NAID: 130007689885
  • Peer Reviewed / Open Access
• [Journal Article] Consideration to health care and gender in Sri Lanka (2019)
  • Author(s): Gunarathne TGNS, Nakamura M, Aoki H, Taguchi M, Hotta K, Ishida M, Yamazaki Y, Sakai S, Sakagami M, Sato M, Sato T, Yamamoto H, Iwafuchi M
  • Journal Title: J Health Sci Niigata Univ Volume: 16 Pages: 1-10
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Oral health promotion program for fostering self-management of the elderly living in communities (2017)
  • Author(s): Sakashita R, Hamada M, Sato T, Abiko Y, Takami M
  • Journal Title: Intell Autom Soft Comput Volume: 23 Issue: 3 Pages: 535-541
  • DOI: 10.1080/10798587.2017.1348459
  • Peer Reviewed
• [Presentation] Profiling of microbiota in liquid baby formula and a baby drink consumed with an artificial nipple (2022)
  • Author(s): Wakui A, Kawachi M, Nishikata M, Washio J, Abiko Y, Mayanagi G, Takahashi N, Sato T
  • Organizer: International Symposium for IOHS (Interface Oral Health Science) 2022
  • Int'l Joint Research
• [Presentation] Profiling of microbiota in sport-drink and orange juice after drinking directly from plastic bottles (2022)
  • Author(s): Kawachi M, Wakui A, Washio J, Abiko Y, Mayanagi G, Takahashi N, Sato T
  • Organizer: International Symposium for IOHS (Interface Oral Health Science) 2022
  • Int'l Joint Research
• [Presentation] Profiling of microbiota in various drinks after drinking directly from plastic bottles (2021)
  • Author(s): Kawachi M, Takahashi N, Kaku N, Higuchi M, Wakui A, Washio J, Takahashi N, Sato T
  • Organizer: The 63rd Annual Meeting of Japanese Association for Oral Biology
• [Presentation] The composition and structure of oral microbiome: 16S rRNA gene analysis on saliva, plaque, infected root canals obtained from a patient (2021)
  • Author(s): Yamaki K, Washio J, Tamahara T, Sato T
  • Organizer: The 69th Annual Meeting of Japanese Association for Dental Research
  • Int'l Joint Research
• [Presentation] 飲みかけのペットボトル飲料物（お茶、スポーツ飲料、コーラ、オレンジジュース）および飲み口から検出された細菌の量・構成の解析（第3報） (2021)
  • Author(s): 河内美帆，丸山伸吾，枡田菜々子，涌井杏奈，佐野拓人，鷲尾純平，安彦友希，石黒和子，真柳 弦，高橋信博，佐藤拓一
  • Organizer: 第10回口腔保健用機能性食品研究会
• [Presentation] Profiling of microbiota of baby-drinks after drinking with artificial nipples (2020)
  • Author(s): Wakui A, Sano H, Kawachi M, Maruyama S, Masuda N, Washio J, Abiko Y, Mayanagi G, Yamaki K, Takahashi N, Okada Y, Sato T
  • Organizer: The 62nd Annual Meeting of Japanese Association for Oral Biology
  • Invited
• [Presentation] Oral microbiota analyzing system: Profiling by PCR-RFLP method (2020)
  • Author(s): Kawachi M, Maruyama S, Masuda N, Sano H, Wakui A, Yamaki K, Washio J, Takahashi N, Sato T
  • Organizer: The 62nd Annual Meeting of Japanese Association for Oral Biology
• [Presentation] PCR-RFLP法を用いた口腔内細菌叢解析（プロファイリング）システム (2020)
  • Author(s): 涌井杏奈，佐野拓人，河内美帆，丸山伸吾，枡田菜々子，佐藤拓一
  • Organizer: 第60回日本臨床化学会年次学術集会
  • Invited
• [Presentation] 飲みかけのペットボトル飲料物および飲み口から検出された細菌の同定 (2020)
  • Author(s): 河内美帆，丸山伸吾，枡田菜々子，佐野拓人，涌井杏奈，佐藤拓一
  • Organizer: 第73回日本細菌学会関西支部総会
• [Presentation] 母乳および新生児の口腔内の細菌叢解析：保管による影響について (2020)
  • Author(s): 涌井杏奈，佐野拓人，河内美帆，森山幸枝，西方真弓，鷲尾純平，安彦友希，真柳 弦，高橋信博，佐藤拓一
  • Organizer: 第9回口腔保健用機能性食品研究会
• [Presentation] ニプル（哺乳瓶乳首）を通した，口腔から液体ミルク内への流入 (2020)
  • Author(s): 涌井杏奈，佐野拓人，河内美帆，鷲尾純平，安彦友希，真柳 弦，高橋信博，佐藤拓一
  • Organizer: 第9回口腔保健用機能性食品研究会
• [Presentation] Effectiveness of 3Mix-MP paste as pulp-capping agent in murine molars (2020)
  • Author(s): Quispe-Salcedo A, Ohshima H, Sato T
  • Organizer: The 98th General Session of the International Association for Dental Research
  • Int'l Joint Research
• [Presentation] PCR-RFLP法を用いた新しい口腔内細菌叢解析（プロファイル）システム (2019)
  • Author(s): 佐野拓人，涌井杏奈，河内美帆，加藤理都，佐藤拓一
  • Organizer: 第6回U-goサロン
• [Presentation] ニプルを通した，液体ミルク内への口腔細菌の流入 (2019)
  • Author(s): 涌井杏奈，佐野拓人，河内美帆，加藤理都，森山幸枝，西方真弓，佐藤拓一
  • Organizer: 第6回U-goサロン
• [Presentation] Effectiveness of a triple antibiotic paste or calcium hydroxide cement as dental pulp capping treatment on mice molars (2019)
  • Author(s): Quispe-Salcedo A, Sato T, Matsuyama J, Ohshima H
  • Organizer: 第43回 峠の会 形態科学セミナー
• [Presentation] PCR-RFLP法を用いた新しい口腔内細菌叢検査システム (2019)
  • Author(s): 佐野拓人，涌井杏奈，河内美帆，加藤理都，佐藤拓一
  • Organizer: 第14回日本臨床検査学教育学会学術大会
• [Presentation] ニプル（哺乳瓶乳首）を通した，口腔から液体ミルク内への流入 (2019)
  • Author(s): 涌井杏奈，佐野拓人，河内美帆，加藤理都，佐藤拓一
  • Organizer: 第14回日本臨床検査学教育学会学術大会
• [Presentation] 母乳中の細菌叢解析：授乳による影響について (2019)
  • Author(s): 佐野拓人、涌井杏奈、河内美帆、高橋彩朱伽，加藤理都，佐藤拓一
  • Organizer: 第59回日本臨床化学会年次学術集会・学生シンポジウム
  • Invited
• [Presentation] 口腔から乳児用飲料物内への流入：ニプルを通して (2019)
  • Author(s): 涌井杏奈，佐野拓人，河内美帆，高橋彩朱伽，加藤理都，佐藤拓一
  • Organizer: 第59回日本臨床化学会年次学術集会・学生シンポジウム
  • Invited
• [Presentation] Profiling of microbiota in baby-drinks and liquid baby formula consumed with an artificial nipple (2019)
  • Author(s): Wakui A, Sano H, Kawachi M, Washio J, Abiko Y, Mayanagi G, Yamaki K, Takahashi N, Sato T
  • Organizer: The Update Symposium 2 in the 61st Annual Meeting of Japanese Association for Oral Biology
  • Invited
• [Presentation] Profiling system of oral microbiota using PCR-RFLP method (2019)
  • Author(s): Sano H, Wakui A, Washio J, Yamaki K, Takahashi N, Sato T
  • Organizer: The 61st Annual Meeting of Japanese Association for Oral Biology
• [Presentation] Profiling of microbiota of liquid baby formula after drinking with artificial nipples (2019)
  • Author(s): Wakui A, Sano H, Kawachi M, Moriyama S, Nishikata M, Washio J, Yamaki K, Sakashita R, Takahashi N, Sato T
  • Organizer: The 4th Asia Pacific Regional Congress of the International Association for Dental Research
  • Int'l Joint Research
• [Presentation] Profiling system of oral microbiota utilizing PCR-RFLP analysis (2019)
  • Author(s): Sano H, Wakui A, Washio J, Yamaki K, Takahashi N, Sato T
  • Organizer: The 4th Asia Pacific Regional Congress of the International Association for Dental Research
  • Int'l Joint Research
• [Presentation] A novel method to screen and isolate nitrite-producing bacteria from the oral cavity (2019)
  • Author(s): Washio J, Suzuki Y, Sato T, Akiba M, Igarashi A, Kimura S, Tezuka S, Nakazato K, Takahashi N
  • Organizer: International symposium for Interface Oral Health Science (Fuzhou, China)
  • Int'l Joint Research
• [Presentation] 哺乳瓶用乳首（ニプル）を通して乳児用飲料を飲んだ際の，口腔からの逆流について (2019)
  • Author(s): 涌井杏奈，佐野拓人，河内美帆，高橋彩朱伽，加藤理都，鷲尾純平，安彦友希，石黒和子，真柳 弦，高橋信博，佐藤拓一
  • Organizer: 第8回口腔保健用機能性食品研究会（新潟）
• [Presentation] 口腔からベビー飲料内への逆流：ニプル（人工乳首）を通して (2019)
  • Author(s): 河内美帆，高橋彩朱伽，佐藤拓一
  • Organizer: 第8回サイエンス・インカレ（東京）
• [Presentation] 飲み残しのペットボトル飲料物内で増殖する細菌 (2018)
  • Author(s): 佐野拓人，涌井杏奈，曾田彩花，竹中佑太，米澤茜音，中畑那奈，相原 瞳，河内美帆，佐藤拓一
  • Organizer: 第4回U-goサロン（新潟）
• [Presentation] Profiling of microbiota at the mouth of plastic bottles (2018)
  • Author(s): Sano H, Wakui A, Aida A, Takenaka Y, Yonezawa A, Nakahata N, Aihara H, Kawachi M, Washio J, Abiko Y, Mayanagi G, Ishiguro K, Yamaki K, Takahashi N, Sato T
  • Organizer: The 96th General Session of International Association for Dental Reaearch (London)
  • Int'l Joint Research
• [Presentation] ニプルを通して飲んだ際の，口腔から乳児用飲料内への逆流：細菌学的解析 (2018)
  • Author(s): 竹中佑太，涌井杏奈，佐野拓人，曾田彩花，米澤茜音，中畑那奈，相原瞳，河内美帆，佐藤拓一
  • Organizer: 第13回日本臨床検査学教育学会学術大会（札幌）
• [Presentation] Profiling of microbiota in plastic bottles after drinking straight from bottles (2018)
  • Author(s): Sano H, Wakui A, Aida A, Takenaka Y, Yonezawa A, Nakahata N, Aihara H, Kawachi M, Vidanapathirana GU, Washio J, Abiko Y, Mayanagi G, Ishiguro K, Yamaki K, Takahashi N, Sato T
  • Organizer: The 6th Conference on Sri Lanka - Japan Collaborative Research (SLJCR) (Kandy, Sri Lanka)
  • Int'l Joint Research
• [Presentation] Inflow of oral bacteria into drinks for babies through artificial nipples (2018)
  • Author(s): Aihara H, Wakui A, Sano H, Kawachi M, Washio J, Abiko Y, Mayanagi G, Yamaki K, Takahashi N, Sato T
  • Organizer: The 60th Annual Meeting of Japanese Association for Oral Biology (Fukuoka)
• [Presentation] Oral anaerobes in saliva and intraoperative bronchial fluids of elderly patients with pulmonary carcinoma (2018)
  • Author(s): Wakui A, Sano H, Washio J, Yamaki K, Tomida J, Tanaka K, Kawamura Y, Sato T
  • Organizer: The Inaugural Forsyth Symposium - The Uncultivable Bacteria (Cambridge, MA, USA)
  • Int'l Joint Research
• [Presentation] ベビー飲料内への口腔細菌の流入：その詳細な細菌構成 (2018)
  • Author(s): 米澤茜音，佐野拓人，涌井杏奈，中畑那奈，相原 瞳，河内美帆，佐藤拓一
  • Organizer: 第55回日本細菌学会中部支部総会（金沢）
• [Presentation] 哺乳瓶用乳首を通して乳児用飲料を飲んだ際の口腔内細菌の流入について (2018)
  • Author(s): 松山順子，河野承子，三富智恵，朝日藤富子，佐藤拓一
  • Organizer: 第37回日本小児歯科学会中部地方会大会および総会（静岡）
• [Presentation] Profiling of microbiota of baby-drinks after drinking with artificial nipples (2018)
  • Author(s): Wakui A, Sano H, Aihara H, Kawachi M, Takahashi A, Washio J, Abiko Y, Mayanagi G, Ishiguro K, Yamaki K, Takahashi N, Sato T
  • Organizer: The 66th Annual Meeting of Japanese Association for Dental Research (Sapporo)
  • Int'l Joint Research
• [Presentation] ペットボトルの口の部分に付着する細菌および飲料物中の細菌の量および構成の解析（第2報） (2018)
  • Author(s): 河内美帆，佐野拓人，涌井杏奈，平吹有香，曾田彩花，竹中佑太，米田茜音，中畑那奈，相原瞳，Vidanapathirana GU，鷲尾純平，安彦友希，石黒和子，真柳 弦，高橋信博，佐藤拓一
  • Organizer: 第7回口腔保健用機能性食品研究会
• [Presentation] Microbiota profiling at the mouth of plastic bottles after drinking straight from bottles (2018)
  • Author(s): Sano H, Aida A, Vidanapathirana GU, Wakui A, Hirabuki Y, Takenaka Y, Kawachi M, Aihara H, Washio J, Abiko Y, Mayanagi G, Ishiguro K, Yamaki K, Takahashi N, Sato T
  • Organizer: International Symposium for Multimodal Research and Education in IOHS-Liaison 2018
  • Int'l Joint Research
• [Presentation] A microbiological study on bacteria in the PET bottles after drinking (2017)
  • Author(s): Aida A, Sano H, Wakui A, Hirabuki Y, Takenaka Y, Kawachi M, Vidanapathirana GU, Washio J, Abiko Y, Mayanagi G, Ishiguro K, Yamaki K, Takahashi N, Sato T
  • Organizer: 第65回国際歯科研究学会日本部会（JADR）総会・学術大会
  • Int'l Joint Research
• [Presentation] 液体ミルクの安全性の立証に向けて：細菌学的解析 (2017)
  • Author(s): 佐野拓人，涌井杏奈，平吹有香，曾田彩花，竹中佑太，河内美帆，佐藤拓一
  • Organizer: 新大産学交流フェスタ2017
• [Presentation] Development of a life enhancement program focusing on dining for senior home care patients: A pilot study. (2017)
  • Author(s): Sakashita R, Hamada M, Izumoto M, Ono K, Takami M, Ono H, Sato T
  • Organizer: International Nursing Research Conference
  • Int'l Joint Research
• [Presentation] ペットボトル飲料物の飲み口に付着する細菌の検出および同定 (2017)
  • Author(s): 平吹有香，涌井杏奈，佐野拓人，曾田彩花，竹中佑太，河内美帆，佐藤拓一
  • Organizer: 第54回日本細菌学会中部支部会
• [Presentation] 液体ミルクの安全性の立証に向けて：飲料物の飲み口に付着する細菌の量および構成の解析 (2017)
  • Author(s): 涌井杏奈，平吹有香，佐野拓人，曾田彩花，佐藤拓一
  • Organizer: 第57回日本臨床化学会年次学術集会
• [Presentation] Quantification and composition of remaining bacteria in plastic bottles after drinking (2017)
  • Author(s): Sano H, Hirabuki Y, Wakui A, Aida A, Abiko Y, Yamaki K, Mayanagi G, Washio J, Takahashi N, Sato T
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] 液体ミルクの安全性の立証に向けて：飲料物の飲み口に付着する細菌の検出および同定 (2017)
  • Author(s): 涌井杏奈，佐野拓人，平吹有香，曾田彩花，佐藤拓一
  • Organizer: 第12回日本臨床検査学教育学会学術大会
• [Book] 歯科衛生学用語辞典 (2019)
  • Author(s): 全国歯科衛生士教育協議会（監修）、高橋信博、鷲尾純平、真柳弦、安彦友希、佐藤拓一、山本秀輝、涌井杏奈、佐野拓人ほか（著）
  • Total Pages: 480
  • Publisher: 永末書店
  • ISBN: 4816013687
• [Book] 歯科衛生学用語辞典 (2019)
  • Author(s): 高橋信博，佐藤拓一，山本秀輝，佐野拓人，涌井杏奈，他；（監修）全国歯科衛生士教育協議会
  • Publisher: 永末書店