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Deciphering developmental signal intertwined with primary metabolism by trans-omics approach

Research Project

Project/Area Number 17K15147
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionNational Institute for Basic Biology (2019)
Center for Novel Science Initatives, National Institutes of Natural Sciences (2018)
Okazaki Research Facilities, National Institutes of Natural Sciences (2017)

Principal Investigator

Kawade Kensuke  基礎生物学研究所, 共生システム研究部門, 助教 (90612086)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsシロイヌナズナ / ヒメツリガネゴケ / ANGUSTIFOLIA3 / アミノ酸代謝 / 茎葉体 / トランスオミクス / シュート形成 / アルギニン / 細胞増殖 / トランスオミクス解析 / レドックス状態 / 一次代謝 / シグナル伝達 / 低酸素応答 / 発生・分化 / 発現制御
Outline of Final Research Achievements

Pattern formation of organisms requires cell proliferation and expansion, indicating that there are mechanisms, which connect tissue patterning with metabolic reprogramming to stimulate these cellular processes. However, it is unclear how metabolism is modified in response to cell proliferation and expansion. We here addressed this question using plant seedlings as a model. One important finding is an identification of a mechanism for cell proliferation mediated by a regulation of amino acid metabolism. Moreover, we clarified that the integration between tissue patterning and amino acid metabolism has been already established in an early land plant. Interestingly, in the early land plant, this mechanism may facilitate complex tissue patterning including shoot formation because we observed stunted and simple shoot morphology when disrupted. These results thus augmented our understanding in mechanisms by which developmental and metabolic processes are integrated for tissue patterning.

Academic Significance and Societal Importance of the Research Achievements

本研究により、植物の形づくりに関して代謝生理的な視点から重要な知見が得られた。このような発生と代謝のつながりを強く意識した研究は未開拓な領域であり、新しい学術領域を展開する基盤となることが大いに期待できる。また本成果は、作物における有用形質の改良を目指した育種戦略の構築や、フィールドにおける栽培戦略の予測的展開についても貢献しうるものである。より地球規模で考えた場合、深刻化が進んでいる環境問題を解決するバイオマス増産などの指針についても関係する成果であると言える。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (31 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (19 results) (of which Int'l Joint Research: 1 results,  Invited: 7 results) Remarks (4 results)

  • [Int'l Joint Research] ジャック・モノー研究所(フランス)

    • Related Report
      2017 Research-status Report
  • [Journal Article] 代謝システムからみる植物の形づくり2019

    • Author(s)
      川出 健介
    • Journal Title

      アグリバイオ

      Volume: 3 Pages: 49-53

    • NAID

      40021926488

    • Related Report
      2019 Annual Research Report
  • [Journal Article] 表皮細胞の大きさと核内倍加のサイコロゲーム2019

    • Author(s)
      川出 健介
    • Journal Title

      BSJ-Review

      Volume: 10 Issue: A Pages: 14-21

    • DOI

      10.24480/bsj-review.10a3.00150

    • ISSN
      2432-9819
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The cytochrome P450 CYP77A4 is involved in auxin-mediated patterning of the Arabidopsis thaliana embryo2018

    • Author(s)
      Kawade Kensuke、Li Yimeng、Koga Hiroyuki、Sawada Yuji、Okamoto Mami、Kuwahara Ayuko、Tsukaya Hirokazu、Hirai Yokota Masami
    • Journal Title

      Development

      Volume: 145 Issue: 17 Pages: 168369-168369

    • DOI

      10.1242/dev.168369

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pyrophosphate inhibits gluconeogenesis by restricting UDP-glucose formation in vivo2018

    • Author(s)
      Ferjani Ali、Kawade Kensuke、Asaoka Mariko、Oikawa Akira、Okada Takashi、Mochizuki Atsushi、Maeshima Masayoshi、Hirai Masami Yokota、Saito Kazuki、Tsukaya Hirokazu
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 14696-14696

    • DOI

      10.1038/s41598-018-32894-1

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel method for single-grain-based metabolic profiling of Arabidopsis seed2017

    • Author(s)
      Sawada Yuji、Tsukaya Hirokazu、Li Yimeng、Sato Muneo、Kawade Kensuke、Hirai Masami Yokota
    • Journal Title

      Metabolomics

      Volume: 13 Issue: 6 Pages: 75-75

    • DOI

      10.1007/s11306-017-1211-1

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spatially Different Tissue-Scale Diffusivity Shapes ANGUSTIFOLIA3 Gradient in Growing Leaves2017

    • Author(s)
      Kawade Kensuke、Tanimoto Hirokazu、Horiguchi Gorou、Tsukaya Hirokazu
    • Journal Title

      Biophysical Journal

      Volume: 113 Issue: 5 Pages: 1109-1120

    • DOI

      10.1016/j.bpj.2017.06.072

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Probing the stochastic property of endoreduplication in cell size determination of Arabidopsis thaliana leaf epidermal tissue2017

    • Author(s)
      Kawade Kensuke、Tsukaya Hirokazu
    • Journal Title

      PLoS One

      Volume: 12 Issue: 9 Pages: e0185050-e0185050

    • DOI

      10.1371/journal.pone.0185050

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Transcriptome landscape connecting arginine metabolism with gametophore formation in Physcomitrella patens2020

    • Author(s)
      Kensuke Kawade, Yuu Hirose, Gorou Horiguchi, Akira Oikawa, Masami Yokota Hirai, Kazuki Saito, Tomomichi Fujita, Hirokazu Tsukaya
    • Organizer
      第61回 日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物の幹細胞活性とアルギニン代謝を紐付ける仕組み2019

    • Author(s)
      川出 健介
    • Organizer
      第42回 日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ヒメツリガネゴケ茎葉体の発生とアルギニン代謝2019

    • Author(s)
      川出 健介、堀口 吾朗、及川 彰、平井 優美、斉藤 和季、藤田 知道、塚谷 裕一
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ヒメツリガネゴケ茎葉体の発生とアルギニン代謝2019

    • Author(s)
      川出 健介、堀口 吾朗、及川 彰、平井 優美、斉藤 和季、藤田 知道、塚谷 裕一
    • Organizer
      日本植物形態学会第31回総会・大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] A CYTOCHROME P450 CYP77A4 IS REQUIRED FOR AUXIN-MEDIATED PATTERNING OF THE ARABIDOPSIS THALIANA EMBRY2018

    • Author(s)
      Kawade Kensuke、Li Yimeng、Koga Hiroyuki、Sawada Yuji、Kuwahara Ayuko、Tsukaya Hirokazu、Hirai Yokota Masami
    • Organizer
      29th International Conference on Arabidopsis Research
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] シトクロムP450酵素変異株のフェノーム解析2018

    • Author(s)
      川出 健介, 李 一蒙, 古賀 皓之, 澤田 有司, 岡本 真美, 桑原 亜由子, 塚谷 裕一, 平井 優美
    • Organizer
      日本植物形態学会第30回総会・大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 核内倍加の確率論的なふるまいと表皮細胞のサイズ分布2018

    • Author(s)
      川出 健介
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 発生シグナルの時空間動態を解くための実験と理論2018

    • Author(s)
      川出 健介
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] A cytochrome P450 epoxidase regulates embryonic patterning in Arabidopsis thaliana2018

    • Author(s)
      Kensuke Kawade, Yimeng Li, Yuji Sawada, Hirokazu Tsukaya, Masami Yokota Hirai
    • Organizer
      日本植物生理学会
    • Related Report
      2017 Research-status Report
  • [Presentation] an3-dependent compensation during the leaf development acts cell-autonomously in epidermis and non-cell-autonomously in palisade mesophyll tissue2018

    • Author(s)
      野崎 守、川出 健介、堀口 吾朗、塚谷 裕一
    • Organizer
      日本植物生理学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Quantitative dissection of ABA-mediated suppression of macromolecular transport through plasmodesmata2018

    • Author(s)
      Takumi Tomoi, Munenori Kitagawa, Yoichi Sakata, Kensuke Kawade, Hirokazu Tsukaya, Tomomichi Fujita
    • Organizer
      日本植物生理学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Plant morphogen intertwined with metabolism2017

    • Author(s)
      Kensuke Kawade
    • Organizer
      ConBio2017(2017年度 生命科学系学会合同年次大会)
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 組織内の偏った拡散性が細胞間シグナル因子の発現勾配を形づくる2017

    • Author(s)
      川出 健介
    • Organizer
      日本細胞生物学会大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 植物における「発生シグナル」を介したレドックス調節;トランスオミクスによるアプローチ2017

    • Author(s)
      川出 健介
    • Organizer
      生理学研究所 研究会「オルガネラダイナミクスの新規制御機構とその病態生理」
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Biased distribution of tissue-scale diffusivity shapes ANGUSTIFOLIA3 signaling gradient in growing leaf tissue2017

    • Author(s)
      Kensuke Kawade
    • Organizer
      日本発生生物学会大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 核内倍加の確率論的な特性を組み込んだ細胞サイズ決定モデル2017

    • Author(s)
      川出 健介
    • Organizer
      日本植物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 不定根形成を指標としたシロイヌナズナDMSO高感受性変異体の単離と表現型解析2017

    • Author(s)
      野崎 守、川出 健介、塚谷 裕一、杉山 宗隆
    • Organizer
      日本植物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] AN3 regulates gametophytic growth in Physcomitrella patens2017

    • Author(s)
      Griffin St. Clair, Kensuke Kawade, Gorou Horiguchi, Tomomichi Fujita, Hirokazu Tsukaya
    • Organizer
      日本植物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 核内倍加の確率論的な特性を組み込んだ細胞サイズ決定モデル2017

    • Author(s)
      川出 健介、塚谷 裕一
    • Organizer
      日本植物形態学会
    • Related Report
      2017 Research-status Report
  • [Remarks] 研究室ウェブページ

    • URL

      http://www.oib.orion.ac.jp/metabolo/

    • Related Report
      2019 Annual Research Report
  • [Remarks] ORCID

    • URL

      https://orcid.org/0000-0003-1701-4567

    • Related Report
      2019 Annual Research Report
  • [Remarks] 研究室Webページ

    • URL

      http://www.oib.orion.ac.jp/metabolo/

    • Related Report
      2018 Research-status Report
  • [Remarks] 研究室のwebページ

    • URL

      http://www.oib.orion.ac.jp/metabolo/

    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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