Environmental adaptation of cosmopolitan microorganisms living in deep-sea hydrothermal environments and the application of insights in the microbial genetics to evaluation of the ocean current

• Project/Area Number: 17K15301
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: Hokkaido University
• Principal Investigator: Mino Sayaka 北海道大学, 水産科学研究院, 助教 (00755663)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 深海底熱水活動域 / 生物地理 / Campylobacteria / Epsilonproteobacteria / 微生物群集構造 / 分散 / 微生物地理 / 熱水活動域 / MLSA / 微生物分布 / 集団遺伝学

Outline of Final Research Achievements

The objectives of this study are to understand the environmental adaptation and the distribution pattern of cosmopolitan microorganisms living in hydrothermal environments, and to develop the fundamentals for evaluating the ocean currents from the aspect of biological insights. We have obtained the isolates of cosmopolitan species from the shallow and deep-sea hydrothermal environments, and have compared their phenotypic characteristics. In addition, in order to evaluate their distribution pattern, we have analyzed the genetic diversity of the isolates by MLSA.

Academic Significance and Societal Importance of the Research Achievements

海洋の還元的環境に普遍的に生息するコスモポリタン微生物のを対象に，そのゲノムワイドな遺伝的多様性を評価することは，海洋微生物の分布様式の形成や種分化メカニズムの理解に寄与するとともに，生物学的側面から海洋循環を評価する新規手法構築の足がかりになることが期待できる。さらに，本研究で得た新規性の高い難培養微生物株は，難培養性系統群の生理生態学的特徴の理解にも貢献するものである。

Research Products

• [Journal Article] Hydrogenimonas urashimensis sp. nov., a hydrogen-oxidizing chemolithoautotroph isolated from a deep-sea hydrothermal vent in the Southern Mariana Trough (2021)
  • Author(s): Mino Sayaka、Shiotani Taiki、Nakagawa Satoshi、Takai Ken、Sawabe Tomoo
  • Journal Title: Systematic and Applied Microbiology Volume: 44 Issue: 1 Pages: 126170-126170
  • DOI: 10.1016/j.syapm.2020.126170
  • NAID: 120007187742
  • Peer Reviewed
• [Journal Article] Nitrosophilus alvini gen. nov., sp. nov., a hydrogen-oxidizing chemolithoautotroph isolated from a deep-sea hydrothermal vent in the East Pacific Rise, inferred by a genome-based taxonomy of the phylum “Campylobacterota” (2020)
  • Author(s): Shiotani Taiki、Mino Sayaka、Sato Wakana、Nishikawa Sayo、Yonezawa Masanori、Sievert Stefan M.、Sawabe Tomoo
  • Journal Title: PLOS ONE Volume: 15 Issue: 12 Pages: e0241366-e0241366
  • DOI: 10.1371/journal.pone.0241366
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Biogeochemical Implications of N2O-Reducing Thermophilic Campylobacteria in Deep-Sea Vent Fields, and the Description of Nitratiruptor labii sp. nov. (2020)
  • Author(s): Fukushi Muneyuki、Mino Sayaka、Tanaka Hirohisa、Nakagawa Satoshi、Takai Ken、Sawabe Tomoo
  • Journal Title: iScience Volume: 23 Issue: 9 Pages: 101462-101462
  • DOI: 10.1016/j.isci.2020.101462
  • Peer Reviewed / Open Access
• [Presentation] 沖縄トラフ深海底熱水活動域由来の新規好熱性 Campylobacteria (2020)
  • Author(s): 深澤操，美野さやか，他3名
  • Organizer: 令和2年度日本水産学会北海道支部大会
• [Presentation] Distribution pattern of microbial communities in the shallow-water hydrothermal field off Kueishan Island, Taiwan (2018)
  • Author(s): 冨永千晶、美野さやか
  • Organizer: 日本微生物生態学会 第32回大会
• [Presentation] 沖縄近海の熱水域におけるコスモポリタン微生物の地理的分布様式 (2017)
  • Author(s): 美野さやか
  • Organizer: 第51回ビブリオシンポジウム