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Functional analysis of enhancers to dissect differential roles of BMP7 secreted from distinct sites in kidney.

Research Project

Project/Area Number 17K16072
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Kidney internal medicine
Research InstitutionKyoto University (2019)
Keio University (2017-2018)

Principal Investigator

Tsujimura Taro  京都大学, 高等研究院, 特定講師 (90741893)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsエンハンサー / BMP7 / 腎臓 / CTCF / Bmp7 / シングルセルRNA-seq / クロマチン高次構造 / 腎機能 / 遺伝子 / クロマチン / マウス / ゲノム編集
Outline of Final Research Achievements

Regarding the enhancer regulation for the Bmp7 expression in kidneys, this study shows that i) the downstream region of Bmp7 does not contain enhancers that are critical for the normal kidney development; ii) the Bmp7 expression in the adult kidney is limited to only small population of cells; iii) the CTCF binding sites at the locus for sure critically impinge on the enhancer regulation; iv) however, the role for the CTCF binding sites specifically in the kidney cannot be ensured unless the precise position of the enhancer is identified and the epigenetic states around the region are revealed.

Academic Significance and Societal Importance of the Research Achievements

Bmp7の制御という観点からは、より理解を深めていくために、シングルセルレベルでの詳細な解析が必要になってくることを示唆した。エンハンサー制御に関連して得たCTCFの機能およびその調節動態についての知見は、他の遺伝子座においても同様であるはずで、腎臓のみならず広く生体機能をゲノムから探る上での大きな指針となる研究になったと考えている。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (8 results)

All 2020 2019 2018 2017

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 3 results) Presentation (3 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Controlling gene activation by enhancers through a drug-inducible topological insulator2020

    • Author(s)
      Taro Tsujimura, Osamu Takase, Masahiro Yoshikawa, Etsuko Sano, Matsuhiko Hayashi, Kazuto Hoshi, Tsuyoshi Takato, Atsushi Toyoda, Hideyuki Okano, Keiichi Hishikawa
    • Journal Title

      eLife

      Volume: -

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Controlling gene activation by enhancers through a drug-inducible topological insulator.2019

    • Author(s)
      Tsujimura T, Takase O, Yoshikawa M, Sano E, Hayashi M, Hoshi K, Takato T, Toyoda A, Okano H, Hishikawa K
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/534073

    • Related Report
      2018 Research-status Report
    • Open Access
  • [Journal Article] Control of Directionality of Chromatin Folding for the Inter- and Intra-Domain Contacts at the Tfap2c-Bmp7 Locus2018

    • Author(s)
      Tsujimura T, Takase O, Yoshikawa M, Sano E, Hayashi M, Takato T, Toyoda A, Okano H, Hishikawa K.
    • Journal Title

      Epigenetic & Chromatin

      Volume: 11 Issue: 1 Pages: 51-51

    • DOI

      10.1186/s13072-018-0221-1

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] クロマチン高次構造から考える遺伝子制御の制約と可塑性2018

    • Author(s)
      辻村太郎
    • Organizer
      第20回日本進化学会大会シンポジウム『表現型の可塑性~ホモ・サピエンスの環境適応のヒントを探る』
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] クロマチン高次ドメイン構造分断機構の解明とiPS細胞におけるその応用2018

    • Author(s)
      辻村太郎、高瀬敦、吉川真弘、佐野悦子、高戸毅、林松彦、豊田敦、岡野栄之、菱川慶一
    • Organizer
      第17回日本再生医療学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Tfap2c-Bmp7遺伝子座で互いに反目方向に並ぶ2つのCTCF結合クラスターは、クロマチンの隣接コンタクトドメインを分断し、かつ内部コンタクトパターンに関与する、2017

    • Author(s)
      辻村太郎、高瀬敦、吉川真弘、佐野悦子、高戸毅、豊田敦、岡野栄之、菱川慶一
    • Organizer
      第40回日本分子生物学会年会(ConBio2017)
    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] エンハンサーブロッカー及びその使用2019

    • Inventor(s)
      辻村太郎、岡野栄之、菱川慶一
    • Industrial Property Rights Holder
      慶應義塾大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] エンハンサーブロッカー及びその使用2018

    • Inventor(s)
      辻村太郎、岡野栄之、菱川慶一
    • Industrial Property Rights Holder
      慶應義塾大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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