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Development of enzyme-based biofuel cells using a lytic polysaccharide monooxygenase-based electrode

Research Project

Project/Area Number 17K17703
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Bio-related chemistry
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Takeda Kouta  東京農工大学, 工学(系)研究科(研究院), 特任助教 (20781123)

Research Collaborator Nakamura Nobuhumi  
Igarashi Kiyohiko  
Yoshida Makoto  
Ishida Takuya  
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords酵素電極 / 糖質関連酵素 / PQQ / 結晶構造解析 / 直接電子移動 / ヘム / 木材腐朽菌 / 直接電子移動反応 / バイオ燃料電池 / CDH / 酵素電極反応 / 生物電気化学 / モノオキシゲナーゼ / 酵素バイオ電池 / 木材腐朽
Outline of Final Research Achievements

Wood rotting fungi are important decomposers of plant biomass and secrete not only a set of glycoside hydrolases but also numerous oxidoreductases, including lytic polysaccharide monooxygenases (LPMOs). The new insights into bioelectrocatalysis of fungal oxidoreductases were obtained with the goal of the development of enzyme-based biofuel cells using them. LPMO based on the gold nanoparticles modified electrode exhibited the direct electron transfer. For an anodic enzyme, we successfully determined holo-form of the X-ray structure in the pyranose dehydrogenase from Coprinopsis cinerea (CcPDH), providing direct evidence that PQQ is a coenzyme of this enzyme. Furthermore, we demonstrated the electron transfer reaction and bioelectrocatalysis of CcPDH.

Academic Significance and Societal Importance of the Research Achievements

学術的意義は、LPMOへの電子供与体を電極に置き換えたLPMO-電極共役反応系を確立した点で、本電極系によって電気化学的にLPMOの触媒機構や生理学役割について解析できる可能性が示された。直接電子移動型のアノード触媒酵素として使用できるPQQ依存性脱水素酵素に関して、効率的な酵素電極反応を実現するための重要な知見が得られた。以上の本研究成果は、木材腐朽菌の酸化還元酵素を用いたバイオエレクトロニク分野への展開につながるものである。また、糸状菌の生体機能を利用したセルロース系バイオマス変換技術の発展に寄与できるものと考える。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (25 results)

All 2019 2018 2017 Other

All Int'l Joint Research (3 results) Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (18 results) (of which Int'l Joint Research: 6 results)

  • [Int'l Joint Research] Max Planck Institute/Chemical Energy Conversion(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Norwegian University of Life Sciences(ノルウェー)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Max Planck Institute/Chemical Energy Conversion(ドイツ)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Fungal PQQ-dependent dehydrogenases and their potential in biocatalysis2019

    • Author(s)
      Kouta Takeda、Kiwamu Umezawa、Aniko Varnai、Vincent GH Eijsink、Kiyohiko Igarashi、Makoto Yoshida、Nobuhumi Nakamura
    • Journal Title

      Current Opinion in Chemical Biology

      Volume: 49 Pages: 113-121

    • DOI

      10.1016/j.cbpa.2018.12.001

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 尿によるガンのファーストスクリーニング2018

    • Author(s)
      武田康太、中村暢文
    • Journal Title

      Chemical Sensors

      Volume: 34 Pages: 104-110

    • NAID

      40021715654

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Immobilization of Pyrroloquinoline Quinone-Dependent Alcohol Dehydrogenase with a Polyion Complex and Redox Polymer for a Bioanode2017

    • Author(s)
      Sakurada Yuki、Takeda Kouta、Ohno Hiroyuki、Nakamura Nobuhumi
    • Journal Title

      Catalysts

      Volume: 7 Issue: 10 Pages: 296-296

    • DOI

      10.3390/catal7100296

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enzymes Suitable for Biorefinery to Coproduce Hexaric Acids and Electricity from Hexuronic Acids Derived from Biomass2017

    • Author(s)
      Sakuta Riku、Takeda Kouta、Igarashi Kiyohiko、Ohno Hiroyuki、Nakamura Nobuhumi
    • Journal Title

      Energy Technology

      Volume: 6 Issue: 2 Pages: 273-279

    • DOI

      10.1002/ente.201700404

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 自己組織化単分子膜修飾金電極上におけるPQQ依存性脱水素酵素の電子移動反応2019

    • Author(s)
      南達基、武田康太、松田聖史、吉田誠、五十嵐圭日子、大野弘幸、中村暢文
    • Organizer
      電気化学会第86回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The first eukaryotic PQQ enzayme from the plant saprophytic basidiomycete Coprinopsis cinerea2018

    • Author(s)
      Kouta Takeda
    • Organizer
      International Symposium on Biological Material Science for Agriculture and Engineering
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of a PQQ domain of a pyrroloquinoline quinone dependent pyranose dehydrogenase from Coprinopsis cinerea2018

    • Author(s)
      Tatsuki Minami、Kouta Takeda, Takuya Ishida, Makoto Yoshida, Kiyohiko Igarashi, Hiroyuki Ohno, Nobuhumi Nakamura
    • Organizer
      International Symposium on Biological Material Science for Agriculture and Engineering
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The electrochemical behavior of a catalytic site of PQQ-dependent pyranose dehydrogenase with direct electron transfer2018

    • Author(s)
      Kouta Takeda、Makoto Yoshida、Kiyohiko Igarashi、Masahiro Samejima, Hiroyuki Ohno、Nobuhumi Nakamura
    • Organizer
      69th Annual ISE Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 担子菌由来の細胞外PQQ依存性脱水素酵素のシトクロムドメインの電子移動反応2018

    • Author(s)
      南 達基、武田 康太、吉田 誠、五十嵐 圭日子、大野 弘幸、中村 暢文
    • Organizer
      第12回バイオ関連化学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] バイオ燃料電池をリアクターとして用いるガラクタル酸生産2018

    • Author(s)
      阿部 隼人、作田 陸、武田 康太、五十嵐 圭日子、大野 弘幸、中村 暢文
    • Organizer
      電気化学会秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Investigation of election transfer reaction of PQQ-dependent pyranose dehydrogenase from coprinopsis cinerea and its amino acid substitution mutants on electrodes2018

    • Author(s)
      Tatsuki Minami、Kouta Takeda、Makoto Yoshida、Kiyohiko Igarashi、Hiroyuki Ohno、Nobuhumi Nakamura
    • Organizer
      9th Asian Biological Inorganic Chemistry Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Electrical energy extraction in the process of an enzymatic galactaric acid synthesis2018

    • Author(s)
      Hayato Abe、Riku Sakuta、Kouta Takeda、Kiyohiko Igarashi、Hiroyuki Ohno、Nobuhumi Nakamura
    • Organizer
      9th Asian Biological Inorganic Chemistry Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Amperometric biosensor for cancer screening in urine2018

    • Author(s)
      Kouta Takeda、Ryo Kusuoka、Kiyohiko Igarashi、Hiroyuki Ohno、Nobuhumi Nakamura
    • Organizer
      第28回日本MRS年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] A biofuel cell with galactaric acid synthesis as an anode reaction2018

    • Author(s)
      Hayato Abe、Riku Sakuta、Kouta Takeda、Kiyohiko Igarashi、Hiroyuki Ohno、Nobuhumi Nakamura
    • Organizer
      第28回日本MRS年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Construction of an L-Fucose Biosensor Using a PQQ Domain of PQQ-Dependent Pyranose Dehydrogenase2018

    • Author(s)
      Nohoko Sato、Ryo Kusuoka、Kouta Takeda、Hiroyuki Ohno、Nobuhumi Nakamura
    • Organizer
      第28回日本MRS年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 担子菌由来ピロロキノリンキノン依存性ピラノース脱水素酵素のPQQドメインのキャラクタライズ2018

    • Author(s)
      南 達基、武田 康太、石田 卓也、吉田 誠、五十嵐 圭日子、大野 弘幸、中村 暢文
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Arg interacted heme propionate group important for the electron transfer reaction of fungal cytochrome b domain2017

    • Author(s)
      Kouta Takeda、 Makoto Yoshid、Kiyohiko Igarashi、Masahiro Samejima、Hiroyuki Ohno、Nobuhumi Nakamura
    • Organizer
      14th International Symposium on Applied Bioinorganic Chemistry
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] PQQピラノース脱水素酵素の触媒ドメインの電気化学反応2017

    • Author(s)
      武田 康太、楠岡 諒、吉田 誠、五十嵐 圭日子、鮫島 正浩、Birrell James、Reijerse Edward、Lubitz Wolfgang、大野 弘幸、中村 暢文
    • Organizer
      第11回バイオ関連化学シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 酵素バイオ燃料電池を用いたヘキスロン酸からのヘキサル酸と電気エネルギーの同時生産2017

    • Author(s)
      作田 陸、武田 康太、五十嵐 圭日子、大野 弘幸、中村 暢文
    • Organizer
      2017年電気化学会秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ピロロキノリンキノン依存性アルコール脱水素酵素の固定電極の電気化学的触媒反応2017

    • Author(s)
      櫻田 雄希、武田 康太、大野 弘幸、中村 暢文
    • Organizer
      2017年電気化学会秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 糸状菌由来シトクロムbドメインのヘム近傍のアミノ酸残基への変異導入2017

    • Author(s)
      武田 康太、吉田 誠、五十嵐 圭日子、鮫島 正浩、中村 暢文、大野 弘幸
    • Organizer
      2017年電気化学会秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] レドックスポリマーによるピロロキノリンキノン依存性アルコール脱水素酵素固定電極の作製2017

    • Author(s)
      櫻田雄希、武田康太、大野弘幸、中村暢文
    • Organizer
      第50回酸化反応討論会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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