| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Stuttering is a vocal communication disorder presenting abnormal repetition of vocalization. Here, we adapted songbirds as an animal model to examine the contribution of the basal ganglia nucleus Area X to regulate the repetitive sequence of songs at the cellular level. We induced the specific ablation of GABAergic neurons in bilateral Area X of Bengalese finch (BF) by injecting AAV containing Cre-FLEx system and expressing dtA driven by Dlx promoter. The frequency of syllable repetition significantly increased after injection compared to pre-injection. Such increasing of syllable repetition did not occur in the other BFs that were injected AAV which would express dtA driven by the pan-cellular promoter CMV in Area X. The results suggest that the specific ablation of GABAergic neurons in the basal ganglia may induce excitatory/inhibitory imbalance, which in turn, causes dysregulation of syllable repetitive regulation.
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