| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
A novel optogenetic method was first implemented in 2005. It is becoming a crucial technique in neural science. Microbial rhodopsins are used as optogenetic tools for optical control of electrical activity in target cells. The toolbox has expanded by selection and engineering. This research is to explore the natural world, especially extreme environments, in search of microbial rhodopsins that can serve as novel tools. Although we were unable to obtain promising strains from the high salt concentration environment where was one of our initial targets, we obtained bacterial samples from another extreme environments. Three colored colonies were selected and analyzed the genomes. The nucleotide sequences of two of the strains were successfully determined. The amino acid sequences presumed to be the microbial rhodopsins were different from the previously reported ones but did not show new functions. Isolation methods was improved and can be utilized in our future researches.
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