| Project/Area Number |
17K19631
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Brain sciences and related fields
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2017-06-30 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 光活性化蛋白質 / 記憶・学習 / スパイン / 興奮性シナプス / CREB / CaMKIV / 光プローブ / 行動予想発火 / LOV2ドメイン / シナプス可塑性 / 海馬 / 意思決定 / 光活性化タンパク質 / 場所細胞 |
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| Outline of Final Research Achievements |
We attempted to generate photoactivatable CREB and CaMKIV. Because both are nuclear proteins, we first generated a photoactivatable nuclear localization signal (paNLS). The resultant fusion protein, nuclear export signal-LOV2 domain-nuclear localization signal tagged with mCherry, translocated to the nucleus by photostimualtion. However, when we fused it with CREB and monitored activity by expressing luciferase under CRE, there was a significant leak of activity even under the darkness. We therefore decided to generate a dominant negative CREB and CaMKIV.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、記憶形成のメカニズムに踏み込むもので、多彩な記憶障害を伴う疾患（例えば認知症など）や記憶が病的に更新する疾患（たとえばPTSDや薬物依存）の理解と治療に役立つと期待される。
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