Exploration of pathogenicity caused by association and antigenicity of fibrous protein polymers present on the bacterial cell surface
| Project/Area Number |
17K19751
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Oral Science and related fields
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
川端 重忠 大阪大学, 歯学研究科, 教授 (50273694)
住友 倫子 大阪大学, 歯学研究科, 講師 (50423421)
山口 雅也 大阪大学, 歯学研究科, 助教 (00714536)
鈴木 守 大阪大学, 蛋白質研究所, 准教授 (40280507)
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| Project Period (FY) |
2017-06-30 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | レンサ球菌 / 線毛 / 化膿レンサ球菌 |
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| Outline of Final Research Achievements |
Streptococcus pyogenes produce diverse varieties of pili in a serotype-dependent manner. Structural analysis of the major subunit indicated electrostatic interactions between major subunit molecules. Analysis using mutant strains and recombinant proteins revealed that the electrostatic interaction altered the degree of polymerization of major subunits and affected the ability of Streptococcus pyogenes to adhere to human keratinocytes and to form biofilms. Therefore, it was suggested that the interaction between pilus molecules is involved in virulence.
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| Academic Significance and Societal Importance of the Research Achievements |
グラム陽性細菌の線毛タンパク質の間に生じる静電作用はこれまで報告されてこなかった．近年，化膿レンサ球菌の感染症は社会的に問題になっており，ワクチンの開発が求められている．線毛タンパク質はワクチン抗原の候補とされているため，線毛間相互作用の生物学的意義が明らかになれば，構造と機能を基礎にした抗原設計が可能になる．また，様々な病原性グラム陽性細菌が同様の線毛を産生するため，他菌種においても新規の線毛間相互作用の発見に繋がる可能性が考えられた．
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Report
(3 results)
Research Products
(47 results)
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[Journal Article] Allergic conversion of protective mucosal immunity against nasal bacteria in patients with chronic rhinosinusitis with nasal polyposis.2019
Author(s)
Takeda K, Sakakibara S, Yamashita K, Motooka D, Nakamura S, El Hussien MA, Katayama J, Maeda Y, Nakata M, Hamada S, Standley DM, Hayama M, Shikina T, Inohara H, Kikutani H.
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Journal Title
J Allergy Clin Immunol
Volume: 143
Issue: 3
Pages: 1163-1175
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Streptococcus sanguinis non-coding csRNAs negatively regulate expression of type IV pilus retraction ATPase PilT and biofilm formation2018
Author(s)
Ota C, Morisaki H, Nakata M, Arimoto T, Fukamachi H, Kataoka H, Masuda Y, Suzuki N, Miyazaki T, Okahashi N, Kuwata H
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Journal Title
Infect Immun
Volume: 86
Issue: 3
Pages: 1-13
DOI
Related Report
Peer Reviewed / Open Access
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