| Project/Area Number |
17K19761
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Oral Science and related fields
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| Research Institution | The University of Tokushima |
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Principal Investigator |
Tsunematsu Takaaki 徳島大学, 大学院医歯薬学研究部(医学域), 助教 (70726752)
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| Co-Investigator(Kenkyū-buntansha) |
工藤 保誠 徳島大学, 大学院医歯薬学研究部(歯学域), 准教授 (50314753)
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| Research Collaborator |
ISHIMASU Naozumi
ARAKAKI Rieko
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| Project Period (FY) |
2017-06-30 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 口腔扁平上皮癌 / 転移 / 浸潤 |
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| Outline of Final Research Achievements |
Oral squamous cell carcinoma is major type of tumor arising from oral cavity. It is extremely important factor for poor prognosis. However, previous studies are almost using in vitro experiments but not in vivo experiments. In present study, we tried to establish highly metastatic mice model of oral squamous cells carcinoma by using orthotropic transplantation and shRNA library screening. Finally, we could not reach to perform shRNA library screening but established murine oral squamous cell carcinoma cell lines which can apply for in vivo imaging.
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| Academic Significance and Societal Importance of the Research Achievements |
口腔扁平上皮癌の予後を規定する因子である転移の機構をin vivoで解明することを目的として、口腔扁平上皮癌高転移マウスモデルの作製を試みた。現在までに高転移モデルの樹立まで至らなかったが、in vivoイメージング可能なマウス由来口腔扁平上皮癌細胞株を作製した。本細胞株を用いて今後shRNA libraryスクリーニングを実施し、最終的に口腔扁平上皮癌高転移マウスモデルを作製し、口腔扁平上皮癌の治療や病態解明に役立てることが可能と考えられる。
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