Primary and immortalized cell derived from amami rabbit

Research Project

Project/Area Number	17K20055
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Research Field	Environmental conservation measure and related fields
Research Institution	Iwate University
Principal Investigator	Fukuda Tomokazu 岩手大学, 理工学部, 教授 (40321640)
Co-Investigator(Kenkyū-buntansha)	村山美穂京都大学, 野生動物研究センター, 教授 (60293552)
Project Period (FY)	2017-06-30 – 2023-03-31
Project Status	Completed (Fiscal Year 2022)
Budget Amount *help	¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000) Fiscal Year 2019: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000) Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Keywords	生物多様性 / 絶滅危惧種 / 培養細胞 / アマミノクロウサギ / 細胞 / 無限分裂 / 細胞保存 / ゲノム / ゲノム構造 / 無限分裂細胞
Outline of Final Research Achievements	The amami rabbit has an biological characteristics of ancient rabbit. In recent years, genome structure can be clalified on the progress of the next generation sequencing. In this study, we tried to establish the primary cells and immortalized cell derived from amami rabbit. Furthermore, we also tried to clalify the genome struicture of the amami rabbit. We obatined primery cells from 10 roadkilled animals. The primary cells were maintained in the liquid nitrogen tank until use. The PCR detection indicated that we successfully introduced the expression cassettes of mutant CDK4, Cyclin D1, TERT. In the sequential passage, although the primary cell halted the cell proliferation around PD avlue of 40, K4D and K4DT cells showed the continous cell proliferation more than PD value of 90. From these observations, we concluded that we successfully established the immortalized cells from amami rabbit.
Academic Significance and Societal Importance of the Research Achievements	絶滅危惧種であり、我が国の特別天然記念物であるアマミノクロウサギから無限分裂細胞の樹立に成功した。我々の樹立に成功したアマミノクロウサギ由来の細胞は無限分裂し、元の初代培養細胞の性質をある程度保持していると考えられた。

Report

(4 results)