| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2017: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Outline of Final Research Achievements |
The following studies were conducted to measure fluorescence from proteins dissolved in microdroplets with high sensitivity. (1) The electrode for droplet trapping used in the previous studies cannot increase the solid angle of light collection, which makes it difficult to measure fluorescence with high sensitivity. The fluorescence emitted from microdroplets was measured 80 times more sensitively by using the end-cap trap we have developed in this study. (2) We observed the fluorescence enhancement effect caused by the confinement of the excitation light irradiated on the droplet inside the droplet. The sensitivity was improved by about three orders of magnitude compared with the conventional method, together with the improvement of the sensitivity of fluorescence detection by the improvement of the ion trap.
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