|Budget Amount *help
¥111,150,000 (Direct Cost: ¥85,500,000、Indirect Cost: ¥25,650,000)
Fiscal Year 2010: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2009: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2008: ¥28,340,000 (Direct Cost: ¥21,800,000、Indirect Cost: ¥6,540,000)
Fiscal Year 2007: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2006: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
We have developed a highly sensitive and simultaneous method to quantify membrane protein on the basis of separation and identification of protein digestsby liquid chromatography. linked tandem mass spectrometry (LC-MS/MS)with multiplexed multiple/selective reaction monitoring (MRM)mode. The most significant result is that we succeeded to developed an in silico selection criteria to find appropriate peptide fragment to be quantified which will give high intensity by LC-MS/MS analysis. By using this method, we have quantified transporter proteins in the brain capillary endothelial cells, i. e., blood-brain barrier, in mouse. Moreover, we succeeded to clarify quantitative transporter protein expression in the human blood-brain barrier. We identified 11 membrane proteins which may act as transporter in the human blood-brain barrier. These findings will give strong impact for the future blood-brain barrier research, especially for the understanding of effects on the blood-brain barrier function.