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Re-examination of the motile-cyst form relationship based on molecular phylogenetic analysis

Research Project

Project/Area Number 18340166
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Stratigraphy/Paleontology
Research InstitutionNagasaki University

Principal Investigator

松岡 數充  Nagasaki University, 環東シナ海海洋環境資源研究センター, 教授 (00047416)

Co-Investigator(Kenkyū-buntansha) 岩滝 光義 (岩滝 光儀)  長崎大学, 環東シナ海海洋環境資源研究センター, 助教 (50423645)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥13,820,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2006: ¥9,400,000 (Direct Cost: ¥9,400,000)
Keywords分子系統解析 / SSUrDNA / LSUrDNA / 系統分類 / 渦鞭毛藻 / シスト / 単細胞PCR / 層位・古生物 / 分類学 / Polykrikos / Protoperidinium
Research Abstract

従来の研究でも生シストから遺伝子情報を取得する方法はあり, それによって栄養細胞(プランクトン) とシストの対応関係を明らかにする研究はあった. しかし, それらは人工培養が可能な独立栄養性種のみであり, 従属栄養性種について遺伝子情報の抽出は困難とされてきた. 本研究ではこの点を改良すべく単細胞PCR法を活用して渦鞭毛藻の生シストから遺伝子情報を取得する方法の確立に取り組んだ. その結果, 独立栄養性, 従属栄養性を含む8種について生シストから遺伝子情報を取り出すことができた.
この方法を用いてより精度の高い, 確実な遊泳細胞と休眠細胞との対応関係を確立することができた. 上記の種に加え, Cochlodinium cf. geminatumの栄養細胞とシストの対応関係が明らかになった.

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (34 results)

All 2009 2008 2007 2006 Other

All Journal Article (16 results) (of which Peer Reviewed: 13 results) Presentation (18 results)

  • [Journal Article] A new cyst-theca relationship for Protoperidinium parthenopes Zingone & Montresor 1988 (Peridiniales, Dinophyceae)2009

    • Author(s)
      Kawami, H. and Matsuoka, K.
    • Journal Title

      Palynology (Accepted)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protoperidinium tricingulatum sp. nov. (Dinophyceae), a new motile form of a round brown, and spiny dinoflagellate cyst2009

    • Author(s)
      Kawami, H., van Wezel, R., Koeman, R.P.T. and Matsuoka, K.
    • Journal Title

      Phycological Research, (Accepted) (Accepted)

    • NAID

      10027505967

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Re-examination of cyst-motile relationships of Polykrikos kofoidii Chatton and Polykrikos schwartzii Butschli (Gymnodiniales, Dinophyceae).2009

    • Author(s)
      Matsuoka, K., Kawami, H., Nagai, S., Iwataki, M., Takayama, H.
    • Journal Title

      Review of Palaeobotany and Palynology 154

      Pages: 79-90

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Re-examination of cyst-motile relationships of Polykrikos kofoidii Chatton and Polykrikos schwartzii Butschli (Gymnodiniales, Dinophyceae)2009

    • Author(s)
      Matsuoka, K., HKawami, H., Nagai, S., Iwataki, M., Takayama, H.
    • Journal Title

      Review of Palaeobotany and Palynology 掲載確定

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The first appearance of PSP causative dinoflagellate Alexandrium tamarense (Gonyaulacales, Dinophyceae) in Gamak2008

    • Author(s)
      Shin, H.-H., Yoon, Y.-H., Kawami, H., Iwataki, M., and Matsuoka, K.
    • Journal Title

      Bay Korea. Algae 23

      Pages: 251-255

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Re-examination of cyst-motile relationships of Polykrikos kofoidii Chatton and Polykrikos schwartzii Butschli (Gymnodiniales, Dinophyceae)2008

    • Author(s)
      Matsuoka, K., Kawami, H., Nagai, S., Iwataki, M. and Takayama, H.
    • Journal Title

      Review of Palaeobotany and Palynology 154

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The first appearance of PSP causative dinoflagellate Alexandrium tamarense (Gonyaulacales, Dinophyceae) in Gamak, Bay Korea.2008

    • Author(s)
      Shin, H. -H., Yoon, Y. -H., Kawami, H., Iwataki, M., Matsuoka, K.
    • Journal Title

      Algae 23

      Pages: 251-255

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new cyst-theca relationship of Protoperidinium parthenopes Zingone and Montresor (Peridiniales,Dinophyceae).2008

    • Author(s)
      Kawami, H. and Matsuoka, K.
    • Journal Title

      Palynology 32(印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2006渦鞭毛藻シストー栄養細胞対応関係確立への新方法2006

    • Author(s)
      松岡數充・野上規子・川見寿枝・岩滝光儀
    • Journal Title

      化石 No.80

      Pages: 33-40

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new diplopsalid species Oblea acanthocysta sp. nov. (Peridiniales, Dinophyceae)2006

    • Author(s)
      Kawami H., Iwataki, M., and Matsuoka, K.
    • Journal Title

      Plankton & Benthos Research 1

      Pages: 183-190

    • NAID

      10018808547

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Further examination of the cyst-theca relationship of Protoperidinium thulesense (Peridiniales, Dinophyceae) and the phylogenetic significance in round brown cysts.2006

    • Author(s)
      Kazumi Matsuoka, Hisae Kawami, Rika Fujii, Mitsunori Iwataki
    • Journal Title

      Phycologia 45・6

      Pages: 632-641

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A new diplopsalid species Oblea acanthocysta sp. nov. (Peridiniales, Dinophyceae).2006

    • Author(s)
      Hisae Kawami, Mitsunori Iwataki, Kazumi Matsuoka
    • Journal Title

      Plankton and Benthos Research 1・4

      Pages: 183-190

    • NAID

      10018808547

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 渦鞭毛藻シスト-栄養細胞対応関係確立への新方法2006

    • Author(s)
      松岡數充, 野上規子, 川見寿枝, 岩滝光儀
    • Journal Title

      化石 80

      Pages: 33-40

    • NAID

      110004823441

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Protoperidinium tricingulatum sp. nov. (Dinophyceae), a new motile form of a round brown, and spiny dinoflagellate cyst

    • Author(s)
      Kawami, H., van Wezel, R., Koeman, R. P. T., Matsuoka, K
    • Journal Title

      Phycological Research (In press)

    • NAID

      10027505967

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new cyst-theca relationship of Protoperidinium parthenopes Zingone and Montresor (Peridiniales, Dinophyceae)

    • Author(s)
      Kawami, H., Matsuoka, K
    • Journal Title

      Palynology (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Re-examination of cyst-motile form relationships of Polykrikos kofoidii Chatton and Polykrikos schwartzii Butschli (Gymnodiniales, Dinophyceae)

    • Author(s)
      Kazumi Matsuoka, Hisae Kawami, Satoshi Nagai, Mitsunori Iwataki and Haruyoshi Takayama
    • Journal Title

      Review of Palaeobotany and Palynology (Submitted)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 長崎五島産Cochlodinium様無殻渦鞭毛藻1種の形態と系統2008

    • Author(s)
      岩滝光儀・川見寿枝・高野義人・松岡數充
    • Organizer
      日本藻類学会第32回大会
    • Related Report
      2008 Final Research Report
  • [Presentation] DINO8, Morphology and phylogeny of a Cochlodinium-like unarmored dinoflagellate with reference to its cyst-motile cell relationship2008

    • Author(s)
      M. Iwataki, H. Kawami, Y. Takano, S.P.C. Law, S. Lu, Y. Fukuyo and K. Matsuoka
    • Organizer
      DINO8
    • Related Report
      2008 Final Research Report
  • [Presentation] Re-examination of cyst-motile form relationships of Polykrikos kofoidii Chatton and Polykrikos schwartzii Butschli (Gymnodiniales, Dinophyceae)2008

    • Author(s)
      K. Matsuoka, H. Kawami, S. Nagai, M. Iwataki and H. Takayama
    • Organizer
      DINO8
    • Related Report
      2008 Final Research Report
  • [Presentation] Vertical distribution of dinoflagellate cysts in a short core sediment collected from Kota Kinabalu2008

    • Author(s)
      Malaysia. K. Mizushima, M. Iwataki, F. F. Elsa, A. Puyong, B. U. Iris, S. R. Boy, S. Dusit, Y. Fukuyo and K. Matsuoka
    • Organizer
      IOC/WESTPAC 7th International Scientific Symposium
    • Related Report
      2008 Final Research Report
  • [Presentation] Morphology and phylogenetic position of a Cochlodinium-like unarmored dinoflagellate forming resting cyst2008

    • Author(s)
      M. Iwataki, H. Kawami, Y. Takano, S.P.C. Law, S. Lu, Y. Fukuyo and K. Matsuoka
    • Organizer
      IOC/WESTPAC 7th International Scientific Symposium
    • Related Report
      2008 Final Research Report
  • [Presentation] Apical型発芽孔シストをもつ渦鞭毛藻Protoperidinium americanumの系統的位置2008

    • Author(s)
      川見寿枝・岩滝光儀・松岡數充
    • Organizer
      2008年日本プランクトン学会・日本ベントス学会合同大会
    • Related Report
      2008 Final Research Report
  • [Presentation] Morphology and phylogeny of a Cochlodinium-like unarmored dinoflagellate with reference to its cyst-motile cell relationship2008

    • Author(s)
      M. Iwataki, H. Kawami, Y. Takano, S. P. C. Law, S. Lu, Y. Fukuyo, K. Matsuoka
    • Organizer
      DINO8
    • Place of Presentation
      Montreal, Canada
    • Related Report
      2008 Annual Research Report
  • [Presentation] Eutrophication indicators of cysts : Polykrikos kofoidii Chatton and Polykrikos schwartzii Butschli (Gymnodiniales, Dinophyceae).2008

    • Author(s)
      Matsuoka, K.
    • Organizer
      The 6th International Workshop on the Oceanography and Fisheries Science of the East China Sea
    • Place of Presentation
      Nagasaki University, Nagasaki Japan.
    • Related Report
      2008 Annual Research Report
  • [Presentation] A molecular phylogenetic and morphological study of a harmful dinoflagellate Cochlodinium polykrikoides (Dinophyceae)2008

    • Author(s)
      Matsuoka, K., HKawami, H., Nagai, S., Iwataki, M., Takayama, H.
    • Organizer
      The 6th International Workshop on the Oceanography and Fisheries Science of the East China Sea
    • Place of Presentation
      Nagasaki University, Nagasaki Japan.
    • Related Report
      2008 Annual Research Report
  • [Presentation] The International Conference on Harmful Algae (HAB2008), Morphology and phylogenetic position of an unarmored dinoflagellate forming brownish resting cyst2008

    • Author(s)
      M. Iwataki, H. Kawami, Y. Takano, S. P. C. Law, S. Lu, Y. Fukuyo, K. Matsuoka
    • Organizer
      The International Conference on Harmful Algae (HAB2008)
    • Place of Presentation
      Hong Kong
    • Related Report
      2008 Annual Research Report
  • [Presentation] 連鎖群体を形成する無殻渦鞭毛藻Cochlodiniumの形態と分類2007

    • Author(s)
      松岡數充・岩滝光儀・川見寿枝
    • Organizer
      日本藻類学会第31回大会
    • Related Report
      2008 Final Research Report
  • [Presentation] 閉鎖系海域での貧酸素水塊が低次生産系に与える影響2007

    • Author(s)
      松岡數充・中田英昭・山口仁士・万田敦昌・岩滝光儀
    • Organizer
      東京大学海洋研究所共同利用研究集会
    • Related Report
      2008 Final Research Report
  • [Presentation] 従属栄養性渦鞭毛藻Polykrikos kofoidii ChattonとPolykrikos schwartzii Butschliのシストと遊泳細胞の対応関係の再検討2007

    • Author(s)
      松岡數充・川見寿枝・岩滝光儀・長井敏・高山晴義
    • Organizer
      2007年日本ベントス・日本プランクトン学会合同大会
    • Related Report
      2008 Final Research Report
  • [Presentation] Eutrophication indicators of cysts : Polykrikos kofoidii Chatton and Polykrikos schwartzii Butschli (Gymnodiniales, Dinophyceae)2007

    • Author(s)
      K. Matsuoka, H. Kawami, S. Nagai, M. Iwataki and H. Takayama
    • Organizer
      The 6th International Workshop on the Oceanography and Fisheries Science of the East China Sea
    • Related Report
      2008 Final Research Report
  • [Presentation] 従属栄養性渦鞭毛藻Polykrikos kofoidii ChattonとPolykrikos schwartzii Butschliのシストと遊泳細胞の対応関係の再検討.2007

    • Author(s)
      松岡數充
    • Organizer
      日本ベントス・日本プランクトン学会合同大会
    • Place of Presentation
      愛媛大学, 松山
    • Related Report
      2008 Annual Research Report
  • [Presentation] 長崎県大村湾における表層堆積物中の渦鞭毛藻シスト群集の水平分布.2007

    • Author(s)
      仲田希望, 岩滝光儀, 松岡數充
    • Organizer
      日本ベントス・日本プランクトン学会合同大会
    • Place of Presentation
      愛媛大学, 松山
    • Related Report
      2008 Annual Research Report
  • [Presentation] Eutrophication indicators of cysts: Polykrikos kofoidii Chatton and Polykrikos schwartzii Butschli (Gymnodiniales,Dinophyceae)2007

    • Author(s)
      Kazumi Matsuoka, Hisae Kawami, Satoshi Nagai, Mitsunori Iwataki and Haruyoshi Takayama
    • Organizer
      The 6th International Workshop on the Oceanography and Fisherise Science of the East China Sea
    • Place of Presentation
      Nagasaki
    • Related Report
      2007 Annual Research Report
  • [Presentation] Protoperidinium parthenopes(Dinophyceae)のシスト形態と系統的位置2006

    • Author(s)
      川見寿枝・岩滝光儀・松岡數充
    • Organizer
      2006年度日本プランクトン学会・日本ベントス学会合同大会
    • Related Report
      2008 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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