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Development of a Practical SNPs Typing Method by Using Elactro-ActiveArtificial DNAs

Research Project

Project/Area Number 18350082
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionUniversity of Toyama

Principal Investigator

INOUYE Masahiko  University of Toyama, Graduate School of Meddle and Pharmaceutical Sciences, Professor (60211752)

Co-Investigator(Kenkyū-buntansha) FUJIMOTO Kazuhisa  University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Assistant Professor (40334718)
ABE Hajime  University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Assistant Professor (10324055)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,830,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2007: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2006: ¥7,600,000 (Direct Cost: ¥7,600,000)
KeywordsDNA Probe / SNP / Ferrocene / Au Electrode / Electrochemistry
Research Abstract

Most human DNA polymorphisms are single-nucleotide polymorphisms (SNPs), which critically relate to risks of various common diseases and differences in drug response. The clinical importance of detecting SNPs has inspired investigations into numerous assay systems that can discriminate SNP alleles from normal ones. However, the existing systems are not satisfactory, particularly when applied to a hand-held SNPs sensor, which is useful for end users. The ultimate assay protocol must allow end users to only need to directly add the sample that contains the PCR-amplified target DNAs onto a low-cost chip in a portable device.
We have reported an electrochemical detection of complementary DNAs that is based on hole transport with ferrocene-p-conjugated DNA probes. This method allows a reagentless discrimination of label-free DNA from their SNPs with quasi "on/off" digital response. Nevertheless, to meet the criteria of the above-mentioned assay protocol, our method should thoroughly be remad … More e in terms of synthetic cost for the electroactive pseudonucleoside, in situ hybridization of target DNAs on premodified electrode with probes, and applicability to the longer target DNAs than are produced by common PCR. Here we investigated new ferrocene-p-conjugated DNA probes that almost satisfy the requirements for realizing a user-friendly SNP sensor that is highly compatible with PCR on-chip technologies.
We have prepared new DNA probes of various structures and examined their SNP discrimination abilities for target sequences of many transition and transversion SNPs of clinical importance, with the goal of the above-mentioned practical use in mind. As a result, we have developed a new electrochemical SNP typing system composed of synthetically feasible and versatile, cheap electro-active DNA probes. This system is expected to meet the criteria of the assay protocol required by end users majoring in clinical medicines. Further improvements of our methodology are in progress for realizing an ideal SNPs sensor. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (56 results)

All 2008 2007 2006

All Journal Article (19 results) (of which Peer Reviewed: 8 results) Presentation (37 results)

  • [Journal Article] Artificial DNAs Based on Alkynyl C-Nucleosides as a Superior Scaffold for Homo-and Heteroexcimer Emissions2007

    • Author(s)
      Chiba, J.
    • Journal Title

      Chem. -Eur. J. 13

      Pages: 8124-8130

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Photooxygenation of Alkynylperylenes. Fomlation of Dibenzo[jk, mn]phenanthrene-4,5-diones2007

    • Author(s)
      Maeda, H.
    • Journal Title

      J. Org. Chem. 72

      Pages: 8990-8993

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] In Situ, Digital-Like, and Reagentless Discrimination of Label-Free SNPs of 90-mer Length with Easily Synthesized Electrochemical DNA Probes2007

    • Author(s)
      Ikeda, R.
    • Journal Title

      Chem. Bio. Chem 8

      Pages: 2219-2222

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Artificial DNAs Based on Alkynyl C-Nucleosides as a Superior Scaffold for Homo- and Heteroexcimer Emissions2007

    • Author(s)
      Chiba, J., et. al.
    • Journal Title

      Chem.-Eur 13

      Pages: 8124-8130

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Photooxygenation of Alkynylperylenes. Formation of DibenzoUk,mniphenanthrene-4,5-diones2007

    • Author(s)
      Maeda, H., et. al.
    • Journal Title

      J. Org. Chem 72

      Pages: 8990-8993

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] In Situ, Digital-Like, and Reagentless Discrimination of Label-Free SNPs of 90-mer Length with Easily Synthesized Electrochemical DNA Probes2007

    • Author(s)
      Ikeda, R., et. al.
    • Journal Title

      Chem Bio Chem 8

      Pages: 2219-2222

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Artificial DNAs Based no Alkynyl C-Nucleosides as a Superior Scaffold for Homo- and Heteroexcimer Emissions2007

    • Author(s)
      Chiba, J.
    • Journal Title

      Chem.-Eur. J 13

      Pages: 8124-8130

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] photooxygenation of Alkynylperylenes. Formation of dibenzo[jk,mn]phenanthrene-4,5-diones2007

    • Author(s)
      Maeda, H.
    • Journal Title

      J.Org.Chem 72

      Pages: 8990-8993

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In Situ, Digital-Like, and Reagentless Discrimination of label-Free SNPs of 90-mer Length with Syntyesezed Electrochemical DNA Probes2007

    • Author(s)
      Ikeda, R.
    • Journal Title

      ChemBioChem 8

      Pages: 2219-2222

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alkynylpyrenes as Improved Pyrene-Based Biomolecular Probes with the Advantages of High Fluorescence Quantum Yields and Long Absorption/Emission Wavelengths2006

    • Author(s)
      Maeda, H.
    • Journal Title

      Chem. -Eur. J. 12

      Pages: 824-831

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Reversible Photoregulation of Helical Structures in Short Peptides under Indoor Lighting/Dark Conditions2006

    • Author(s)
      Fujimoto, K.
    • Journal Title

      Org. Lett. 8

      Pages: 285-287

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Alkynylpyrenes as Improved Pyrene-Based Biomolecular Probes with the Advantages of High Fluorescence Quantum Yields and Long Absorption/Emission Wavelengths2006

    • Author(s)
      Maeda, H., et. al.
    • Journal Title

      Chem.-Eur 12

      Pages: 824-831

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Reversible Photoregulation of Helical Structures in Short Peptides under Indoor Lighting/Dark Conditions2006

    • Author(s)
      Fujimoto, K., et. al.
    • Journal Title

      Org. Lett 8

      Pages: 285-287

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Alkymylpyrenes as Improved Pyrene-Based Biomolecular Probes with the Advantages of High Fluorescemce Quantum Yields and Long Absorption/Emission Wavelengths2006

    • Author(s)
      Hajime Maeda
    • Journal Title

      Chemistry a European Journal 12

      Pages: 824-831

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Reversible Photoregulation of Helical Structures in Short Peptides under Indoor Lighting/Dark Conditions2006

    • Author(s)
      Kazuhisa Fujimoto
    • Journal Title

      Organic Letters 8

      Pages: 285-287

    • Related Report
      2006 Annual Research Report
  • [Journal Article] SNPを高精度に検出する新手法2006

    • Author(s)
      千葉 順哉
    • Journal Title

      化学 61・3

      Pages: 12-16

    • Related Report
      2006 Annual Research Report
  • [Journal Article] フェロセンを導入した人工DNAによる高精度な電気化学的SNP検出2006

    • Author(s)
      千葉 順哉
    • Journal Title

      日本化学会生体機能関連科学部会NEWS LETTER 20・4

      Pages: 6-9

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 電気化学活性人工DNAによる高精度名電気化学的SNP検出2006

    • Author(s)
      千葉 順哉
    • Journal Title

      BIO INDUSTRY 23・5

      Pages: 46-53

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Helix Formation in Synthetic Polymers by Hydrogen Bonding with Native Saccharides in Protic Media2006

    • Author(s)
      Minoru Waki
    • Journal Title

      Chemistry a European Journal 12

      Pages: 7869-7847

    • Related Report
      2006 Annual Research Report
  • [Presentation] 電気化学活性DNAプローブを用いるデジタル的遺伝子多型検出2008

    • Author(s)
      井上将彦
    • Organizer
      第2回愛媛大学研究開発支援プロジェクト
    • Place of Presentation
      松山
    • Year and Date
      2008-03-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Digital-Like SNPs Detection with Electro-Active DNA Probes2008

    • Author(s)
      Inouye, M., et. al.
    • Organizer
      The Second Research Development Project of Ehime University
    • Place of Presentation
      Matsuyama, Japan
    • Year and Date
      2008-03-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 異なる応答電位を有する2種の電気化学プローブを用いるバイアレル性SNPs遺伝子多型検出2008

    • Author(s)
      北川 哲
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 金電極上に固定したフェロセン修飾オリゴヌクレオチドにおける電荷移動機構の検証2008

    • Author(s)
      池田怜男奈
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 四種類の人工核酸からなるDNA様人工二重らせんの開発2008

    • Author(s)
      土井康広
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Biallelic SNPs Detection by use of Two Types of Electrochemical Probes Possessing Different Potentials2008

    • Author(s)
      Kitagawa, S, et. al.
    • Organizer
      88th Annual Meeting of Chemical Society of Japan
    • Place of Presentation
      Tokyo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Study on Charge Transport Mechanism of Ferrocene-Modified Oligonucleotides on Au Electrodes2008

    • Author(s)
      Ikeda, R., et. al.
    • Organizer
      88th Annual Meeting of Chemical Society of Japan
    • Place of Presentation
      Tokyo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of DNA-Like Duplexes with Four Types of Artificial Bases2008

    • Author(s)
      Doi, Y., et. al.
    • Organizer
      88th Annual Meeting of Chemical Society of Japan
    • Place of Presentation
      Tokyo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アルキニルC-ヌクレオチドを基本骨格とする人工DNAの開発2007

    • Author(s)
      千葉順哉
    • Organizer
      日本薬学会北陸支部第117回例会
    • Place of Presentation
      金沢
    • Year and Date
      2007-11-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Development of Artificial DNAs Based on Alkynyl C-Nucleotides2007

    • Author(s)
      Chiba, J., et. al.
    • Organizer
      117th Hokuriku Branch Meeting for Ph armaceutical Society of Japan
    • Place of Presentation
      Kanazawa, Japan
    • Year and Date
      2007-11-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Electrochemical Detection of Single-Base Mismatches in DNA with Ferrocene-π-Conjugated DNA Probes2007

    • Author(s)
      Ikeda, R.
    • Organizer
      International Conference on Molecular Machines and Sensors
    • Place of Presentation
      Shanghai, China
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Mismatch Detection Based on Hole Transport with Easily Available, Ferrocene-π-Conjugated DNA Probes2007

    • Author(s)
      Chiba, J.
    • Organizer
      International Conference on Molecular Machines and Sensors
    • Place of Presentation
      Shanghai, China
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Intramolecular Homo-and Heteroexcimer Emissions of Polycyclic Aromatic Hydrocarbons in Aqueous Media2007

    • Author(s)
      Chiba, J.
    • Organizer
      12th International Symposium on Novel Aromatic Compounds
    • Place of Presentation
      Awaji, Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Photooxygenation of Alkynylperylenes. Formation of Dibenzo [jk, mn]phenanthrene-4, 5-diones2007

    • Author(s)
      Maeda, H.
    • Organizer
      2007 Korea-Japan Symposium on Frontier Photoscience
    • Place of Presentation
      Gyeongju, Korea
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA Probes Relied on Charge Transport through the π-Stack of Duplexes2007

    • Author(s)
      井上将彦
    • Organizer
      分子系の構造と電子状態-「生物物質科学」を目指して
    • Place of Presentation
      和光
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] デジタル応答を有する電気化学的SNPs検出プローブの開発2007

    • Author(s)
      井上将彦
    • Organizer
      第6回国際バイオフォーラム
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 金電極上でのDNA媒介電荷輸送メカニズムの解明2007

    • Author(s)
      小林沙希絵
    • Organizer
      第19回生体機能関連化学若手の会サマースクール
    • Place of Presentation
      八王子
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] デジタル応答を有するSNPs検出プローブの開発2007

    • Author(s)
      井上将彦
    • Organizer
      イノベーションジャパン2007
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 四種類の人工核酸を導入したアルキニルC-オリゴヌクレオチドの開発2007

    • Author(s)
      土井康広
    • Organizer
      第22回生体機能関連化学シンポジウム
    • Place of Presentation
      仙台
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] デジタル的な2電位応答を利用したSNPsタイピング法の開発2007

    • Author(s)
      北川 哲
    • Organizer
      第22回生体機能関連化学シンポジウム
    • Place of Presentation
      仙台
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] イソキノリンを導入したフェロセン修飾DNAプローブによる電気化学的SNPs検出および遺伝子型判定2007

    • Author(s)
      赤石あゆみ
    • Organizer
      第22回生体機能関連化学シンポジウム
    • Place of Presentation
      仙台
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] バイアレルのSNPs検出を指向したフェロセン修飾DNAプローブの開発2007

    • Author(s)
      北川 哲
    • Organizer
      平成19年度有機合成化学北陸セミナー
    • Place of Presentation
      金沢
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Electrochemical Detection of Single-Base Mismatches in DNA with Ferrocene-n-Conjugated DNA Probes2007

    • Author(s)
      Ikeda, R., et. al.
    • Organizer
      International Conference on Molecular Machines and Sensors
    • Place of Presentation
      Shanghai, China
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mismatch Detection Based on Hole Transport with Easily Available, Ferrocene-n-Conjugated DNA Probes2007

    • Author(s)
      Chiba, J., et. al.
    • Organizer
      International Conference on Molecular Machines and Sensors
    • Place of Presentation
      Shanghai, China
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Intramolecular Homo- and Heteroexcimer Emissions of Polycyclic Aromatic Hydrocarbons in Aqueous Media2007

    • Author(s)
      Chiba, J., et. al.
    • Organizer
      12th Internatinal Symposium on Novel Aromatic Compounds
    • Place of Presentation
      Awaji, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Photooxygenation of Alkynylperylenes. Formation of Dibenzopc,mn]phenanthrene-4,5-diones2007

    • Author(s)
      Maeda, H., et. al.
    • Organizer
      2007 Korea-Japan Symposium on Frontier Photoscience
    • Place of Presentation
      Gyeongju, Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA Probes Relied on Charge Transport through the n-Stack of Duplexes2007

    • Author(s)
      Inouye, M., et. al.
    • Organizer
      Structure and Electronic Characteristics of Molecular Systems
    • Place of Presentation
      Wako, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of Electrochemical SNPs Probes with a Digital Action2007

    • Author(s)
      Inouye, M., et. al.
    • Organizer
      6th International Bio-Forum
    • Place of Presentation
      Tokyo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Study on DNA-Mediated Charge Transport on Au Electrode2007

    • Author(s)
      Kobayashi, S., et. al.
    • Organizer
      19th Summer School on Bioorganic Chemistry
    • Place of Presentation
      Hachjoji, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of SNPs Detection Probe Possessing a Digital Action2007

    • Author(s)
      Inouye, M., et. al.
    • Organizer
      Innovation Japan
    • Place of Presentation
      Tokyo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of C-Nucleotides Bearing Four Types of Artificial Bases2007

    • Author(s)
      Doi, Y., et. al.
    • Organizer
      22th Symposium on Bioorganic Chemistry
    • Place of Presentation
      Sendai. Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of SNPs-Typing Method with Digital-Like Two Potential Responses2007

    • Author(s)
      Kitagawa, S., et. al.
    • Organizer
      22th Symposium on Bioorganic Chemistry
    • Place of Presentation
      Sendai, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Electrochemical SNPs and Genotype Detection with Ferrocene-Modified DNA Probes Possessing an Isoquinoline Base2007

    • Author(s)
      Akaishi, A. et. al.
    • Organizer
      22th Symposium on Bioorganic Chemistry
    • Place of Presentation
      Sendai, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of Ferrocene-Modified DNA Probes Directed toward Biallelic SNPs Detection2007

    • Author(s)
      Kitagawa, S., et. al.
    • Organizer
      19th Hokuriku Seminar on Organic Chemistry
    • Place of Presentation
      Kanazawa, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mismatch Detection Based on Hole Transport with Easily Available, Ferrocene-π-Conjugated DNA Probes2007

    • Author(s)
      Chiba, J.
    • Organizer
      International Conference on Molecular Machines and Sensors
    • Place of Presentation
      Shanghai,China
    • Related Report
      2007 Annual Research Report
  • [Presentation] Intramolecular Homo- and Heteroexcimer Emissions of Polycyclic Aromatic Hydrocarbons in Aqueous Media2007

    • Author(s)
      Chiba, J.
    • Organizer
      12th International Symposium on Novel Aromatic Compounds
    • Place of Presentation
      Awaji, Japan
    • Related Report
      2007 Annual Research Report
  • [Presentation] Phtooxygenation of Alkynylperylenes. Formation of Dibenzo[jk,mn]phenantherne-4,5-diones2007

    • Author(s)
      Maeda, H.
    • Organizer
      2007 Korea-Japan Symposium on Frontier Photoscience
    • Place of Presentation
      Gyeongju, Korea
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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