Signal transduction pathways regulating cortical microtubule functions
| Project/Area Number |
18370020
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
HASHIMOTO Takashi Nara Institute of Science and Technology, Graduate School of Biological Sciences, Professor (80180826)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥17,170,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2007: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2006: ¥8,200,000 (Direct Cost: ¥8,200,000)
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| Keywords | Arabidopsis / microtubule / signal transduction / phosphorylation |
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| Research Abstract |
Many extragenic suppressors were obtained after EMS-mutagenesis of the semi-dominant phs1-1 allele. A group of suppressors did not follow a Mendelian segregation and could not be mapped in this study. Other suppressors were shown to have mis-sense mutations in Arabidopsis tubulin genes.
Full length PHS1 cDNAs of wild type and the phs1-1-type mutantation were used to screen Arabidopsis cDNA libraries by a yeast two-hybrid method, bud did not result in positive clones.
When a phosphatase-dead mutant of PHS1 was expressed in plant cells, it strongly induced depolymerization of cortical microtubules. This dominant-negative effect was specific to PHS1 since similar phosphatase-dead mutants of other four Arabidopsis MAP kinase phosphatases did not affect cortical microtubule organization. PHS1 is positively retained in the cytoplasm. A C-terminal region excluding the phosphatase catalytic domain was necessary and sufficient for the cytoplasmic retention. Possibly, PHS1 may dephosporylate microtubule-related factors in the cytoplasm.
While screening downstream targets of PHS1, we found that Ser-172 of β-tubulin is phosphorylated, cyclin-dependent kinases catalyze this phosphorylation, and the phosphorylated b-tubulin significantly accumulates in an Arabidopsis ton2 mutant of a regulatory subunit in the PP2A phosphatase.
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Report
(3 results)
Research Products
(20 results)
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[Presentation] 微小管構築の遺伝学的解析2008
Author(s)
橋本 隆
Organizer
日本植物生理学会年会
Place of Presentation
札幌
Year and Date
2008-03-20
Description
「研究成果報告書概要(和文)」より
Related Report
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