Growth and toxin production of causative dinoflgellates for paralytic shellfish poisoning in the environment of bivalve culture farm

• Project/Area Number: 18380125
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Kitasato University
• Principal Investigator: OGATA Takehiko Kitasato University, 海洋生命科学部, 教授 (00104521)
• Co-Investigator(Kenkyū-buntansha): SATO Shigeru 北里大学, 海洋生命科学部, 准教授 (20170748) ; KOBIYMA Atsushi 北里大学, 海洋生命科学部, 講師 (60337988)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,130,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥1,230,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2006: ¥10,800,000 (Direct Cost: ¥10,800,000)
• Keywords: 麻ひ性貝毒 / 渦鞭毛藻 / 養殖 / 二枚貝 / 生長 / 毒生産 / Alexandrium / 栄養塩 / 栄養摂取 / 自家蛍光顆粒 / アンモニア / 麻痺性貝毒 / ホタテガイ / 栄養環境 / 増殖

Research Abstract

二枚貝養殖環境では排泄物を通じて栄養成分が供給され、これら栄養環境に応答して麻痺性貝毒原因渦鞭毛藻の生長、毒生産が促進されること、海藻など栄養塩競合生物を共存させることにより促進効果を抑制できることが明らかとなった。また、今後貝類養殖業の振興が予想される東南アジア諸国において出現するまひ性貝毒原因藻の発生状況と特性を調べ、将来に向けた基礎データを収集した。

Research Products

• [Journal Article] Toxic Alexandrium minutum(Dinophyceae) from Vietnam with new gonyautoxin analogue (2007)
  • Author(s): Lim PT, Sato S, Thuoc CV, Tu PT, Nguyen TMH, Takata Y, Yoshida M, Kobiyama A, Koike K, Ogata T
  • Journal Title: Harmful Algae 6 Pages: 321-331
  • Peer Reviewed
• [Journal Article] Morphological variation of two Alexandriumspecies responsible for paralytic shellfish poisoning in Southeast Asia (2007)
  • Author(s): Lim PT, Leaw CP, Ogata T
  • Journal Title: Botanica Marina 50 Pages: 14-21
  • Peer Reviewed
• [Journal Article] Isolation of a differentially expressed gene in separate mating types of the dinoflagellate Alexandrium tamarense (2007)
  • Author(s): Kobiyama A, Ikeda Y, Koike K, Ogata T
  • Journal Title: European Journal of Phycology 42 Pages: 183-190
  • Peer Reviewed
• [Journal Article] Toxic Alexandrium minutum (Dinophyceae)from Vietnam with new gony-autoxin analogue (2007)
  • Author(s): Lim PT, 他9名
  • Journal Title: Harmful Algae 6 Pages: 321-331
  • Peer Reviewed
• [Journal Article] Morphological variation of two Alexandrium responsible for paralytic shellfish poisoning in South Asia (2007)
  • Author(s): Lim PT, 他2名
  • Journal Title: Botanica Marina 50 Pages: 14-21
  • Peer Reviewed
• [Journal Article] Isolation of a differentially expressed gene in separate mating types of the dinoflagellate Alexandrium tamarense. (2007)
  • Author(s): Kobiyama A, 他3名
  • Journal Title: European Journal of Phycology 42 Pages: 183-190
  • Peer Reviewed
• [Journal Article] Effects of light and temperature on growth, nitrate uptake and toxin production of two tropical dinoflagellates, Alexandrium tamiyavanichiiand Alexandrium minutum(Dinophyceae) (2006)
  • Author(s): Lim PT, Leaw CP, Usup G, Kobiyama A, Koike K, Ogata T
  • Journal Title: Journal of Phycology 42 Pages: 786-799
  • Peer Reviewed
• [Journal Article] Seasonal variation of paralytic and amnesic shellfish toxicities in bivalves and microalgae in Haiphong area, Vietnam (2006)
  • Author(s): Nguyen TMH, Thuoc CV, Ogata T, SatoS, Takata Y, Kodama M, Fukuyo Y
  • Journal Title: Coastal Marine Science 30 Pages: 111-115
  • Peer Reviewed
• [Journal Article] 岩手県沿岸に出現するAlexandrium属とその毒生産能 (2006)
  • Author(s): 加賀新之助, 関口勝司, 吉田誠, 緒方武比古
  • Journal Title: 日本水産学会誌 72 Pages: 1068-1076
  • NAID: 110006150286
  • Peer Reviewed
• [Journal Article] Effects of light and temperature on growth, nitrate uptake and toxin production of two tropical dinoflagellates, Alexandrium tamiyavanichii and Alexandrium minutum (Dinophyceae). (2006)
  • Author(s): Lim, P.T., Leaw, C.P., Usup, G., Kobiyama, A., Koike, K., Ogata, T.
  • Journal Title: Journal of Phycology 42巻 Pages: 786-799
• [Journal Article] Seasonal variation of paralytic and amnesic shellfish toxicities in bivalves and microalgae in Haiphong area, Vietnam (2006)
  • Author(s): Nguyen, T.M.H., Thuoc, C.V, Ogata, T., Sato, S., Takata, Y., Kodama M., Fukuyo, Y.
  • Journal Title: Coastal Marine Science 30巻 Pages: 111-115
• [Journal Article] 岩手県沿岸に出現するAlexandrium属とその毒生産能 (2006)
  • Author(s): 加賀新之助, 関口勝司, 吉田 誠, 緒方武比古
  • Journal Title: 日本水産学会誌 72巻 Pages: 1068-1076
  • NAID: 110006150286
• [Presentation] 岩手県沿岸域における主要カイアシ類2種の有毒渦鞭毛藻Alexandrium tamarense に対する摂餌活性 (2009)
  • Author(s): 加藤千晶、山田雄一郎、小檜山篤志、緒方武比古
  • Organizer: 2009年度日本海洋学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-06
• [Presentation] 岩手県沿岸域における主要カイアシ類2種の有毒渦鞭毛藻 Alexandrium tamarenseに対する摂餌活性 (2009)
  • Author(s): 加藤千晶, 山田雄一郎, 小檜山篤志, 緒方武比古
  • Organizer: 2009年度日本海洋学会春季大会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2009-04-06
• [Presentation] 渦鞭毛藻Alexandrium tamarenseのアンモニウム輸送体(AMT)に関する研究 (2009)
  • Author(s): 沖山晋吉、小檜山篤志、緒方武比古
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-30
• [Presentation] 有毒渦鞭毛藻Alexandriumtamarenseの細胞内自家蛍光顆粒に関する研究 (2009)
  • Author(s): 篠原なつみ、伊早坂ゆかり、山田雄一郎、小檜山篤志、緒方武比古、加賀新之助
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-30
• [Presentation] 渦鞭毛藻Alexandrium tamarenseのアンモニウム輸送体(AMT)に関する研究 (2009)
  • Author(s): 沖山晋吉, 小檜山篤志, 緒方武比古
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
  • Year and Date: 2009-03-30
• [Presentation] 有毒渦鞭毛藻Alexandrium tamarenseの細胞内自家蛍光顆粒に関する研究 (2009)
  • Author(s): 篠原なつみ, 伊早坂ゆかり, 他4名
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
  • Year and Date: 2009-03-30
• [Presentation] Molecular cloning and expression analysis of transketolase in the dinoflagellate Alexandrium tamarense (2008)
  • Author(s): Okiyama S, Kobiyama A, Ogata T
  • Organizer: 5^<th> World Fisheries Congress
  • Place of Presentation: Yokohama
  • Year and Date: 2008-10-23
• [Presentation] Effect of calcium ion on cyst formation of Alexandrium tamarense (2008)
  • Author(s): Kobiyama A, Ogata T
  • Organizer: 5^h World Fisheries Congress
  • Place of Presentation: Yokohama
  • Year and Date: 2008-10-23
• [Presentation] Biochemical degradation of C11-O-sulfate paralytic shellfish poisoning toxins (2008)
  • Author(s): Sato S, Sakata E, Aburatani H,Hayashi T, Kodama M
  • Organizer: 5^<th> World Fisheries Congress
  • Place of Presentation: Yokohama
  • Year and Date: 2008-10-21
• [Presentation] 渦鞭毛藻Alexandrium tamarense遺伝子の発現機構の解析 (2008)
  • Author(s): 小檜山篤志、角南玲子、山田雄一郎、緒方武比古
  • Organizer: 平成20年度日本水産学会春季大会
  • Place of Presentation: 清水
  • Year and Date: 2008-03-30
• [Presentation] 渦鞭毛藻Alexandrium tamarense遺伝子の発現解析 (2008)
  • Author(s): 小檜山 篤志, 他3名
  • Organizer: 日本水産学会
  • Place of Presentation: 東海大学
  • Year and Date: 2008-03-30
• [Presentation] Biochemical degradation of CII-0-sulfate paralytic shellfish poisoning toxins (2008)
  • Author(s): S Sato, 他4名
  • Organizer: 5^<th> World Fisheries Congress
  • Place of Presentation: 横浜パシフイコ(横浜市)
• [Presentation] Molecular cloning and expression analysis of trans-Ketolase in the dinoflagellate Alexandrium tamarense (2008)
  • Author(s): S Okiyama, A Kobiyama, T Ogata
  • Organizer: 5^<th> World Fisheries Congress
  • Place of Presentation: 横浜パシフィコ(横浜市)
• [Presentation] Effect of calcium ion on cyst formation of Alexandrium tamarense (2008)
  • Author(s): A Kobiyama, T Ogata
  • Organizer: 5^<th> World Fisheries Congress
  • Place of Presentation: 横浜パシフィコ(横浜市)
• [Presentation] Growth responses of five non paralytic shellfish toxins (PSTs) producing Alexandrium species (Dinophyceae) to temperature and salinity (2007)
  • Author(s): Lim PT, Leaw CP, Kaga S, Sekiguchi S,Sato S, Kobiyama A, Ogata T
  • Organizer: LIPI-JSPS Joint Seminar on Coastal Marine Science
  • Place of Presentation: Yogyakarta, Indonesia
  • Year and Date: 2007-08-04
• [Presentation] 渦鞭毛藻Alexandrium tamarenseにおけるuncoupling progein様遺伝子のクローニングと発現解析 (2007)
  • Author(s): 小檜山篤志、田中将平、緒方武比古
  • Organizer: 第10回マリンバイオテクノロジー学会大会
  • Place of Presentation: 山形
  • Year and Date: 2007-05-27
• [Presentation] 渦鞭毛藻Alexandrium tamarenseにおけるuncoupling protein様遺伝子のクローニングと発現解析 (2007)
  • Author(s): 小檜山 篤志, 他2名
  • Organizer: マリンバイオテクノロジー学会
  • Place of Presentation: 山形大学
  • Year and Date: 2007-05-27
• [Presentation] Effect of nitrogen and phosphorus ratios on growth and toxin production of dinoflagellate Alexandrium minutum(Dinophyceae) (2007)
  • Author(s): Lim PT, Leaw CP, 小檜山篤志、小池一彦、緒方武比古
  • Organizer: 平成19年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2007-03-30
• [Presentation] Alexandrium tamarense自然集団における生活史と毒生産の関係 (2007)
  • Author(s): 加賀新之助、小檜山篤志、小池一彦、緒方武比古
  • Organizer: 平成19年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2007-03-30
• [Presentation] Isolation preferentially expressed gene between different mating type cells in the dinoflagellated Alexandriumtamarense (2006)
  • Author(s): Kobiyama A, Koike K, Ogata T,
  • Organizer: 12th International Conference on Harmful Algae
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2006-09-07
• [Presentation] New gonyautoxin analogue isolated from the toxic dinoflagellate Alexandrium minutum(Dinophyceae) (2006)
  • Author(s): Lim PT, Sato S, Thuoc CV, Nguyen TMH, Takata Y, Yoshida M, Kobiyama A, Koike K, Ogata T
  • Organizer: 12^<th> International Conference on Harmful Algae
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2006-09-07
• [Presentation] Growth and toxin production of the dinoflagellate, Alexandrium minutum(Dinophyceae) isolated from Tumpat esturary, northeastern part of peninsula Malaysia (2006)
  • Author(s): Ogata T, Leaw CP, Usup G, Kobiyama A, Koike K, Lim PT
  • Organizer: 12^<th> International Conference on Harmful Algae
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2006-09-07
• [Presentation] 渦鞭毛藻Alexandrium tamarenseの温度応答 (2006)
  • Author(s): 田中将平、小檜山篤志、金子豊、小池一彦、緒方武比古
  • Organizer: 平成18年度日本水産学会大会
  • Place of Presentation: 高知
  • Year and Date: 2006-03-31
• [Presentation] 麻痺性貝毒原因渦鞭毛藻Alexandriumの休眠過程と毒成分変化 (2006)
  • Author(s): 緒方武比古、伊東詩織、坪井優卓、小檜山篤志、小池一彦
  • Organizer: 平成18年度日本水産学会大会
  • Place of Presentation: 高知
  • Year and Date: 2006-03-31
• [Book] New York、Seafood and Freshwater Toxins : Pharmacology, Physiology and Detection、(担当章題名 : Chaper 7 - Metabolism of paralytic shellfish toxinsincorporated into Bivalves.) (2008)
  • Author(s): Kodama M, Sato S
  • Publisher: CRC Press
• [Book] Seafood and Freshwater Toxins:Pharmacology, Physiology and Detection (担当章題名:Chaper 7-Metabolism of paralytic shellfish toxins incorporated into Bivalves.) (2008)
  • Author(s): Kodama M, Sato S
  • Publisher: CRC Press, New York