Study on the origin of paralytic shellfish poisoning toxins ----a new approach using specific antibody against saxitoxin

• Project/Area Number: 18380126
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Kitasato University
• Principal Investigator: KODAMA Masaaki Kitasato University, 海洋生命科学部, 教授 (40050588)
• Co-Investigator(Kenkyū-buntansha): 佐藤 繁 北里大学, 海洋生命科学部, 准教授 (20170748)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,950,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥2,550,000); Fiscal Year 2008: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2007: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2006: ¥6,900,000 (Direct Cost: ¥6,900,000)
• Keywords: 細菌、saxitoxin(STX) / gonyautoxin(GTX) / RNA / 抗STX抗体 / affinity column / RNaseP1 / 麻ひ性貝毒 / 麻ひ性貝毒生産菌 / 抗saxitoxin抗体 / 抗体結合affinity column / gonyautoxin 2,3 / saxitoxin / nuclease P1 / 麻ひ性貝毒結合rRNA / PSP生産菌PTB-1 / protease消化 / 抗STXウサギ抗体 / ELISA / western blotting / Alexandrium tamarense

Research Abstract

本研究の目的は、これまで植物プランクトンが生産すると考えられてきた麻ひ性貝毒(PSP)がプランクトンに共生あるいは寄生する細菌生体成分の構成成分であるという筆者らの仮説の証明にある。本研究では筆者らが開発した毒に対する特異抗体を用い、毒を結合している生体成分を分離し、同成分が細菌RNAの一部であることを明らかにした。このことはPSPは細菌の生命維持に欠くことのできない物質の構成要素であることを示唆する。

Research Products

• [Journal Article] Domoic acid in small-sized plankton in Nha Phu Bay (2009)
  • Author(s): Dao, V.H., Takata, Y., Omura, T., Nguyen, T.D., Nguyen, T.H., Sato, S., Fukuyo, Y., Kodama, M.
  • Journal Title: Khanh Hoa Province, Vietnam. La mer (in print)
• [Journal Article] Seasonal variation of domoic acid in Spondylus versicolor in association with that in plankton samples in Nha Phu Bay (2009)
  • Author(s): Dao, V.H., Takata, Y., Omura, T., Sato, S., Fukuyo, Y., Kodama, M.
  • Journal Title: Khanh Hoa, Vietnam. Fisheries Science 75 Pages: 507-512
• [Journal Article] Occurrence of domoic acid in tropical bivalves (2009)
  • Author(s): Takata, Y., Sato, S., Dao, V.H., Montojo,U.M. ,Lirdwitayaprasit,T., Kamolsiripichaiporn, S., Kotaki, Y., Fukuyo, Y., Kodama, M.
  • Journal Title: Fisheries Science 75 Pages: 472-480
  • NAID: 10024970450
• [Presentation] PSP産生菌における抗PSP抗体と反応するRNA (2009)
  • Author(s): 児玉正昭, 他
  • Organizer: 平成21年度日本水産学科春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2009-03-30
• [Presentation] 海洋細菌に認められた抗STX抗体と反応するRNA (2009)
  • Author(s): 児玉正昭, 他
  • Organizer: 平成21年度日本水産学科春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2009-03-27
• [Presentation] Proteins bound with saxitoxin-analogue, a key substance for the study on metabolism of PSP toxins (Invited paper) (2008)
  • Author(s): 児玉正昭, 他
  • Organizer: World Congress on Harmful Algal Bloom
  • Place of Presentation: 香港
  • Year and Date: 2008-11-21
• [Presentation] Bacterial proteins which react with specific antibody against saxitoxin (2008)
  • Author(s): 児玉正昭, 他
  • Organizer: 5^<th> World Fisheries Congress
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2008-10-21
• [Presentation] Proteins bound with saxitoxin-analogue, a key substance for the study on metabolism of PSP toxins (2008)
  • Author(s): Kodama, M., Takata, Y., Osako, T., Sato, S.
  • Organizer: The abstract of 13^<th> international conference on harmful algae
  • Place of Presentation: Hong Kong
• [Presentation] Bacterial proteins which react with antibody against saxitoin (2008)
  • Author(s): Osako, T., Takata, Y., Sato, S., Kodama, M.
  • Organizer: 5 th World Fisheries Congress
  • Place of Presentation: Yokohama Japan
• [Presentation] Bacterial proteins which react with antibody against domoic acid. (2008)
  • Author(s): Takata, Y., Kodama, M.
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Yokohama Japan
• [Presentation] An enzyme immunosorbent assay as alternative method of mouse test to detect shellfish poisoning toxins (2008)
  • Author(s): Sato, S., Kondo, S., Takata, Y., Kodama, M., Fukuyo, Y.
  • Organizer: The abstract of 7^<th>IOC/WESTPAC international scientific symposium
  • Place of Presentation: Kota Kinabalu
• [Presentation] 麻痺性貝毒に対する抗体と反応する細菌成分 (2006)
  • Author(s): 児玉正昭・高田義宜・Ulysses M. Montojo・新保昌康・佐藤繁
  • Organizer: 日本水産学会春季大会高知大学
• [Book] Metabolism of paralytic shellfish toxins incorporated into bivalve In Seafood and Freshwater Toxins:Pharmacology, Physiology, and Detection (2008)
  • Author(s): M.Kodama and S.Sato
  • Publisher: Taylor and Francis Group, LLC(ed.L.M.Botana)(In press)
• [Book] Relationships between Bacteria and Harmful Algae (Edna Graneli, J.T Turner Eds.) Ecology of Harmful Algae Germany Springer-Verlag GmbH Relationships between Bacteria and Harmful Algae (Edna Graneli, J.T Turner Eds.) Ecology of Harmful Algae Germany Springer-Verlag GmbH (2006)
  • Author(s): Kodama, M, Doucette, G.J, Green, D.H
• [Book] Ecology of Harmful Algae, Ecological Studies, Vol. 189, 243-255 (2006)
  • Author(s): M.Kodama, G.J.Doucette, D.H.Green
  • Total Pages: 413
  • Publisher: Springer-Verlag Berlin Heidelberg