Study on molecules which exhibit either inhibitory or stimulating activities to growth of pathogen, especially Vibribparahaemolyticus
| Project/Area Number |
18390135
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
HONDA Takeshi Osaka University, Research Institute for Microbial Diseases, Professor (60029808)
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| Co-Investigator(Kenkyū-buntansha) |
KODAMA Toshio Osaka University, Research Institute for Microbial Diseases, Assistant Professor (20346133)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,830,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2007: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2006: ¥7,600,000 (Direct Cost: ¥7,600,000)
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| Keywords | Serum / growth factor / inhibitor / Vibiro narahaemolvticus / transferin / hemonexin |
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| Research Abstract |
We found the activities which inhibit or stimulate the growth of bacteria in bovine serum and human serum as well. Inhibitory molecule for the bacterial growth (especially Vibrio parahaemolyticus) was purified by successive column chromatographies of Sephadex G75 and DEAE-Sephadex G25 and Mono Q (FPLC), and we obtained the molecules of either transferin or hemopexin or both. On the other hand, we are close to obtain highly purified molecule which exhibits stimulating activity to bacterial growth. The molecular weight seems to be less than 3000, and it is heat-stable (at 100℃). It contains Mg+ and Mo+. In conclusion, we found that there are still unidentified factors in sera which exhibit either inhibitory on stimulating activities on growth of pathogen.
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Report
(3 results)
Research Products
(52 results)
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[Journal Article] Translocation of VP1686 Upregulates RhoB and Accelerates Phagocytic Activity of Macrophage Through Actin Remondeling.2008
Author(s)
B., Rabindra, N., Kwon-Sam, Park, X., Chen, T., Iida, T., Honda, O., Takeuchi, Akira
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Journal Title
J.Microbiol.Biotechnol 18.1
Pages: 171-175
Description
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[Journal Article] Comparative Genomic Analysis Using Microarray Demonstrates a Strong Correlation between the Presence of the 80-Kilobase Pathogenicity Island and Pathogenicity in Kanagawa Phenomenon-Positive Vibrio parahaemolyticus Strains.2008
Author(s)
Kaori, Izutsu, Ken, Kurokawa, Kosuke, Tashiro, Satoru, Kuhara, Tetsuya, Hayashi, Takeshi, Honda, Tetsuya, Iida
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Journal Title
Infection and Immunity 76(3)
Pages: 1016-1023
Description
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[Journal Article] Tetrameric structure of thermostable direct hemolysin from Vibrio parahaemolyticus revealed by ultracentrifugation, small-angle x-ray scattering and electron microscopy.2007
Author(s)
Hamada., D., T., Higurashi, K., Mayanagi, T., Miyata, T., Fukui, T., Iida, T., Honda, I., Yanagihara
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Journal Title
J. Mol. Biol 365
Pages: 365-365
Description
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[Journal Article] Polyclonality of Staphylococcus epidermidis residing on the healthy ocular surface2007
Author(s)
Ueta., M., T., Iida, M., Sakamoto, C., Sotozono, J., Takahashi, K., Kojima, K., Okada, X., Chen, S., Kinoshita, T., Honda
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Journal Title
J. Med.Microbiol 56
Pages: 77-82
Description
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[Journal Article] Tyrosine phosphorylation controls cortactin binding to two enterohaemorrhagic Escherichia coli effectors : Tir and EspFu/TccP.2007
Author(s)
Vlademir, V., Cantarelli, T., Kodama, N., Nijstad, S., K., Abolghait, S., Nada, M., Okada, Tetsuya, Iida, T., Honda
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Journal Title
Cellular Microbiology 9(7)
Pages: 1782-1795
Description
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[Journal Article] Cortactin is essential for F-actin assembly in enteropathogenic Escherichia co/i(EHEC)-and enterohaemorrhagic E. coli (EHEC)-induced pedestals and the a-helical region is involved in the localization of cortactin to bacterial attachment sites.2006
Author(s)
Vlademir., C., T., Kodama, N., Nijjstad, S.K., Abolghait, T., lida, T., Honda
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Journal Title
Cellular Microbiology 8(5)
Pages: 769-780
Description
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[Journal Article] Two type IV pili of Vibrio parahaemolyticus play different roles in biofilm formation2006
Author(s)
Shime., A., H.T., Iida, M., Arita, K.S., Park, T., Kodama, T., Honda
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Journal Title
FEMS Microbiol. Lett 264
Pages: 89-97
Description
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[Journal Article] Pore formation of thennostable Vibrio parahaemolyticus in lipid bilayers.2006
Author(s)
Takahashi, A, Yamamoto, C, Kodama, T, Yamashita, K, Harada, N, Nakano, M, Honda, T, Nakaya, Y
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Journal Title
Int J Toxicol 25(5)
Pages: 409-418
Description
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[Presentation] Identification of two translocon proteins of Vibrio parahaemolyticus type III secretion system 2.2007
Author(s)
Toshio, Kodama, Hirotaka, Hiyoshi, Shigeaki, Matsuda, Yukihiro, Akeda, Tetsuya, Iida, Takeshi, Honda
Organizer
Cholera & Other Bacterial Enteric Infections. 42nd Annual Joint Panel Meeting
Place of Presentation
Austin, Texas
Description
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[Presentation] Identification and characterization of type III secretion-associated chaperone of Vibrio parahaemolyticus.2007
Author(s)
Yukihiro, Akeda, Kanna, Okayama, Toshio, Kodama, Tetsuya, Iida, Takeshi, Honda
Organizer
Cholera & Other Bacterial Enteric Infections. 42nd Annual Joint Panel Meeting
Place of Presentation
Austin, Texas
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Two type IV pili of Vibrio parahaemolyticus play different roles in biofilm formation.2006
Author(s)
Akiko, Shime-Hattori, Tetsuya, Iida, Michiko, Arita, Kwon-Sam, Park, Toshio, Kodama, Takeshi, Honda
Organizer
41st Joint Conference on Cholera and Other Bacterial Enteric Infections Pannel
Place of Presentation
Gifu
Description
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[Presentation] Identification and characterization of VopT, a novel ADP-ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type HI secretion system 2.2006
Author(s)
Toshio, Kodama, Mitsuhiro, Rokuda, Kwon-Sam, Park, Vlademir, V., Cantarelli, Tetsuya, Iida, Takeshi, Honda
Organizer
41st Joint Conference on Cholera and Other Bacterial Enteric Infections Pannel
Place of Presentation
Gifu
Description
「研究成果報告書概要(欧文)」より
Related Report
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