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Elucidation of the aroma formation of Darjeeling tea "Second flash" and development of new manufacturing process of black tea

Research Project

Project/Area Number 18405027
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionKyoto University

Principal Investigator

MIZUTANI Masaharu (2007)  Kyoto University, Institute for Chemical Research, Assistant Professor (60303898)

坂田 完三 (2006)  京都大学, 化学研究所, 教授 (20087563)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Bun-ichi  Kyoto University, Institute for Chemical Research, Assistant Professor (50324695)
KINOSHITA Tomomi  Kagoshima Prefectural College, Dep. life and Environmental Science, Assistant Professor (20399257)
水谷 正治  京都大学, 化学研究所, 助手 (60303898)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥6,450,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥750,000)
Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2006: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsDarjeeling tea / CTC Black tea / Aroma formation / green leafhopper / thrips / Taeniothrips / Empoasca / 香気生成機構 / ヨコバイ / セカンドフラッシュ / Camellia sinensis / hotrienol / 2,6-dimethylocta-3,7-diene-2,6-diol
Research Abstract

We made the joint羊esearch contract of two遥ears collaboration with Indian Tea Research Association (TRA) and Tocklai Tea Experimental Station (TIES). Accordingly, we performed the researches as follows:
1) Investigation of the manufacturing of Darjeeling tea “Second flash"in India. At the end of May 2006, three researchers from Japan visited TTES at Tocklai, Assam and also Darjeeling in India. We, together with two researchers from TTES, visited several tea gardens in Darjeeling to see the tea manufacturing and the state of the insect擁nfestation. We also collected the tea samples at two gardens. We observed that two kinds of insects, jassid (green leaffly: Empoasca flavescens Fabr.) and thrips (Taeniothrips setiventris Bagnall), are mainly involved in the insect infestation in Darjeeling. In June 2007, Japanese researchers visited TTES. The in様aboratry breeding of the insects was performed at TTES, and the tea leaves artificially infested by the insects were prepared to analyze the effec … More ts of the infestation on the characteristic aroma formation of the tea.
2) Elucidation of the characteristic aroma formation of Darjeeling tea “Second flash"produced with the insect擁nfested leaves. The samples collected at the Darjeeling tea gardens in 2006 were analyzed by GC-MS, and it was suggested that insect infestation is involved in the aroma formation such as monoterpene alcohols and hotrienol. It was also evident from the experiments in 2007that insect infestation is mainly concerned with the characteristic aroma formation. The other experiments conducted at a Japanese tea garden demonstrated that the infestation by tea green様eafhopper (a very similar insect to jassid) is involved in the formation of 2,6-dimethylocta-3,7-diene-2,6-diol.
3) Attempts to develop a new manufacturing process of CTC black tea. We suggested to the TTES researchers that they should apply the concept in manufacturing Taiwan oolong tea to Indian black tea manufacturing to improve the quality of the flavor of CTC black tea. According to our suggestion, they tried the tea manufacturing with a pilot plant at TTES, and found that the additional processes of some mechanical damages on tea leaves during the withering may improve the quality of the tea flavor. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (30 results)

All 2008 2007 2006

All Journal Article (14 results) (of which Peer Reviewed: 2 results) Presentation (16 results)

  • [Journal Article] Biochemical Characterization of β-primeverosidase-expression with a baculovirus insect cell system and affinity purification with a primeverosylamidine as a ligand-2008

    • Author(s)
      Saino, H., Mizutani, M., Hiratake, J., Sakata, K.
    • Journal Title

      Biosci. Biotechnol. Biochem. 72

      Pages: 376-383

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 烏龍茶の香気生成機構の研究から学んだこととその食品産業への応用(その2)2008

    • Author(s)
      坂田完三
    • Journal Title

      ソフトドリンク技術資料 1

      Pages: 1-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 烏龍茶の香気生成の秘密を探る2008

    • Author(s)
      坂田完三
    • Journal Title

      きんか 60

      Pages: 1-4

    • NAID

      10004844550

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Biochemical Characterization of β-primeverosidase - expression with a baculovirus insect cell system and affinity purification with a primeverosylamidine as a ligand -2008

    • Author(s)
      Saino, H., Mizutani, M., Hiratake, J., Sakata, K.
    • Journal Title

      Biosci Biotechnol Biochem 72

      Pages: 376-383

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] What I learnt from the study on molecular mechanism of tea aroma formation during oolong tea manufacturing and its application on food industries (the second half)2008

    • Author(s)
      Sakata, K.
    • Journal Title

      Softdrink Tech. 1

      Pages: 1-14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Chemical profiling and gene expression profiling during the manufacturing process of Taiwan oolong tea "Oriental Beauty".2007

    • Author(s)
      Cho JY, Mizutani M, Shimizu B, Kinoshita T, Ogura M, Tokoro K, Lin ML, Sakata K.
    • Journal Title

      Biosci Biotechnol Biochem 71

      Pages: 1476-1486

    • NAID

      10027515791

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 烏龍茶の香気生成機構の研究から学んだこととその食品産業への応用(その1)2007

    • Author(s)
      坂田完三
    • Journal Title

      ソフトドリンク技術資料 3

      Pages: 317-334

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Chemical profiling and gene expression profiling during the manufacturing process of Taiwan oolong tea "Oriental Beauty".2007

    • Author(s)
      Cho JY, Mizutani M, Shimizu B, Kinoshita T, Ogura M, Tokoro K, Lin ML, Sakata K
    • Journal Title

      Biosoi Biotechnol Biochem 71

      Pages: 1476-1486

    • NAID

      10027515791

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] What I learnt from the study on molecular mechanism of tea aroma formation during oolong tea manufacturing and its application on food industries (the first half)2007

    • Author(s)
      Sakata, K.
    • Journal Title

      Softdrink Tech. 3

      Pages: 317-334

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] チヤ葉のジグリコシダーゼによる香気生成と食品への応用2006

    • Author(s)
      水谷正治, 坂田完三
    • Journal Title

      バイオサイエンスとインダストリー 64

      Pages: 145-150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 東方美人茶の香りの秘密2006

    • Author(s)
      木下朋美, 坂田完三
    • Journal Title

      香料 229

      Pages: 113-120

    • NAID

      40007218444

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2006 Annual Research Report
  • [Journal Article] Diglycosidase deeply concerned with tea aroma formation and its application for food industries.2006

    • Author(s)
      Mizutani, M., Sakata, K.
    • Journal Title

      Bioscience and industry 64

      Pages: 145-150

    • NAID

      10017315791

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Mysterious Aroma of Oriental Beauty2006

    • Author(s)
      Kinoshita, T., Sakata, K.
    • Journal Title

      The Kouryo 229

      Pages: 113-120

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Oolong tea manufacturing procedures and stress-responsive reactions of plants give us "Suggestions for improving flavor quality or a new processing procedure of black tea"2006

    • Author(s)
      Sakata K, Cho J-Y, Kinoshita T, Mizutani M, Shimizu B
    • Journal Title

      Proceedings of 34^<th> Tocklai Conference -Strategies for Quality -

      Pages: 9-20

    • Related Report
      2006 Annual Research Report
  • [Presentation] 烏龍茶の香気生成機構について2008

    • Author(s)
      水谷正治
    • Organizer
      羊ヶ丘植物生理化学セミナー
    • Place of Presentation
      北海道農業研究センター
    • Year and Date
      2008-03-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular mechanism of the floral aroma formation during oolng tea manufacturing2008

    • Author(s)
      Mizutani, M.
    • Organizer
      Phytochemical Seminar in Hitsujigaoka
    • Place of Presentation
      Hokkaido
    • Year and Date
      2008-03-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Understanding biotic stress regulated gene expression in Darjeeling tea for development of a new type of black tea2007

    • Author(s)
      B Gohain, T Borat, A Rahman, LP Bhuyan, G. Gurusubramanian, S. D. Ravindranath, B. Shimizn, M. Mizutani, K. Sakata, S Das, M. Hazarika
    • Organizer
      The 3rd international conference of O-CHA (tea) culture and science
    • Place of Presentation
      静岡県立大
    • Year and Date
      2007-11-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Understanding biotic stress regulated gene expression in Darjeeling tea for development of a new type of black tea.2007

    • Author(s)
      B Gohain, T Borat, A Rahman, LP Bhuyan, G. Gurusubramanian, S. D. Ravindranath, B. Shimizn, M. Mizutani, K. Sakata, S Das, M. Hazarika
    • Organizer
      The 3rd international conference of O-CHA (tea) culture and science
    • Place of Presentation
      Shizuoka
    • Year and Date
      2007-11-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular mechanism of the floral aroma formation during oolng tea manufacturing2007

    • Author(s)
      水谷正治
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      Switzerland
    • Year and Date
      2007-10-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] グルコシダーゼからジグリコシダーゼヘの分子進化2007

    • Author(s)
      齊野廣道, 水谷正治, 平竹潤, 坂田完三
    • Organizer
      第48回日本植物生理学会年会
    • Place of Presentation
      愛媛大学
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular Evolution from monoglucosidase to diglycosidase.2007

    • Author(s)
      Saino, H., Mizutani, M., Hiratake, J., Shimizu, T., Kato, H., Sakata, K.
    • Organizer
      48th Ann. Meeting of Japan Society for Plant Physiologist
    • Place of Presentation
      Ehime
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 東方美人茶の原料であるウンカ加害チャ葉から単離したCytochrorme P450によるモノテルペン水酸化の解析2007

    • Author(s)
      趙正容, 水谷正治, 木下朋美, 清水文一, 坂田完三
    • Organizer
      日本農芸化学会2007年度大会
    • Place of Presentation
      東京農大
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] チャ樹由来β-プリメベロシダーゼの二糖認識機構の解明2007

    • Author(s)
      齊野広道, 水谷正治, 平竹 潤, 清水哲也, 加藤博章, 坂田完三
    • Organizer
      日本農芸化学会2007年度大会
    • Place of Presentation
      東京農大
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] P450s in monoterpene hydroxylation from tea leaves infested by leafhoppers2007

    • Author(s)
      Cho JY, Mizutani M, Shimizu B, Kinoshita T, Sakata K.
    • Organizer
      2007 Ann. Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Elucidation of substrate recognition mechanism of b-primeverosidase.2007

    • Author(s)
      Saino, EL, Mizutani, M., Hiratake, J., Shimizu, T., Kato, H., Sakata, K.
    • Organizer
      2007 Ann. Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 東方美人茶の原料であるウンカ加害チャ葉から単離したCytochrome P450によるモノテルペン水酸化の解析2007

    • Author(s)
      趙正容、水谷正治、木下朋美、清水文一、坂田完三
    • Organizer
      日本農芸化学会2007年度大会
    • Place of Presentation
      東京農大
    • Year and Date
      2007-03-25
    • Related Report
      2007 Annual Research Report
  • [Presentation] Gene Expression profiring and Chemical Profiring during the Manufacturing Process of Formosa Oolong Tea "Oriental Beauty2006

    • Author(s)
      趙正容, 水谷正治, 清水文一, 木下朋美, 小倉, 所, 林, 坂田完三
    • Organizer
      International Conference on Biodiversity and Natural Products
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2006-07-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Distribution and Evolution of Disaccharide-specific Glycosidases(Diglycosidases)in Plants2006

    • Author(s)
      齊野廣道, 大安裕美, 藤博幸, 坂田完三, 水谷正治
    • Organizer
      International Conference on Biodiversity and Natural Products
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2006-07-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Chemical profiling and gene expression profiling during the manufacturing process of Taiwan oolong tea "Oriental Beauty".2006

    • Author(s)
      Cho JY, Mizutani M, Shimizu B, Kinoshita T, Ogura M, Tokoro K, Lin ML, Sakata K.
    • Organizer
      International Conference on Biodiversity and Natural Products
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-07-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Distribution and Evolution of Disaccharide-specific Glycosidases (Diglycosidases) in Plants2006

    • Author(s)
      Saino, H., Daiyasu, H., Toh, H., Sakata, K. Mizutani, M.
    • Organizer
      International Conference on Biodiversity and Natural Products
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-07-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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