Molecular epidemiological survey and risk evaluation of livestock animals for control of taeniasis and cycticercosi s.

• Project/Area Number: 18406008
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Tottori University
• Principal Investigator: OKAMOTO Munwhieo Tottori University, 農学部, 准教授 (70177096)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Yoshiharu 鳥取大学, 農学部, 教授 (50194410) ; ITO Akira 旭川医科大学, 医学部, 教授 (70054020) ; 迫 康仁 旭川医科大学, 医学部, 助教 (40312459)
• Co-Investigator(Renkei-kenkyūsha): SAKO Yasuhito 旭川医科大学, 医学部, 助教 (40312459)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥15,750,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥2,550,000); Fiscal Year 2008: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2007: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2006: ¥4,700,000 (Direct Cost: ¥4,700,000)
• Keywords: テニア症 / 〓虫症 / 中間宿主 / 家畜 / 分子疫学調査 / リスク評価 / 嚢虫症 / ネコ条虫 / ライブラリーワクチン / ラット / 防御抗原遺伝子 / テニア科条虫 / Nod-Scid

Research Abstract

タイ・カンチャナブリ州とインドネシア・バリ島および北スマトラで疫学調査を実施した。タイでは、ヒトの調査から無鉤条虫、有鉤条虫、Asian Taeniaの3種が同一地域に流行していることを確認した。無鉤条虫の実験感染ウシ血清およびAsian Taenia実験感染ブタ血清を用い血清診断法の開発を試みた結果、精製抗原にて良好な結果が得られた。タイの調査地で飼育されているブタの採血を行い、血清診断を実施したが、ブタの嚢虫症は確認できなかった。

Research Products

• [Journal Article] Paragonimiasis in Cameroon : molecular identification serodiagnosis and clinical manifestations. (2009)
  • Author(s): Nkouawa A ., Okamoto M ., Mabou A.K., Edingae E., Yamasaki H., Sako Y., Nakao M., Nakaya K., Blair D., Agatsuma T., Enyong P., Shibahara T., Moyou-Somo R. and Ito A
  • Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene 103 Pages: 255-261
  • NAID: 120003203341
  • Peer Reviewed
• [Journal Article] Paragonimiasis in Cameroon : molecular identification, serodiagnosis and clinical manifestations. (2009)
  • Author(s): Nkouawa A, Okamoto M, Mabou A. K., Edingae E, Yamasaki H, Sako Y, Nakao M, Nakaya K, Blair D, Agatsuma T, Enyong P, Shibahara T, Moyou-Somo R, Ito A.
  • Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene 103 Pages: 255-261
  • NAID: 120003203341
  • Peer Reviewed
• [Journal Article] Molecular and immunological diagnosis of taeniasis and cysticercosis in Asia and the Pacific (2008)
  • Author(s): Ito A., Sako Y., Nakao M., Nakaya K., Okamoto M ., Wandra T ., Dandun I .N., Anantaphruti M.T., Waikagul J., Li T. and Qiu D.
  • Journal Title: Southeast Asian Journal of Tropical Medicine and Public Health 39 supple1 Pages: 37-47
  • Peer Reviewed
• [Journal Article] Molecular and immunological diagnosis of taeniasis and cysticercosis in Asia and the Pacific. (2008)
  • Author(s): Ito A, Sako Y, Nakao M, Nakaya K, Okamoto M, Wandra T, Dandun I N, Anantaphruti M T, Waikagul J, Li T, Qiu D.
  • Journal Title: Southeast Asian Journal of Tropical Medicine and Public Health. 39supplel Pages: 37-47
  • Peer Reviewed
• [Journal Article] Sympatric Occurrence of Taenia solium, T. saginata, and T. asiatica, Thailand (2007)
  • Author(s): Anantaphruti M. T., Yamasaki H., Nakao M., Waikagul J., Watthanakulpanich D., Nuamtanong S., Maipanich W., Pubampen S., Sanguankiat S., Muennoo C., Nakaya K., Sato M. O., Sako Y., Okamoto M., and Ito A
  • Journal Title: Emerging Infectious Disease 13 Pages: 1413-1416
  • Peer Reviewed
• [Journal Article] Current Situation of Taeniasis and Cysticercosis in Indonesia (2007)
  • Author(s): Wandra T., Margono S.S., GafarM. S., Saragih J. M., Sutisna P., Sudewi A. A. R., Depary A. A., Yulfi H., Darla D. M., Okamoto M., Sato M. O., Sako Y., Nakao M., Nakaya K., Craig P. S. and Ito A
  • Journal Title: Tropical Medicine a nd Health 35 Pages: 323-328
  • NAID: 10024174694
  • Peer Reviewed
• [Journal Article] ASIAN TAENIA - SPECIES OR SUBSPECIES (2007)
  • Author(s): Okamoto M., Nakao M.,Tachi E., Sako Y., Sato O. M., Yamasaki H., Nakaya K. and Ito A
  • Journal Title: Southeast Asian Journal of Tropical Medicine and Public Health 38 supple1 Pages: 125-130
  • Peer Reviewed
• [Journal Article] Intraspecific variation of Spirometra erinaceieuropaei and phylogenetic relationship between Spirometra and Diphyllobothrium inferred from mitochondrial CO1 gene sequences (2007)
  • Author(s): Okamoto M., Iseto C., Shibahara T., Sato M. O., Wandra T., Craig P. S. and Ito A
  • Journal Title: Parasitology International 56 Pages: 235-328
  • Peer Reviewed
• [Journal Article] Intraspecific variation of Spirometra erinaceieuropaei and phylogenetic relationship between Spirometra and Diphyllobothrium inferred from mitochondrial CO1 gene sequences. (2007)
  • Author(s): Okamoto M., Iseto C., Shibahara T., Sato M. O., Wandra T., Craig P. S. and Ito A.
  • Journal Title: Parasitol Int. 56 Pages: 235-238
  • Peer Reviewed
• [Journal Article] ASIAN TAENIA-SPECIES OR SUBSPECIES?- (2007)
  • Author(s): Okamoto M., Nakao M., Tachi E., Sako S., Sato O. M., Yamasaki H., Nakaya K. and Ito A.
  • Journal Title: Southeast Asian J Trop Med Public Health. 38.Supple1 Pages: 125-130
  • Peer Reviewed
• [Journal Article] Sympatric Occurrence of Taenia solium, T. saginata, and T. asiatica, Thailand. (2007)
  • Author(s): Anantaphruti M. T., Yamasaki H., Nakao M., Waikagul J., Watt hanakulpanich D., Nuamtanong S., Maipanich W., Pubampen S., Sanguankiat S., Muennoo C., Nakaya K., Sato M. O., Sako Y., Okamoto M., and Ito A.
  • Journal Title: Emerging Infectious Disease 13 Pages: 1413-1416
  • Peer Reviewed
• [Journal Article] Current Situation of Taeniasis and Cysticercosis in Indonesia. (2007)
  • Author(s): Wandra T., Margono S. S., GafarM. S., Saragih J. M., Sutisna P., Sudewi A. A. R., Depary A. A., Yulfi H., Darla D. M., Okamoto M., Sato M. O., Sako Y., Nakao M., Nakaya K., Craig P. S. and Ito A.
  • Journal Title: Trop. Med. Health 35 Pages: 323-328
  • NAID: 10024174694
  • Peer Reviewed
• [Journal Article] Genetic Uniformity of Echinococcus multilocularis Collected from Different Intermediate Host Species in Hokkaido, Japan. (2007)
  • Author(s): Okamoto M., Oku Y., Kurosawa T., Kamiya M.
  • Journal Title: J. Vet. Med. Sci. 69・2 Pages: 159-163
  • NAID: 110006201233
• [Journal Article] Intraspecific variation of Spirometra erinaceieuropaei and phylogenetic relationship between Spirometra and Diphyllobothrium inferred from mitochondrial CO1 gene sequences. Parasitology International (2007)
  • Author(s): Okamoto M., Iseto C., Shibahara T., Sato M.O., Wandra T., Craig P.S., Ito A.
  • Journal Title: Parasitol Int. 56 (in press)
• [Journal Article] ASIAN TAENIA - SPECIES OR SUBSPECIES? - (2007)
  • Author(s): Okamoto M., Nakao M., Tachi E., Sako S., Sato O.M., Yamasaki H., Nakaya K., Ito A.
  • Journal Title: Southeast Asian J Trop Med Public Health. 38・supple (in press)
• [Journal Article] THE PRESENT SITUATION OF TAENIASIS AND CYSTICERCOSIS IN ASIA AND THE PACIFIC (2007)
  • Author(s): Ito A., Okamoto M., Wandra T., Wibisono H., Anantaphruti T.M., Waikagul J., TiLi T., Qiu D.
  • Journal Title: Southeast Asian J Trop Med Public Health. 38・supple (in press)
• [Journal Article] Genetic Uniformity of Echinococcus multilocularis Collected from Different Intermediate Host Species in Hokkaido (2006)
  • Author(s): Okamoto M ., Oku Y., Kurosawa T. and Kamiya M
  • Journal Title: Japan. Journal of Veterinary Medical Science 69 Pages: 55-60
  • Peer Reviewed
• [Journal Article] Taeniasis and cysticercosis in Bali and North Sumatra Indonesia (2006)
  • Author(s): Wandra T., D epary A. A., Sutisna P., Margono S. S., Suroso T., Okamoto M., Craig P. S. and Ito A
  • Journal Title: Parasitology International 55 sulpple1 Pages: 55-60
  • NAID: 10017322702
  • Peer Reviewed
• [Journal Article] Taeniasis and cysticercosis in Bali and North Sumatra, Indonesia. (2006)
  • Author(s): Wandra T., Depary A.A., Sutisna P., Margono S.S., Suroso T., Okamoto M., Craig P.S., Ito A.
  • Journal Title: Parasitol Int. 55・S1 Pages: 55-60
  • NAID: 10017322702
• [Presentation] Ancestral hybridization between Taenia saginata and Asian Taenia (2009)
  • Author(s): Okamoto M
  • Organizer: 43rd Annual U.S.-Japan Joint Conference on Parasitic Diseases
  • Place of Presentation: Medical Center of Japan, Tokyo, Japan
• [Presentation] Ancestral hybridization between Taenia saginata and Asian Taenia. (2009)
  • Author(s): Okamoto M.
  • Organizer: 43rd Annual U. S. -Japan Joint Conference on Parasitic Diseases
  • Place of Presentation: Medical Center of Japan, Tokyo, Japan
• [Presentation] Evidence of ancestral hybridization between Taenia saginata and Asian Taenia (2008)
  • Author(s): Okamoto M
  • Organizer: XVIIth International Congress of Tropical Medicine and Malaria
  • Place of Presentation: International Convention Center Jeju, Korea
• [Presentation] Evidence of ancestral hybridization between Taenia saginata and Asian Taenia. (2008)
  • Author(s): Okamoto M.
  • Organizer: XVIIth International Congress of Tropical Medicine and Malaria
  • Place of Presentation: International Convention Center Jeju, Korea
• [Presentation] Is Asian Taenia Species or Subspecies? (2007)
  • Author(s): Okamoto M, Nakao M, Anataphruti M. T, Tachi E., Sako Y., Sato M. O., Yamasaki H., Nakaya N., Waikagul J. and Ito A.
  • Organizer: 21th Pacific Science Congress
  • Place of Presentation: Ginowan, Okinawa
  • Year and Date: 2007-06-14
• [Presentation] Is Asian Taenia Species or Subspecies? (2007)
  • Author(s): Okamoto M., Nakao M., Anataphruti M.T., Tachi E., Sako Y., Sato M. O., Yamasaki H., Nakaya N., Waikagul J. and Ito A
  • Organizer: 21th Pacific Science Congress
  • Place of Presentation: Ginowan, Okinawa, Japan
• [Presentation] Asian Taenia(タイワンテニア)は独立種か、それとも亜種か (2007)
  • Author(s): 岡本宗裕、中尾稔、舘英子、迫康仁、Marcello O. Sato、山崎浩、中谷和宏、伊藤亮
  • Organizer: 第76回日本寄生虫学会大会
  • Place of Presentation: 大阪
• [Presentation] ASIAN TAENIA - SPECIES OR SUBSPECIES? (2006)
  • Author(s): Okamoto M, Nakao M, Tachi E., Sako Y., Sato M. O., Yamasaki H., Nakaya N. and Ito A
  • Organizer: FBPZ 2006
  • Place of Presentation: Bangkok, Thailand
• [Book] Taenia (2009)
  • Author(s): Ito A , Nakao M, Sako Y , Nakaya K, Yanagida T, Okamoto M
  • Publisher: Molecular Detection of Foodborne Pathogens Chapter 62
• [Book] Molecular Detection of Foodborne Pathogens Chapter 62. Taenia (2009)
  • Author(s): Ito A, Nakao M, Sako Y, Nakaya K, Yanagida T, Okamoto M.
  • Publisher: Taylor & Francis Group, London
• [Book] Purification of nucleic acids from helminthes (2008)
  • Author(s): Okamoto M, Ito A
  • Publisher: Handbook of Nucleic Acid Purification Chapter 13
• [Book] Handbook of Nucleic Acid Purification Chapter 13. Purification of nucleic acids from helminthes (2008)
  • Author(s): Okamoto M, Ito A.
  • Publisher: Taylor & Francis Group, London