Structure and Function of Weak Interaction in Blue Copper Protein

• Project/Area Number: 18550147
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Ibaraki University
• Principal Investigator: KOHZUMA Takamitsu Ibaraki University, 理工学研究科, 教授 (50215183)
• Co-Investigator(Kenkyū-buntansha): 鳥飼 直也 高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (70300671)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,490,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥690,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: ブルー銅タンパク質 / 弱い相互作用 / 電子移動反応 / シュウドアズリン / X線吸収スペクトル / アルカリ構造転 / 電子伝達タンパク質 / 弱い相互と用 / 亜硝酸還元酵素

Research Abstract

ブルー銅タンパク質に配位するヒスチジンへの弱い相互作用の様式が変化する事によって、活性中心のCu(II)の電子状態が変化し、酸化還元電位などが顕著に変化することが見いだされた。この結果は、タンパク質複合体形成などにおける弱い相互作用が生理学的に意味の有る範囲において顕著に反応性を微調整することを示唆するものである。

Research Products

• [Journal Article] Alkaline Transition of Pseudoazurin Met16X Mutant Proteins : Protein Stability Influenced by the Substitution of Met16 in the Second Sphere Coordination (2008)
  • Author(s): Rehab F. Abdelhamid, Yuji Obara, Takamitsu Kohzuma
  • Journal Title: J. Inorg. Biochem. 102 Pages: 1373-1379
  • Peer Reviewed
• [Journal Article] π-Interaction Tuning of the Active Site Properties of Metalloproteins (2008)
  • Author(s): Sachiko Yanagisawa
  • Journal Title: J. Am. Chem. Soc
  • Peer Reviewed
• [Journal Article] A new titration system of a novel split-type superconducting magnet NMR spectrometer (2008)
  • Author(s): Isao Kitagawa
  • Journal Title: REVIEW OF SCIENTIFIC INSTRUMENTS 79 Pages: 123109-123109
  • Peer Reviewed
• [Journal Article] Alkaline transition of pseudoazurin Met16X mutant proteins: Protein stability influenced by the substitution of Met16 in the second sphere coordination (2008)
  • Author(s): Rehab F. Abdelhamid
  • Journal Title: J. Inorg. Biochem. 102 Pages: 1373-1379
  • Peer Reviewed
• [Journal Article] π-π Interaction between Aromatic Ring and Copper Coordinated His81 Imidazole Regulates the Blue Copper Active Site Structure (2007)
  • Author(s): Rehab F. Abdelhamid, Yuji Obara, Yoshiko Uchida, Takamitsu Kohzuma, David M. Dooley, Doreen E. Brown, and Hiroshi Hori
  • Journal Title: J. Biol. Inorg. Chem. 12 Pages: 165-173
  • Peer Reviewed
• [Journal Article] π-π Interaction between Aromatic Ring and Copper Coordinated His81 Imidazole Regulates the Blue Copper Active Site Structure (2007)
  • Author(s): Regab F.Abdelhamid 他6名
  • Journal Title: J. Biol. Inorg. Chem. 12 Pages: 165-165
• [Journal Article] Intersite Structural Rearrangement of the Blue Copper Site Induced by Substrate Binding : Spectroscopic Studies of Copper-Containing Nitrite Reductase from Alcaligenesxylosoxidans NCIMB 11015 (2006)
  • Author(s): Takamitsu Kohzuma 他5名
  • Journal Title: Inorg. Chem. 45 Pages: 8474-8474
• [Journal Article] p-Interaction Tuning of the Active Site Properties of Metalloproteins
  • Author(s): Sachiko Yanagisawa, Peter B. Crowley, Susan J. Firbank, Anne T. Lawler, David M. Hunter, William McFarlane, Chan Li, Takamitsu Kohzuma, Mark J. Banfield, and Christopher Dennison
  • Journal Title: J. Am. Chem. Soc.
  • Peer Reviewed
• [Presentation] 脱窒菌Alcaligenes xylosoxidans NCIMB11015由来ブルー銅タンパク質アズリンIとアズリンIIの二量体形成に関する研究 (2009)
  • Author(s): 木村宗太郎、田中秀樹、北川功、岡田道哉、高妻孝光
  • Organizer: 日本化学会第89回春季年会
  • Place of Presentation: 日本大学・船橋
• [Presentation] ブルー銅タンパク質シュウドアズリンにおける静電的相互作用の効果 (2009)
  • Author(s): 小原裕二
  • Organizer: 第89回春季日本化学会年会
  • Place of Presentation: 日本大学・船橋
• [Presentation] 脱窒菌Alcalgenes xylosoxidans NCIMB11015由来ブルー銅タンパク質アズリンIとアズリンIIの紫外共鳴ラマンスペクトルの測定 (2008)
  • Author(s): 木村宗太郎、田中秀樹、北川功、岡田道哉、高妻孝光
  • Organizer: 日本化学会関東支部茨城地区研究交流会
  • Place of Presentation: ワークプラザ勝田・勝田市
  • Year and Date: 2008-11-21
• [Presentation] ブルー銅タンパク質シュウドアズリンにおける静電的相互作用の効果(ポスター奨励賞受賞) (2008)
  • Author(s): 仁平裕子
  • Organizer: 日本化学会関東支部茨城地区研究交流会
  • Place of Presentation: ワークプラザ勝田・勝田
  • Year and Date: 2008-11-21
• [Presentation] Perturbation of the Protein Electronic Structure through Weak Chemical Interaction (2008)
  • Author(s): T. Kohzuma
  • Organizer: 6th China-Japan Crossover Science Symposiumu
  • Place of Presentation: 鄭州大学・中国
  • Year and Date: 2008-10-24
• [Presentation] The role of 2nd coordination sphere in a blue copper protein, pseudoazurin (2008)
  • Author(s): Takamitsu Kohzuma, Rehab F. Abdelhamid, Yuji Obara
  • Organizer: 9th European Conference of Biological Chemistry, Broclow, Poland(招待講演)
  • Place of Presentation: Poland
• [Presentation] Perturbation of the Protein Electronic Structure through Weak Chemical Interaction (2008)
  • Author(s): Takamitsu Kohzuma
  • Organizer: 6th China-Japan Crossover Science Symposium(招待講演)
  • Place of Presentation: Zhengzhou, China
• [Presentation] The role of 2nd coordination sphere in a blue copper protein, pseudoazurin (Keynote Lecture) (2008)
  • Author(s): T. Kohzuma, R. F. Abdelhamid, Y. Obara
  • Organizer: 9th European Biological Inorganic Chemistry Conference
  • Place of Presentation: ブラツワフ大学・ポーランド
• [Presentation] Effect of Weak Interaction on the Electronic Structure and Electrochemical Properties of Pseudoazurin Met 16X mutants (2008)
  • Author(s): Yuji Obara
  • Organizer: 9th European Biological Inorganic Chemistry Conference
  • Place of Presentation: ブラツワフ大学・ポーランド
• [Presentation] UV Resonance Raman Spectroscopic Studies of Azurin I and Azurin II from Alcaligenes xylosoxidans NCIMB 11015 (2008)
  • Author(s): S. Kimura, R. F. Abdelhamid, T. Kohzuma
  • Organizer: 9th European Biological Inorganic Chemistry Conference
  • Place of Presentation: ブラツワフ大学・ポーランド
• [Presentation] 脱窒菌Alcaligenes xylosoxidans NCIMB11015由来ブルー銅タンパク質アズリンIとアズリンIIの紫外共鳴ラマンスペクトル (2008)
  • Author(s): 木村宗太郎、田中秀樹、北川功、岡田道哉、高妻孝光
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド・神戸
• [Presentation] Effect of Weak Interaction on the Electronic Structure and Ele ctrochemical Properties of Pseudoazurin Metl6X mutants (2008)
  • Author(s): 小原裕二
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡国際会議場・福岡
• [Presentation] ブルー銅タンパク質シュウドアズリンにおける静電的相互作用の効果 (2008)
  • Author(s): 小原裕二
  • Organizer: 第58回錯体化学討論会
  • Place of Presentation: 金沢大学・金沢
• [Presentation] Spectroscopic and Electrochemical Studies of Pseudoazurin Met16X Variants (2007)
  • Author(s): 小原裕二, 他3名
  • Organizer: 第57回錯体化学討論会
  • Place of Presentation: 名古屋工業大学
  • Year and Date: 2007-09-25
• [Presentation] Weak Interaction Regulates the Active Site Structure of a Blue Copper Protein, Pseudoazurin (2007)
  • Author(s): Takamitsu Kohzuma
  • Organizer: 第13回国際生物無機化学会議
  • Place of Presentation: ウィーン大学
  • Year and Date: 2007-07-16
• [Presentation] Spectroscopic and Electrochemical Studies of Pseudoazurin M16X Variants (2007)
  • Author(s): Yuji Obara, 他3名
  • Organizer: 第13回国際生物無機化学会議
  • Place of Presentation: ウィーン大学
  • Year and Date: 2007-07-16
• [Presentation] UV Resonance Raman Spectroscopic Studies of Cytochrome c'from Alcaligenes xylosoxidans NCIMB 11015 (2007)
  • Author(s): Naoko Shiono and Takamitsu Kohzuma
  • Organizer: 第13回国際生物無機化学会議
  • Place of Presentation: ウィーン大学
  • Year and Date: 2007-07-16
• [Presentation] Resonance Raman Spectroscopic Studies on the Alkaline Transition of a Blue Copper Protein, Pseudoazurin and Its M16X Variants (2007)
  • Author(s): Rehab F. Abdelhamid and Takamitsu Kohzuma
  • Organizer: 第13回国際生物無機化学会議
  • Place of Presentation: ウィーン大学
  • Year and Date: 2007-07-16
• [Presentation] UV Resonance Raman Spectroscopic Studies of Human Hematopoietic Prostaglandin D_2 Synthase, International Symposium on Biological Application of Vibrational Spectroscopy (2007)
  • Author(s): Takamitsu Kohzuma
  • Organizer: International Symposium on Biological Application of Vibrational Spectroscopy, Center for Advanced Science and Technology, Hyogo(招待講演)
  • Place of Presentation: Hyogo, Japan
• [Book] 元素111の新知識第2版 (2009)
  • Author(s): 桜井弘、荒野泰、上山憲一、小谷明、高妻孝光、佐治英郎、鈴木晋一郎、寺嶋孝仁、中山祐正、根矢三郎、廣田俊、藤井敏司、吉村哲彦
  • Total Pages: 461
  • Publisher: 講談社
• [Book] 元素111の新知識第2版 (2009)
  • Author(s): 桜井弘
  • Total Pages: 431
  • Publisher: 講談社
• [Remarks]
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• [Remarks] 茨城大学研究者情報総覧
  • URL: http://info.ibaraki.ac.jp/scripts/websearch/index.htm