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Symbiosis of Frenulata with chemoautotrophic bacteria : clarification of bacterial control by host cells

Research Project

Project/Area Number 18570057
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionKanazawa University

Principal Investigator

SASAYAMA Yuichi  Kanazawa University, 環日本海域環境研究センター, 教授 (30018999)

Co-Investigator(Kenkyū-buntansha) FUKUMORI Yoshihiro  金沢大学, 自然システム学系, 教授 (60135655)
KANEMORI Masaaki  金沢大学, 自然システム学系, 講師 (20324064)
MATSUNO Akira  島根大学, 生物資源科学部, 教授 (60032629)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2006: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywords動物形態 / ヒゲムシ / 化学合成細菌 / 共生 / バクテリオサイト / 共生細菌 / イオウ酸化細菌 / α-グルコシダーゼ活性 / 細胞内分布 / 毛細血管 / Siboglinidae / マシコヒゲムシ / 16S rDNA / in situ hybridization / 硫化水素 / 栄養体
Research Abstract

口も消化管も無いヒゲムシにおいて栄養物を提供してくれる化学合成細菌を、共生させているバクテリオサイトと呼ばれる細胞において、それらの細菌が細胞内でどの場所を占めているかを調べた。その結果、それらの細菌のほとんどは、バクテリオサイトと、その外周にある栄養貯蔵細胞層との間に存在する毛細血管に向いて存在していることが明らかになった。これは細菌の生産物を栄養貯蔵細胞全体に行き渡らすに都合が良いと判断された。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report

Research Products

(33 results)

All 2008 2007 2006 Other

All Journal Article (21 results) (of which Peer Reviewed: 16 results) Presentation (9 results) Remarks (3 results)

  • [Journal Article] Structural basis for the heterotropic and homotropic interactions of invertebrate giant hemoglobin2008

    • Author(s)
      Numoto N, Nakagawa T, Kita A, Sasayama Y, Fukumori Y, Miki K
    • Journal Title

      Biochemistry 47

      Pages: 11231-11238

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct evidence that extracellular giant hemoglobin is produced in chloragogen tissues in a beard worm, Oligobrachia mashikoi (Frenulata, Siboglinidae, Annelida)2008

    • Author(s)
      Nakahama S, Nakagawa T, Kanemori M, Fukumori Y, Sasayama Y
    • Journal Title

      Zool. Sci. 25

      Pages: 1247-1252

    • NAID

      120001170071

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Alpha-glucosidase-like activity detected in a siboglinid polychaete, Oligobrachia mashikoi2008

    • Author(s)
      Koizumi T, Sasayama Y
    • Journal Title

      Zool. Sci 25

      Pages: 364-271

    • NAID

      120001130208

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure of the partially unliganded met state of 400 kDa hemoglobin : Insights into ligand-induced structural changes of giant hemoglobins2008

    • Author(s)
      Numoto N, Nakagawa T, Sasayama Y, Fukumori Y, Miki K
    • Journal Title

      Proteins 73

      Pages: 111-125

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distribution and population of free-living cells related to endosymbiont A harbored in Oligobrachia mashikoi (a siboglinid polycheate) inhabiting Tsukumo Bay2008

    • Author(s)
      Aida M, Kanemori M, Kubota N, Matada M, Sasayama Y, Fukumori Y
    • Journal Title

      Microbes Environ 23

      Pages: 81-88

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for the heterotropic and homotropic interactions of invertebrate giant hemoglobin.2008

    • Author(s)
      Numoto N, Nakagawa T, Kita A, Sasayama Y, Fukumori Y, Miki K
    • Journal Title

      Biochemistry 47

      Pages: 11231-11238

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct evidence that extracellular giant hemoglobin is produced in chloragogen tissues in a beard worm, Oligobrtichia mashikoi (Frenulata, Siboglinidae, Annelida).2008

    • Author(s)
      Nakahama S, Nakagawa T, Karieinori, M, fukumori Y Sasayama Y
    • Journal Title

      Zoological Science 25

      Pages: 1247-1252

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alpha-glucosidase-like activity detected in a siboglinid polychaete, Oligobnichia mashikoi.2008

    • Author(s)
      Koizumi T, Sasayama Y
    • Journal Title

      Zoological Science 25

      Pages: 364-371

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] On the alpha-glucosidase-like activity detected in a siboglinid polychaeta,Oligobrachia mashikoi2008

    • Author(s)
      Koizumi T and Sasayama Y
    • Journal Title

      Zoological Science (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution and population of free-living cells related to endosymbiont harbored in Oligobrachia mashlkoi(a siboglinid polychaete)inhabiting Tsukumo bay2008

    • Author(s)
      Aida M, Kanemori M, Kubota N Matada M, Sasayama Y, Fukumori Y
    • Journal Title

      Microbes Environment (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between the lifestyle of a siboglinid (Pogonophora) polychaete, Oligobrachia mashikoi, and the total sulfide and nitrogen levels in its habitat2007

    • Author(s)
      Sasayama Y, Higashide Y, Sakai M, Matada M, Fukumori Y
    • Journal Title

      Zool. Sci 24

      Pages: 131-136

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Actual distribution of bacteriocytes in the trophosome of a beard worm (Oligobrachia mashikoi, Siboglinidae, Annelida) : Clarification using whole-mount in situ hybridization2007

    • Author(s)
      Deguchi M, Kubota N, Matsuno A, Kanemori M, Fukumori Y, Sasayama Y
    • Journal Title

      Acta Zool 88

      Pages: 129-135

    • NAID

      120001135382

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of endosymbionts in Oligobrachia mashikoi (Siboglinidae, Annelida)2007

    • Author(s)
      Kubota N, Kanemori M, Sasayama Y, Aida M, Fukumori Y
    • Journal Title

      Microbes Environ 22

      Pages: 136-144

    • NAID

      110006290376

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oxygenation properties of extracellular giant hemoglobin from Oligobrachia mashikoi2007

    • Author(s)
      Aki Y, Nakagawa T, Nagai M, Sasayama Y, Fukumori Y, Imai K
    • Journal Title

      BBRC 360

      Pages: 673-678

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oxygenation properties of extracellular giant hemoglobin from Oligobrachia mashikoi2007

    • Author(s)
      Aki Y, Nakagawa T, Nagai M, Sasayama Y, Fukumori Y, lmai K
    • Journal Title

      Biochem.Biophys.Res.Commun 360

      Pages: 673-678

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between the lifestyle of a Siboglinid (pogonophoran) Pplychaete, Oligobrachia mashikoi, and the total sulfide and nitrogen levels in its habitat2007

    • Author(s)
      Yuichi Sasayama et al.
    • Journal Title

      Zoological Science (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Actual distribution of bacteriocytes in the trophosome of a beard worm (Oligobrachia mashikoi, Pogonophora) : Clarification using whole-mount in situ hybridization2007

    • Author(s)
      Deguchi Mariko et al.
    • Journal Title

      Acta Zologica (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of endosymbionts in Oligobrachia mashikoi, Siboglinidae Annelida2007

    • Author(s)
      Norihiro Kubota et al.
    • Journal Title

      Microbes and Environments (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Lipid composition of the trophosome in the beard worm, Oligobrachia mashikoi (Pogonophora)2006

    • Author(s)
      Mita M, Deguchi M, Sasayama Y
    • Journal Title

      J. Mar. Biol. Ass. U.K 86

      Pages: 283-286

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A discussion of the morphology of the central lumen in the trophosome of a Frenulata (beard worm), Oligobrachia mashikoi, Siboglinidae, Annelida

    • Author(s)
      Matsuno A, Sasayama Y
    • Journal Title

      Rep. Noto Mar. Cent 14(in press)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Detection of sulfur using an X-ray analytical microscope from the trophosome of a beard worm, Oligobrachia mashikoi, Frenulata, Siboglinidae

    • Author(s)
      Sasayama Y, Fukumori Y, Nakabayashi H, Shimizu N
    • Journal Title

      Nihonkai Kenkyu (in press)

    • Related Report
      2008 Final Research Report
  • [Presentation] 環形動物門マシコヒゲムシに存在するα-グルコシダーゼ様活性を示す蛋白の生化学的研究2007

    • Author(s)
      小泉隆、山田哲也、笹山雄一
    • Organizer
      第78回日本動物学会
    • Place of Presentation
      弘前
    • Year and Date
      2007-09-20
    • Related Report
      2008 Final Research Report
  • [Presentation] 環形動物門マシコヒゲムシに存在するα-グルほシダーゼ様活性を示す蛋白の生化学的研究2007

    • Author(s)
      小泉隆、山田哲也、笹山雄一
    • Organizer
      日本動物学套第78回大会(弘前)
    • Place of Presentation
      弘前大学文京町キャンパス
    • Year and Date
      2007-09-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] Search for the genes related to chemoautotrophic sulfur oxidation in the genome of Oligobrachia mashikoi endosymbiont2007

    • Author(s)
      Aida M, Kubota N, Asada R, Kodani T, Sasayama Y, Kanemori M, Fukumori Y
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      愛媛
    • Year and Date
      2007-09-15
    • Related Report
      2008 Final Research Report
  • [Presentation] 環形動物多毛類のマシコヒゲムシの栄養体の生化学的研究2006

    • Author(s)
      小泉隆、笹山雄一
    • Organizer
      第77回日本動物学会
    • Place of Presentation
      島根
    • Year and Date
      2006-09-22
    • Related Report
      2008 Final Research Report
  • [Presentation] マシコヒゲムシのバクテリオサイトにおける熱ショック蛋白の検出の試み2006

    • Author(s)
      国田新平、金森正明、福森義宏、笹山雄一
    • Organizer
      第77回日本動物学会
    • Place of Presentation
      島根
    • Year and Date
      2006-09-22
    • Related Report
      2008 Final Research Report
  • [Presentation] マシコヒゲムシの栄養体における抗アポト-シス関連酵素抗体による免疫組織学的研究2006

    • Author(s)
      浅田光子、角明子、笹山雄一、松野あきら
    • Organizer
      第77回日本動物学会
    • Place of Presentation
      島根
    • Year and Date
      2006-09-22
    • Related Report
      2008 Final Research Report
  • [Presentation] マシコヒゲムシの巨大ヘモグロビン : in situ hybridization 法を用いた産生部位の研究2006

    • Author(s)
      中浜重之、中川太郎、金森正明、福森義宏、笹山雄一
    • Organizer
      第77回日本動物学会
    • Place of Presentation
      島根
    • Year and Date
      2006-09-22
    • Related Report
      2008 Final Research Report
  • [Presentation] マシコヒゲムシのcDNAライブラリーの作成と発現遺伝子の解析2006

    • Author(s)
      榎本あきら、岡田アキ、福森義宏、笹山雄一、山口和男
    • Organizer
      第77回日本動物学会
    • Place of Presentation
      島根
    • Year and Date
      2006-09-22
    • Related Report
      2008 Final Research Report
  • [Presentation] マシコヒゲムシ(環形動物多毛類)の単離されたバクテリオサイトにおけるバクテリアと細胞骨格2006

    • Author(s)
      山田哲也、笹山雄一
    • Organizer
      日本動物学会中部支部大会
    • Place of Presentation
      名古屋
    • Year and Date
      2006-07-30
    • Related Report
      2008 Final Research Report
  • [Remarks] 笹山雄一・福森義宏・東出幸吾 「生きたヒゲムシ撮影 : 進化の謎を解く鍵に、氷河期から現代に適応」 北國新聞 2007年4月10日カラ-掲載

    • Related Report
      2008 Final Research Report
  • [Remarks] 笹山雄一・福森義宏 「ヒゲムシ生態撮った : 世界初 九十九湾で金大教授ら」 北陸中日新聞 2007年4月10日カラ-掲載

    • Related Report
      2008 Final Research Report
  • [Remarks] 笹山雄一・福森義宏 「九十九湾のマシコヒゲムシ : 水深25m撮影成功、金沢大、世界初」 読売新聞 2007年 4月11日

    • Related Report
      2008 Final Research Report

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Published: 2006-03-31   Modified: 2016-04-21  

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