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Understanding of the photoreceptor cell function in vision by molecular replacement (knock-in) methods

Research Project

Project/Area Number 18570152
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionKyoto University

Principal Investigator

IMAI Hiroo  Kyoto University, Primate Research Institute, Associate Professor (60314176)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,860,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsrhodopsin / knock-in / vision / ES cell / retina / protein / G-protein / cone / 光
Research Abstract

Most vertebrate retinas contain two types of photoreceptor cells, rods and c ones, which are responsible for twilight and daylight vision, respectively. The rod and cone visual pigments exhibit different molecular properties before and after light absorption. That is, cone pigments show rapid formation and decay of G protein-activating states upon absorption of light, while they show high susceptibility to hydroxylamine in the dark. The amino acid residues responsible for the former difference have been identified ; We have identified the site and demonstrated the effect of the site using knock-in mice. However, the residue(s) for the latter difference have not been identified due to the vast divergence of amino acid sequence between rod and cone pigments. We have cloned and expressed primate rhodopsin and compared its susceptibility to hydroxylamine with bovine rhodopsin. As a result, primate rhodopsin showed significantly higher susceptibility than bovine rhodopsin. We also designed and expressed site directed mutants of primate and bovine rhodopsin to identify the amino acid residue(s) responsible for the difference. Some mutants in which the hydrophobic residues in the transmembrane regions are changed showed altered susceptibility to hydroxylamine. These results suggest that the susceptibility to hydroxylamine is regulated by the hydrophobic interact ions in transmembrane regions. Because the residues in cone pigments are different from bovine rhodopsin, these findings would give a due to elucidating the mechanism for the difference in the susceptibility to hydroxylamine between rod and cone visual pigments.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (44 results)

All 2007 2006 Other

All Journal Article (20 results) (of which Peer Reviewed: 8 results) Presentation (23 results) Book (1 results)

  • [Journal Article] Molecular properties of rhodopsin and rod function.2007

    • Author(s)
      H. Imai
    • Journal Title

      J. Biol. Chem. 282

      Pages: 6677-6684

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Stage-specific association of apolipoprotein a-I and e in developing mouse retina.2007

    • Author(s)
      S. Kurumada
    • Journal Title

      Invest Ophthalmol Vis Sci. 48

      Pages: 1815-1823

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Sexual difference of color sense in a lycaenid butterfly, Narathura japonica2007

    • Author(s)
      M. Imafuku
    • Journal Title

      Zool. Sci. 24

      Pages: 611-613

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Photoisomerization Efficiency in UV-Absorbing Visual Pigments: Protein-Directed Isomerization of an Unprotonated Retinal Schiff Base.2007

    • Author(s)
      K. Tsutsui
    • Journal Title

      Biochemistry 46

      Pages: 6437-6445

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Physiological properties of rod photoreceptor cells in green-sensitivecone pigment knock-in mice.2007

    • Author(s)
      K. Sakurai
    • Journal Title

      J. Gen. Physiol. 130

      Pages: 21-40

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Chondroitinase ABC treatment enhances synaptogenesis between transplant and host neurons in model of retinal degeneration.2007

    • Author(s)
      T. Suzuki
    • Journal Title

      Cell Transplant. 16

      Pages: 493-503

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Molecular properties of rhodopsin and rod function2007

    • Author(s)
      H., Imai, et. al.
    • Journal Title

      J. Biol. Chem 282-9

      Pages: 6677-6684

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Stage-specific association of apolipoprotein a-I and e in developing mouse retina2007

    • Author(s)
      S., Kurumada, et. al.
    • Journal Title

      Invest Ophthalmol Vis Sci 48-4

      Pages: 1815-1823

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Sexual, difference of color sense in a lycaenid butterfly, Narathura japonica2007

    • Author(s)
      M., Imafuku, et. al.
    • Journal Title

      Zool. Sci 24-6

      Pages: 611-613

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Photoisomerization Efficiency in UV-Absorbing Visual Pigments : Protein-Directed Isomerization of an Unprotonated Retinal Schiff Base2007

    • Author(s)
      K., Tsutsuiet, et. al.
    • Journal Title

      Biochemistry 46-21

      Pages: 6437-6445

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Physiological properties of rod photoreceptor cells in green-sensitive cone pigment knock-in mice2007

    • Author(s)
      K., Sakurai, et. al.
    • Journal Title

      J. Gen. Physiol 130-1

      Pages: 21-40

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Chondroitinase ABC treatment enhances synaptogenesis between transplant and host neurons in model of retinal degeneration2007

    • Author(s)
      T., Suzuki, et. al.
    • Journal Title

      Cell Transplant 16-5

      Pages: 493-503

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Physiological properties of rod photoreceptor cells in green-sensitive cone pigment knock-in mice.2007

    • Author(s)
      K. Sakurai
    • Journal Title

      J. Gen. Physiol 130

      Pages: 21-40

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular properties of rhodopsin and rod function.2007

    • Author(s)
      H.Imai
    • Journal Title

      J. Biol. Chem. 282

      Pages: 6677-6684

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Stage-specific association of apolipoprotein a-I and e in developing mouse retina.2007

    • Author(s)
      S.Kurumada
    • Journal Title

      Invest Ophthalmol Vis Sci. 48

      Pages: 1815-1823

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Divergent Selection on Opsins Drives Incipient Speciation in Lake Victoria Cichlids.2006

    • Author(s)
      Y. Terai
    • Journal Title

      PLoS Biology 4

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Divergent Selection on Opsins Drives Incipient Speciation in Lake Victoria Cichlids2006

    • Author(s)
      Y., Terai, et. al.
    • Journal Title

      PLoS Biol 433

      Pages: 4-12

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Divergent Selection on Opsins Drives Incipient Speciation in Lake Victoria Cichlids.2006

    • Author(s)
      Y.Terai
    • Journal Title

      PLoS Biol. 4

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Sexual difference of color sense in a lycaenid butterfly, Narathura Japonica

    • Author(s)
      M.Imafuku
    • Journal Title

      Zool. Sci. (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Photoisomerization Erriciency in UV-Absorbing visual Pigments. Protein-Directed Isomerization of an Unprotonated Retinal Schiff Base

    • Author(s)
      K.Tsutsui
    • Journal Title

      Biochemistry (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Presentation] 霊長類ロドプシンから見た光受容蛋白質のヒドロキシルアミン安定性の違い2007

    • Author(s)
      今井啓雄
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Difference in hydroxylamine susceptibility between primate and bovine rhodopsins2007

    • Author(s)
      H., Imai
    • Organizer
      45th Annual Meeting of the Biophysical Society of Japan, Yokohama
    • Year and Date
      2007-12-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 霊長類ロドプシンから見た光受容蛋白質のヒドロキシルアミン安定性の違い2007

    • Author(s)
      今井啓雄
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜市
    • Year and Date
      2007-12-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] グッピー視細胞における長波長域感受性多型の遺伝子基盤.2007

    • Author(s)
      笠木 聡
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 部位特異的変異ロドプシンノックインマウス視細胞中におけるロドプシンの性質と生理応答.2007

    • Author(s)
      今井啓雄
    • Organizer
      視覚フォーラム第11回大会
    • Place of Presentation
      岡崎
    • Year and Date
      2007-10-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular properties of rhodopsin and rod function in the retina of rhodopsin knock-in mice2007

    • Author(s)
      H., Imai
    • Organizer
      Vision Forum
    • Year and Date
      2007-10-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 部位特異的変異ロドプシンノックインマウス視細胞中におけるロドプシンの性質と生理応答2007

    • Author(s)
      今井啓雄
    • Organizer
      視覚フォーラム第11回大会
    • Place of Presentation
      岡崎市
    • Year and Date
      2007-10-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] 紫外光感受性視物質における高効率な光異性化の機構.2007

    • Author(s)
      筒井 圭
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前
    • Year and Date
      2007-09-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 受容体から見たノックインマウスと霊長類の視覚.2007

    • Author(s)
      今井啓雄
    • Organizer
      霊長類研究所共同利用研究会「霊長類ゲノムと脳・感覚研究の最前線」
    • Place of Presentation
      犬山
    • Year and Date
      2007-09-06
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Photoisomerization efficiency in UV-absorbing visual pigments2006

    • Author(s)
      K. Tsutsui
    • Organizer
      The 3rd Asia and Oceania Conference on Photobiology
    • Place of Presentation
      Beijing, China
    • Year and Date
      2006-11-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Photoisomerization efficiency in VN-absorbing visual pigments2006

    • Author(s)
      K., Tsutsui
    • Organizer
      The 3rd Asia and Oceania Conference on Photobiology, Beijing
    • Year and Date
      2006-11-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulation of the photoisomerization efficiency in visible and UV-abso rbing visual pigments.2006

    • Author(s)
      K. Tsutsui
    • Organizer
      5th Asian Biophysics Symposium & 44th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Okinawa
    • Year and Date
      2006-11-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Insights into the role of the covalent bond between rhodopsin and the chromophore.2006

    • Author(s)
      T. H. Matsuyama
    • Organizer
      5th Asian Biophysics Symposium & 44th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Okinawa
    • Year and Date
      2006-11-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulation of the photoisomerization efficiency in visible and UV-absorbing visual pigments2006

    • Author(s)
      K., Tsutsui
    • Organizer
      5th Asian Biophysics Symposium & 44th Annual Meeting of the Biophysical Society of Japan, Okinawa
    • Year and Date
      2006-11-13
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Insights into the role of the covalent bond between rhodopsin and the chromophore2006

    • Author(s)
      T. H., Matsuyama
    • Organizer
      5th Asian Biophysics Symposium & 44th Annual Meeting of the Biophysical Society of Japan, Okinawa
    • Year and Date
      2006-11-13
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 光受容タンパク質:ニワトリ、ノックインマウス、霊長類.2006

    • Author(s)
      今井啓雄
    • Organizer
      第36回ホミニゼーション研究会 「見る、聞く、話すの進化」
    • Place of Presentation
      犬山
    • Year and Date
      2006-11-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 視物質部位特異的変異体ノックインマウスの作製.2006

    • Author(s)
      今井啓雄
    • Organizer
      SCS視覚研究会
    • Place of Presentation
      犬山
    • Year and Date
      2006-06-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mutational Analysis of the Chloride Binding Site in Long-Wavelength-Sensitive Cone Visual Pigment.2006

    • Author(s)
      T. Morizumi
    • Organizer
      12th International Conference on Retinal Proteins
    • Place of Presentation
      Awaji
    • Year and Date
      2006-06-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Photoresponses of rod photoreceptor cells in cone pigment knock-in mice.2006

    • Author(s)
      K. Sakurai
    • Organizer
      12th International Conference on Retinal Proteins
    • Place of Presentation
      Awaji
    • Year and Date
      2006-06-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of Schiff Base Protonation on the Quantum Yield of Visual Pigments.2006

    • Author(s)
      K. Tsutsui
    • Organizer
      12th International Conference on Retinal Proteins
    • Place of Presentation
      Awaji
    • Year and Date
      2006-06-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mutational analysis of the chloride binding site in long-wavelength-sensitive cone visual pigment2006

    • Author(s)
      T., Morizumi
    • Organizer
      126th International Conference on Retinal Proteins, Awaji
    • Year and Date
      2006-06-08
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Photoresponses of rod photoreceptor cells in cone pigment knock-in mice2006

    • Author(s)
      K., Sakurai
    • Organizer
      12th International Conference on Retinal Proteins, Awaji
    • Year and Date
      2006-06-08
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of Schiff Base Protonation on the Quantum Yield of Visual Pigments2006

    • Author(s)
      K., Tsutsui
    • Organizer
      12th International Conference on Retinal Proteins, Awaji
    • Year and Date
      2006-06-08
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 「霊長類進化の科学」感覚受容体の退化と進化(分担執筆)2007

    • Author(s)
      今井啓雄
    • Total Pages
      11
    • Publisher
      京都大学出版会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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