| Project/Area Number |
18580073
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Nagoya University |
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Principal Investigator |
KOBAYASHI Tetsuo Nagoya University, Grad. Sch. Bioagticultural Sch, Professor (20170334)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,870,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Aspergills / Amylase / transcriptional regulation / transcription factor / AmyR / nuclear localization / 糸状菌 / Aspergillus nidulans / 核移行 / リン酸化 / 複合体 |
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| Research Abstract |
AmyR is a Zn (II)_2Cys_6 transcriptional activator that regulates expression of the amylolytic genes in Aspergillus species. Subcellular localization studies of GFP fused AmyR revealed that the fusion protein preferentially localized to the nucleus in response to isomaltose, the physiological inducer of the amylolytic genes. The C-terminal domains of AmyR, designated as MH3 (residues 419-496) and MH4 (residues 516-542), were essential for sensing the inducing stimulus and regulating the subcellular localization. The MH2 domain (residues 234-375) located in the middle of AmyR was required for transcriptional activation of the target genes, and the nuclear localization signals were identified within the N-terminal Zn (II) _2Cys_6 DNA binding motif. Mutational analysis of the MH3 domain suggested that complex liter domain interactions regulate nuclear localization and activity of AmyR
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