Elucidation of significance of serine proteases found in the intestines on cell death and the mechanism of the proteases-mediated cell death regulated by food components

• Project/Area Number: 18580118
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Kyoto University
• Principal Investigator: TSUZUKI Satoshi Kyoto University, 農学研究科, 助教 (50283651)
• Co-Investigator(Kenkyū-buntansha): INOUE Kazuo 京都大学, 大学院・農学研究科, 准教授 (80213148)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥630,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: セリンプロテアーゼ / アノイキス / アポトーシス / 腸管細胞 / 細胞代謝

Research Abstract

腸管の上皮層にはmembrane-type serine protease 1(以下MT-SP1)やgranzymeA(以下GrA)と呼ばれるセリンプロテアーゼが存在している。本研究ではGrAが古くなった腸管上皮細胞の除去に関与する可能性を示した。またMT-SP1がIEC-6細胞の細胞死を誘導するかを明らかにする目的で、本酵素のリコンビナント体の作製に着手し、その大量調製に成功した。

Research Products

• [Journal Article] The activity of a type II transmembrane serine protease, matriptase, is dependent solely on the catalytic domain (2009)
  • Author(s): Kojima K, Tsuzuki S, Fushiki T, Inouye K
  • Journal Title: Biosci. Biotechnol. Biochem 73 Pages: 454-456
  • NAID: 10027537869
  • Peer Reviewed
• [Journal Article] Roles of the stem domain of matriptase in the interaction with its physiological inhibitor, hepatocyte growth factor activator inhibitor type I (2009)
  • Author(s): Kojima K, Tsuzuki S, Fushiki T, Inouye K
  • Journal Title: J. Biochem.
  • NAID: 10025187084
  • Peer Reviewed
• [Journal Article] Roles of functional and structural domains of hepatocyte growth factor activator inhibitor type I in the inhibition of matriptase (2008)
  • Author(s): Kojima K, Tsuzuki S, Fushiki T, Inouye K
  • Journal Title: J. Biol. Chem 283 Pages: 2478-2487
  • Peer Reviewed
• [Journal Article] A lymphocyte serine protease granzyme A causes detachment of a small-intestinal epithelial cell line(IEC-6) (2008)
  • Author(s): Hirayasu H, Yoshikawa Y, Tsuzuki S, Fushiki T
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 2294-2302
  • NAID: 10027531759
  • Peer Reviewed
• [Journal Article] Granzyme A causes detachment of alveolar epithelial A549 cells accompanied by promotion of interleukin-8 release (2008)
  • Author(s): Yoshikawa Y, Hirayasu H, Tsuzuki S, Fushiki T
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 2481-2484
  • NAID: 10027532433
  • Peer Reviewed
• [Journal Article] Development of an in vitro system for screening the ligands of a membrane glycoprotein CD36 (2008)
  • Author(s): Inagaki H, Tsuzuki S, Iino T, Inoue K, Fushiki T
  • Journal Title: Cytotechnology 57 Pages: 145-150
  • Peer Reviewed
• [Journal Article] Carrageenan inhibits granzyme A-induced detachment of and interleukin-8 release from alveolar epithelial A549 cells (2008)
  • Author(s): Yoshikawa Y, Hirayasu H, Tsuzuki S, Fushiki T
  • Journal Title: Cytotechnology 58 Pages: 63-67
  • Peer Reviewed
• [Journal Article] A lymphocyte serine protease granzyme A causes detachment of a small-intestinal epithelial cell line (IEC-6) (2008)
  • Author(s): Hirayasu H, Yoshikawa, Y, Tsuzuki S, Fushiki T
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 2294-2302
  • NAID: 10027531759
  • Peer Reviewed
• [Journal Article] Roles of functional and structural domains of hepatocyte growth factor activator inhibitor type I in the inhibition of matriptase (2008)
  • Author(s): Kojima, K
  • Journal Title: J. Biol. Chem. 283 Pages: 2478-2487
  • Peer Reviewed
• [Journal Article] A role of a lymphocyte tryptase, granzyme A, in experimental ulcerative colitis (2007)
  • Author(s): Hirayasu H, Yoshikawa Y, Tsuzuki S, Fushiki T
  • Journal Title: Biosci. Biotechnol. Biochem 71 Pages: 234-237
  • NAID: 10027510632
  • Peer Reviewed
• [Journal Article] A role of a lymphocyte tryptase, granzyme A, in experimental ulcerative colitis. (2007)
  • Author(s): Hirayasu H, Yoshikawa Y, Tsuzuki S, Fushiki T.
  • Journal Title: Biosci. Biotechnol. Biochem. 71 (1) Pages: 234-237
  • NAID: 10027510632
• [Journal Article] Roles of the stem domain of matriptase in the interaction with its physiological inhibitor, hepatocyte growth factor activator inhibitor type I
  • Author(s): Kojima K, Tsuzuki S, Fushiki T, Inouye K
  • Journal Title: J. Biochem (in press)
  • NAID: 10025187084
  • Peer Reviewed
• [Presentation] MatriptaseのLDLRAドメインのHAT-1との相互作用における意義 (2009)
  • Author(s): 安元誠, ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡(マジンメッセ)
  • Year and Date: 2009-03-29
• [Presentation] Pichia pastorisを用いた matriptase 触媒ドメインの生産 (2009)
  • Author(s): 持田誠也, ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡(マリンメッセ)
  • Year and Date: 2009-03-28
• [Presentation] MatriptaseのLDLRAドメインのHAI-1との相互作用における意義 (2008)
  • Author(s): 安元誠ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡(マリンメッセ福岡)
  • Year and Date: 2008-03-29
• [Presentation] Pichia pastorisを用いたmatriptase触媒ドメインの生産 (2008)
  • Author(s): 持田誠也ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡(マリンメッセ福岡)
  • Year and Date: 2008-03-28
• [Presentation] グランザイムAによる炎症メディエーターの放出機構の解明 (2007)
  • Author(s): 都築巧ら
  • Organizer: 日本栄養・食糧学会
  • Place of Presentation: 京都(国立京都国際会館)
  • Year and Date: 2007-05-20
• [Presentation] グランザイムAによる炎症メディエーター放出機構の解明 (2007)
  • Author(s): 都築 巧
  • Organizer: 日本栄養・食糧学会
  • Place of Presentation: 京都(国立京都国際会館)
  • Year and Date: 2007-05-20
• [Presentation] グランザイムAによる腸管上皮細胞の形態変化と剥落 (2007)
  • Author(s): 平康博章ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京(東京農業大学)
  • Year and Date: 2007-03-26
• [Presentation] Membrane-type serine protease1(MT-SP1)の阻害におけるhepatocyte growth factor activator inhibitor type-1(HAI-1)の各ドメインの役割 (2007)
  • Author(s): 兒島憲二ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京(東京農業大学)
  • Year and Date: 2007-03-25