Mechanism of inhibitory activity of lenthionine against platelet aggregation and its ex vivo effect

• Project/Area Number: 18580130
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Nihon University
• Principal Investigator: KUMAGAI Hitomi Nihon University, College of Bioresource Sciences, Department of Agricultural Chemistry, Associate Professor (20225220)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: Ienthionine / shiitake mushroom / platelet aggregation / cyclic sulfuric compound / ex vivo experiment / 膜タンパク質GPIIb / IIIa / フィブリノーゲン / アグレゴメーター / フローサイトメーター

Research Abstract

Inhibiroy activity of lenthionine against signal transduction in platelets
Inhibitory activity of lenthionine, the most abundant shiitake flavor component, atainst platelet aggregation was examined. Lenthionine inhibited platelet aggregation induced by arachidonic acid and U-46619. The inhibitory activity of authentic lenthionine was almost similar to that of lenthionine in the essential oil from shiitake, indicating that lenthionine was the most active component in the essential oil.
Then, platelet was activated by using various agonists such as A23187, PMA, PAF, and ADP, to examine the inhibitory mechanizm of lenthionine. Lenthionine inhibited the aggreation of plateles induced by every agonists tested. This result indicates that lenthionie would not inhibit signal transduction cascade in platelets but inhibit adhesion and shape change of platelets, receptors for platelet activation, or phosphorylation of proteins.
Inhibition of ex vivo platelet aggregation by lenthionine
Lenthionine was orally administered to rats and blood was withdrawn after a certain interval to examine its ex vivo effect on platelet aggregation. Oral-administrated lenthionine significantly inhibited platelet aggregation 8-20 hours after the administration. Then, dose of lenthionine was varied to examine the minimum dose to exert the inhibitory activity against platelet aggregation. As a result, more than 1 mg/kg of lenthionine significantly inhibited platelet aggregation.

Research Products

• [Journal Article] Inhibition of platelet Aggregation by Orally-Administered Lenthionine,a Key Flavor Compound in Shiitake Mushrooms(Lentinula edodes)
  • Author(s): Shoichiro Shimada, Hitomi Kumagai(corresponding author), Makoto Akao, Hidetoshi Sakurai
  • Journal Title: Journal of Clinical Biochemistry and Nutrition (in press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Inhibition of Platelet Aggregation by Orally Administered Lenthionine, a Key Flavor Compound in Shiitake Mushrooms (Lentinula edodes)
  • Author(s): Shoichiro, Shimada, Hitomi, Kumagai(corresnondinz, author), Makoto, Akao, Hidetoshi, Sakurai
  • Journal Title: Journal of Clinical Biochemistry and Nutrition (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inhibition of Platelet Aggregation by Orally-Administered Lenthionine, a Key Flavor Compound in Shiitake Mushrooms(Lentinula edodes)
  • Author(s): Shoichiro Shimada, Hitomi Kumagai(corresponding author), Makoto Akao, Hidetoshi Sakurai
  • Journal Title: Journal of Clinical Biochemistry and Nutrition (in press)
  • Peer Reviewed
• [Presentation] Inhibition of ex vivo Platelet Aggregation by Lenthionine,a Key Flavor Compound in the Shiitake Mushroom,and Its Inhibitory Mechanis (2007)
  • Author(s): Shoichiro Shimada, Hidetoshi Sakurai, Hitomi Kumagai
  • Organizer: International Conference on Food Factors for Health Promotion
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2007-11-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Inhibition of ex vivo Platelet Aggregation by Lenthionine, a Key Flavor Compound in the Shiitake Mushroom, and Its Inhibitory Mechanism (2007)
  • Author(s): Shoichiro, Shimada, Hidetoshi, Sakurai, Hitomi, Kumagai
  • Organizer: nternational Conference on Food Factors for Health Promotion
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2007-11-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] レンチオニンの血小板凝集抑制作用について (2007)
  • Author(s): 島田 正一郎, 熊谷 日登美, 桜井 英敏
  • Organizer: 日本農芸化学会関東支部2007年度大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Inhibitory activity of lenthionine against platelet aggregation (2007)
  • Author(s): Shoichiro, Shimada, Hitomi, Kumagai, Hidetoshi, Sakurai
  • Organizer: Annual Meeting of Kanto Branch of the Japan Society for Bioscience, and Agrochemistry 2007
  • Place of Presentation: Utsunomiya University
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Inhibition of ex vivo Platelet Aggregation by Lenthionine, a Key Flavor Compound in the Shiitake Mushroom, and Its Inhibitory Mechanism (2007)
  • Author(s): Shoichiro Shimada, Hidetoshi Sakurai, Hitomi Kumagai
  • Organizer: International Conference on Food Factors for Health Promotion
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2007-11-10
• [Presentation] レンチオニンの血小板活性化に及ぼす影響 (2006)
  • Author(s): 島田 正一郎, 熊谷 一樹, 佐藤 由佳, 熊谷 日登美, 桜井 英敏
  • Organizer: 日本食品科学工学会第53回大会
  • Place of Presentation: 日本大学
  • Year and Date: 2006-08-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effect of lenthionine on activation of platelets (2006)
  • Author(s): Shoichiro, Shimada, Kazuki, Kumagai, Yuka, Sato, Hitomi, Kumagai, Hidetoshi, Sakurai
  • Organizer: The 53rd Annual Meeting of the Japanese Society for Food Science and Technology
  • Place of Presentation: Nihon University
  • Year and Date: 2006-08-29
  • Description: 「研究成果報告書概要(欧文)」より