Research on Identification and Characterization of Growth Factor in Abalone

• Project/Area Number: 18580190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Kitasato University
• Principal Investigator: MORIYAMA Shunsuke Kitasato University, 海洋生命科学部, 准教授 (50222352)
• Research Collaborator: TAKAHASHI Akiyoshi 北里大学, 海洋生命科学部, 教授 (10183849) ; AMANO Masafumi 北里大学, 海洋生命科学部, 教授 (10296428) ; UCHIDA Katsuhisa 新潟大学, 自然科学系, 助教 (50360508)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 成長ホルモン / 脳下垂体ホルモン / 分子進化 / アワビ / 成長促進因子 / 受容体 / 成長促進因子受容体 / サケ成長ホルモン

Research Abstract

本研究は、成長ホルモン遺伝子が無脊椎動物に起源する仮説およびサケ成長ホルモンがエゾアワビ稚貝の成長を促進する発見に基づいて、軟骨魚類と無顎類の成長促進に関与するホルモン受容体を同定した。また、アワビの脳神経節にサケの成長ホルモン抗体に対する免疫反応陽性細胞群を検出し、その組織から成長促進因子および遺伝子を単離するとともに成長促進因子受容体を探索した

Research Products

• [Journal Article] cDNA cloning and isolation of somatolactin in Mozambique tilapia andeffects of seawater acclimation,confinement stress, and fasting on itspituitary expression (2009)
  • Author(s): Uchida K, Moriyama S, Breves JP, Fox BK, Pierce AL, Borski RJ, Hirano T, Grau EG.
  • Journal Title: Gen. Comp. Endocrinol. 161 Pages: 162-170
  • Peer Reviewed
• [Journal Article] Gene structure and functional characterrrizationof growth hormone in dogfish,Squalus acanthias (2008)
  • Author(s): Moriyama S, Oda M, Yamazaki T, Yamaguchi K, Amiya N, Takahashi A, Amano M, GotoT, Nozaki M, Meguro H, Kawauchi H.
  • Journal Title: Zool. Sci. 25 Pages: 604-613
  • Peer Reviewed
• [Journal Article] Ability of salmon growth hormone to accelerate the somatic growthof juvenile abalone Haliotis discushannai (2008)
  • Author(s): Moriyama S, Tashiro K, Furukawa S, Kawauchi H.
  • Journal Title: Fis. Sci. 74 Pages: 860-866
  • Peer Reviewed
• [Journal Article] Evaluation of posttranslational processing of proopiomelanocortinin the banded houndshark pituitary bycombined cDNA cloning and massspectrometry (2008)
  • Author(s): Takahashi A, Kobayashi Y, Moriyama S, Hyodo S.
  • Journal Title: Gen. Comp. Endocrinol. 157 Pages: 41-48
  • Peer Reviewed
• [Journal Article] RFamide peptides inhibit the expression of melanotropin and growth hormone genes in the pituitary of an Agnathan, the sea lamprey, Petromyzon marinus. (2008)
  • Author(s): Moriyama, S., 他3名
  • Journal Title: Fish. Sci. 76 Pages: 860-866
  • Peer Reviewed
• [Journal Article] Gene structure and functional characterization of growth hormone in dogfish, Squalus acanthias. (2008)
  • Author(s): Moriyama, S., 他10名
  • Journal Title: Zool. Sci. 25 Pages: 604-613
  • Peer Reviewed
• [Journal Article] RFamide peptides inhibit the expression of melanotropin and growth hormone genes in the pituitary of an Agnathan,the sea lamprey, Petromyzon marinus. (2008)
  • Author(s): Moriyama, S., 他7名
  • Journal Title: Endocrinology 148 Pages: 3740-3749
  • Peer Reviewed
• [Journal Article] RFamide peptides inhibit the expression of melanotropin andgrowth hormone genes in the pituitary ofan Agnathan, the sea lamprey, Petromyzon marinus (2007)
  • Author(s): Moriyama S, Kasahara M, Amiya N, Takahashi A, Amano M, Sower SA, YamamoriK, Kawauchi H.
  • Journal Title: Endocrinology 148 Pages: 3740-3749
  • Peer Reviewed
• [Journal Article] Occurrence of two functionally distinct proopiomelanocortin genes inall modern lampreys (2006)
  • Author(s): Takahashi A, Nakata O, Moriyama S, Nozaki M, Joss MPJ, Sower SA, KawauchiK.
  • Journal Title: Gen. Comp. Endocrinol. 148 Pages: 72-78
  • Peer Reviewed
• [Journal Article] Genomic structure of the sea lamprey growth hormone-encoding gene (2006)
  • Author(s): Moriyama S, Oda M, Takahashi A, Sower SA, Kawauchi H.
  • Journal Title: Gen. Comp. Endocrinol. 148 Pages: 33-40
  • Peer Reviewed
• [Journal Article] Identification of sea lamprey GTHb-like cDNA andits evolutionary implications (2006)
  • Author(s): Sower SA, Moriyama S, Kasahara M, Takahashi A, Nozaki M, Uchida K,Dahlstrom JM, Kawauchi H.
  • Journal Title: Gen. Comp. Endocrinol. 148 Pages: 22-32
  • Peer Reviewed
• [Journal Article] Identification of sea lamprey GTHb-like cDNA and its evolutionary implications. (2006)
  • Author(s): Sower S. A, 他7名
  • Journal Title: Gen. Comp. Endocrinol. 148 Pages: 22-32
• [Journal Article] Genomic structure of the sea lamprey growth hormone-encoding gene. (2006)
  • Author(s): Moriyama S., 他4名
  • Journal Title: Gen. Comp. Endocrinol. 148 Pages: 33-40
• [Journal Article] Identification of growth hormone-releasing hormone, somatostatin and growth hormone in elasmobranch, dogfish, Triakis scyllium. (2006)
  • Author(s): Moriyama S., 他7名
  • Journal Title: Proceedings of the Japan Society for Comparative Endocrinology 21 Pages: 53-53
• [Journal Article] Growth stimulation of juvenile abalone, Haliotis discus hannai, by feeding with salmon growth hormone in sodium alginate
  • Author(s): Moriyama S, Furukawa S, Kawauchi H.
  • Journal Title: gel. Fis. Sci. (In press)
  • Peer Reviewed
• [Journal Article] Growth stimulation of juvenile abalone, Haliotis discus hannai, by feeding with salmon growth hormone in sodium alginate gel.
  • Author(s): Moriyama, S., 他2名
  • Journal Title: Fish. Sci. (in press)
  • Peer Reviewed
• [Journal Article] Ability of salmon growth hormoe to accelerate the somatic growth of juvenile abalone Haliotis discus hannai.
  • Author(s): Moriyama, S., 他3名
  • Journal Title: Fish.Sci. (in press)
  • Peer Reviewed
• [Journal Article] Gene structure and functional characterization of growth hormone in dogfish,squalus acanthias.
  • Author(s): Moriyama, S., 他10名
  • Journal Title: Zool.Sci. (in press)
  • Peer Reviewed
• [Presentation] 軟骨魚類のプロラクチン放出ペプチドホモログの単離、組織分布と生物活性 (2008)
  • Author(s): 森山俊介, 阿見弥典子, 天野勝文, 高橋明義, 兵藤晋
  • Organizer: 第33回日本比較内分泌学会大会およびシンポジウム
  • Place of Presentation: 広島
  • Year and Date: 2008-12-05
• [Presentation] 無顎類からみた腺性下垂体ホルモンの進化 (2007)
  • Author(s): 野崎眞澄, 下谷豊和, 内田勝久, 森山俊介, 高橋明義, 川内浩司, Stacia A Sower
  • Organizer: 第32回日本比較内分泌学会大会およびシンポジウム
  • Place of Presentation: 日光
  • Year and Date: 2007-10-12
• [Presentation] 魚類の成長ホルモンと成長促進機構 (2007)
  • Author(s): 森山俊介
  • Organizer: 動物学会関連集会「ホメオスタシス研究会」
  • Place of Presentation: 弘前
  • Year and Date: 2007-09-20
• [Presentation] ドチザメの成長促進に関与する視床下部・下垂体ホルモン (2006)
  • Author(s): 森山俊介
  • Organizer: 東京大学海洋研究所共同利用研究集会「軟骨魚類を探る」
  • Place of Presentation: 東京
  • Year and Date: 2006-12-01
• [Presentation] 軟骨魚類の成長促進に関与する視床下部および下垂体ホルモンの同定 (2006)
  • Author(s): 森山俊介, 熊田ひかり, 西野愛子, 前田知里, 阿見弥典子, 天野勝文, 兵藤晋, 川内浩司
  • Organizer: 第31回日本比較内分泌学会大会及びシンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2006-11-03
• [Presentation] 軟骨魚類の成長促進に関与する視床下部および下垂体ホルモンの同定 (2006)
  • Author(s): 森山俊介, 熊田ひかり, 前田知里, 阿見弥典子, 天野勝文, 兵藤晋, 川内浩司
  • Organizer: 日本下垂体研究会第21回学術集会
  • Place of Presentation: 静岡
  • Year and Date: 2006-08-03
• [Presentation] 魚類のプロラクチン放出ペプチドの構造と生物活性 (2006)
  • Author(s): 森山俊介, 阿見弥典子, 天野勝文, 兵藤晋,高橋明義, 野崎眞澄, Sower, S.A., 川内浩司.
  • Organizer: 第19回「遺伝子とその周辺」研究会
  • Place of Presentation: 相模原
  • Year and Date: 2006-08-02
• [Presentation] Structures and tissues distributions of growth hormone and insulin-like growth factor-I receptors in salmon (2006)
  • Author(s): Moriyama, S.
  • Organizer: VIIth International Congress on the Biology of Fish
  • Place of Presentation: Newfoundland, Canada
  • Year and Date: 2006-07-22
• [Presentation] 魚類のプロラクチン放出ペプチドの構造と生物活性 (2006)
  • Author(s): 森山俊介, 阿見弥典子, 天野勝文, 兵藤晋,高橋明義, 野崎眞澄, Sower, S.A., 川内浩司
  • Organizer: 第3回GPCR 研究会
  • Place of Presentation: 東京
  • Year and Date: 2006-05-13
• [Book] ホルモンハンドブック (2007)
  • Author(s): 森山俊介. 成長因子
  • Publisher: 南江堂
• [Book] 培風館. シリーズ21世紀の動物科学 (10) 内分泌と生命現象 (2007)
  • Author(s): 川内浩司, 高橋明義, 森山俊介
  • Publisher: 下垂体ホルモンの多様性
• [Book] Fish Physiology : Fish Neuroendocrinology(Vol. 28, In press)
  • Author(s): Kawauchi H, Sower SA, Moriyama S.
  • Publisher: Chapter 5 : The neuroendocrine regulation of prolactin andsomatolactin secretion. Elsevier