| Budget Amount *help |
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Research Abstract |
Listeria monocytogenes contamination of certain marine food products that are eaten raw, such as cod ovum and salmon roe, has recently been reported in Japan and has also been confirmed in our recent research. In the present study, we aimed to gain a basic understanding of the risk of L. monocytogenes contamination of marine food products by investigating its distribution and detection in marine food products that are eaten raw and by investigating the properties of L. monocytogenes isolates from food products, as well as its proliferation and viability in marine food products.
In our 2006 study, We conducted a comprehensive investigation of the distribution of L. monocytogenes and isolated strains in commercially available marine food products, including cod ovum, salmon roe, and negitoro (tuna and green onion). Furthermore, for positive samples detected using the miniVIDAS method (an official AOAC method based on immunology methods) we performed a smear test for the detection of L. monocytogenes using differential media followed by identification of typical colonies(confirmed by API and PCR. In addition, we determined the serotypes and basic genetic properties of isolates.
In our 2007 research, we continued our detailed investigation of the virulence genes of strains. Along with developing a method for typing and identifying strains, such as the development of a simple genetic typing method for L. monocytogenes based on the investigation of virulence gene inIA, we obtained basic data on the proliferation of L. monocytogenes in marine food products and under conditions for food production, such as a proliferation test for negitoro at a storage temperature of 5°C, and .evaluation of viability through evaluation of biofilm-forming ability in the food processing factory.
Based on our previous results, we were gain basic knowledge on the risk of L. monocytogenes contamination in marine food products.
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