Protein tyrosine phosphorylation in boar sperm capacitation induced by a novel relaxin-like protein
| Project/Area Number |
18580280
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Shizuoka University |
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Principal Investigator |
KOHSAKA Tetsuya Shizuoka University, Faculty of Agriculture, Professor (10186611)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Relaxin / Testis / Sperm / Capacitation / Phosphorvlation / Boar |
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| Research Abstract |
This research project was planed to elucidate protein tyrosine phosphorylation during boar sperm capacitation and acrosome reaction mediated by relaxin-like protein (seminal relaxin). The outcome of this study as follows.
The present study demonstrated that seminal relaxin actually induced boar sperm capacitation and acrosome reaction with high sperm viability. In relaxin-treated boar sperm, the activity of protein tyrosine kinase was increased with progress of the incubation time, which reached a plateau (10U/10^8 cells) at 4 h of incubation. Furthermore, Western blot analysis revealed a specific band of phosphorylated protein corresponding to the molecular size of 30 kDa in the capacitated/acrosome reacted boar sperm induced by relaxin, suggesting that relaxin induces tyrosine phosphorylation in boar sperm. Also proteome analysis characterized it as interesting and unusual. When polyclonal antibody was generated to the recombinant phosphorylated protein-MBP fusion protein expressed in E. coli, immunohistochemical analysis provided clear-cut evidence of the localization of phosphorylated protein in the sperm head and midpiece.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Effect of relaxin and IGF-I on capacitation, acrosome reaction, cholesterol efflux and utilization of labeled and unlabeled glucose in porcine spermatozoa2008
Author(s)
Miah, AG., Salma, U., Takagi, Y., Kohsaka, T., Hamano, K., Tsuji, H
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Journal Title
Reproductive Medicine and Biology 7
Pages: 29-36
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Expression and cellular pattern of relaxin mRNA in porcine corpora lutea during pregnancy2007
Author(s)
Kohsaka, T., Singh, UP., Yogo, K., Sasada, H., Taya, K., Hashizume, K
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Journal Title
Cell and Tissue Research 330
Pages: 303-312
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Effect of relaxin on the motility, acrosome reaction and utilization of glucose of fresh and frozen-thawed bovine spermatozoa2007
Author(s)
Miah, AG., Salma, U., Tareq, K., Kohsaka, T., Tsuji, H
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Journal Title
Animal Science Journal 78
Pages: 495-502
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effect of relaxin on in vitro fertilization of porcine oocytes2006
Author(s)
Young-Joon, H., Miah, AG., Yoshida, M., Sasada, H., Hamano, K., Kohsaka, T
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Journal Title
Journal of Reproduction and Development 52
Pages: 657-662
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of relaxin and its receptor LGR7 in the boar testis2006
Author(s)
Maroyama, K., Kato, S., Aoshima, T., Suchin, Itoh, H., Yogo, K., Kawarasaki, T., Yamada, K., Kitayama, Y., Kohsaka, T
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Journal Title
Proceedings of 12th AAAP Animal Science Congress XII
Pages: 1-3
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effect of relaxin on motility, acrosome reaction and viability of cryopreserved boar spermatozoa2006
Author(s)
Miah, AG., Sharoare, MD., Tareq, KM., Salma, U., Hamano, K., Kohsaka, T
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Journal Title
Reproductive Medicine and Biology 5
Pages: 215-220
Description
「研究成果報告書概要(欧文)」より
Related Report
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