Identification of transcription factors of contractile element of bronchial smooth muscle and its change in activity at airway hyperresponsiveness

• Project/Area Number: 18590246
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Hoshi University
• Principal Investigator: MISAWA Miwa Hoshi University, Sch. of Pharm., Dept. of Pharmacol, Professor (20061294)
• Co-Investigator(Kenkyū-buntansha): CHIBA Yoshihiko Hoshi University, Sch. of Pharm., Dept. of Pharmacol, Assistant professor (00287848) ; SAKAI Hiroyasu Hoshi University, Sch. of Pharm., Dept. of Pharmacol, Research Associate (00328923)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,830,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: bronchial asthma / airway hyperesponsiveness / Ca^<2+> sensitization / RhoA / transcription factor / statins / geranylgeranylation / 気道過敏性 / lovastatin / glucocorticoid / CPI-17 / Il-13 / STAT6

Research Abstract

The mechanisms of pathogenesis of airway hyperresponsiveness(AHR) in allergic bronchial asthma were investigated pharmacologically and molecular biologically. The following results were obtained.
1. The contractile responsiveness to endothelin-1(ET-1) was increased in the bronchi isolated from rats subjected with repeated antigen challenges. In the augmented ET-1-induced contractility an increase in Ca^<2+> sensitization phenomenon was involved ; phosphorylations(activations) of CTI-17 and myosin light chain kinase were enhanced. 2. The expressions and activations of matrix metalloproteinase(MMP)-12 mRNA and protein of bronchial muscle were increased by repeated antigen challenges, suggesting that MMP-12 is responsible for ABR. 3. The probable transcription factors in bronchial smooth muscle which are activated by antigen exposure were examined covering it using nuclear extraction of the muscle by the method of protein/DNA array method. Among the transcription factors activated by exposuring antigen, it was found that USF-1, Sp1, NF-El, STAT5 and STAT6 are the transcription factors that have potency to bind to the elements of RhoA promoter area 4. Interleukin-13 was demonstrated to induce AHR through the transcription factor STAT6. 5. Lovastatin, one of the statins, when administered in vivo, inhibited the AHR in rats generated by repeated antigen challenges ex they It was shown that at that time, lovastatin also inhibited the translocation of RhoA to cell membrane. These findings suggest that statins may be effective for improvement of AHR in patients with bronchial asthma. 6. It was also suggested that glucocorticoids exert effectiveness for bronchial asthma by inhibiting the RhoA upregulation seen in the bronchial smooth muscle at antigen-exposure.

Research Products

• [Journal Article] Lovastatin inhibits bronchial hyperresponsiveness by reducing RhoA signaling in rat allergic asthma (2008)
  • Author(s): Chiba, Y., Arima, J., Sakai, H. and Misawa, M.
  • Journal Title: Am.J.Physiol.Lung Cell.Mol.Physiol 294
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Probable involvement of epsilon-isoform of protein kinase C in rat bronchial smooth muscle contraction induced by acetylcholne (2008)
  • Author(s): Sakai, H., Yamamoto, M., Chiba, Y. and Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 44 Pages: 9-16
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Glucocorticoids ameliorate antigen-induced bronchial smooth muscle hyperresponsiveness by inhibiting upregulation or RhoA in rats (2008)
  • Author(s): Chiba, Y., Goto.K., Hiraha-ra, M., Sakai, H. and Misawa, M.
  • Journal Title: J.Pharmacol.Sci. 43 Pages: 615-625
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Glucocorticoids inhibited airway hyperresponsiveness through downregulation of CPI-17 in bronchial smooth muscle (2008)
  • Author(s): Goto, K., Chiba, Y., Sakai, H. and Misawa, M.
  • Journal Title: Eur.J.Pharmacol (In print)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] RhoA-kinase inhibitors augment the inhibitory effect of propofol on rat bronchial smooth muscle contaction (2008)
  • Author(s): Hanazaki, M., Yokoyama, M., Morita, K., Kohjitani, A., Sakai, H., Chiba, Y. and Misawa, M.
  • Journal Title: Anesthesia & Analgesia 106 Pages: 1765-1771
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Lovastatin inhibits bronchial hyperresponsiveness by reducing RhoA signaling in rat allergic asthma. (2008)
  • Author(s): Chiba, Y., Arima, J., Sakai, H., Misawa, M.
  • Journal Title: Am, J. Physiol. Lung Cell. Mol. Physiol. 294
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Probable involvement of epsilon-isoform of protein kinase C in rat bronchial smooth muscle contraction induced by acetylcholne. (2008)
  • Author(s): Sakai, H., Yamamoto, M., Chiba, Y., Misawa, M.
  • Journal Title: J. Smooth Muscle Res. 44 Pages: 9-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Glucocorticoids ameliorate antigen-induced bronchial smooth muscle hyper-responsiveness by inhibiting upregulation of RhoA in rats. (2008)
  • Author(s): Chiba, Y., Goto. K., Hiraha-ra, M., Sakai, H., Misawa, M.
  • Journal Title: J. Pharmacol. Sci. 43 Pages: 615-625
  • NAID: 10024320036
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Glucocorticoids inhibited airway hyperresponsiveness through downregulation of CPI-17 in bronchial smooth muscle. (2008)
  • Author(s): Goto, K., Chiba, Y., Sakai, H., Misawa, M.
  • Journal Title: Eur. J. Pharmacol (In print)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] RhoA-kinase inhibitors augment the inhibitoryeffect of propofol on rat bronchial smooth muscle contaction. (2008)
  • Author(s): Hanazaki, M., Yokoyama, M., Morita, K., Kohjitani, A., Sakai, H., Chiba, Y., Misawa, M.
  • Journal Title: Anesthesia & Analgesia 106 Pages: 1765-1771
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bronchial Asthma and Bronchial Smooth Muscle Hyperresponsiveness. (2008)
  • Author(s): Chiba, Y., Misawa, M.
  • Journal Title: Medical Bio(Ohmsa) 5(2) Pages: 44-47
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Lovastatin inhibits bronchial hyperresponsiveness by reducingRhoA signaling in rat allergic asthma. (2008)
  • Author(s): Chiba, Y., Arima, J., Sakai, H.and Misawa, M.
  • Journal Title: Am.J.Physiol.Lung Cell.Mol.Physiol 294
  • Peer Reviewed
• [Journal Article] Probable involvement of epsilon-isoform of protein kinase C inrat bronchial smooth musde contraction induced by acetylchone (2008)
  • Author(s): Sakai, H., Yamamoto, M., Chiba, Y.and Misawa, M.
  • Journal Title: J.Smooth Musde Res 44 Pages: 9-16
  • Peer Reviewed
• [Journal Article] Glucoconicoids ameliorate anbigen-induced bronchial smooth musde hyperresponsiveness by inhibiting upregulation or RhoA in rats (2008)
  • Author(s): Chiba, Y., Goto. K., Hhrahara, M., Sakai, H.and Misawa, M.
  • Journal Title: J.Pharmacol.Sci 43 Pages: 615-625
  • Peer Reviewed
• [Journal Article] Glucocorticoids inhibited airway hyperresponsiveness throughdownregUlation of CPI-17 in bronchial smooth muscle (2008)
  • Author(s): Goto, K., Chiba, Y., Sakai, H.and Misawa, M.
  • Journal Title: Eur.J.Pharmacol (In print)
  • Peer Reviewed
• [Journal Article] RhoA-kinase(ROcK)inhibitors augment the inhibitory effect of propofol on rat bronchial smooth muscle contaction (2008)
  • Author(s): Hanazaki, M., Yokoyama, M., Morita, K., Kohjitani, A., Sakai, H., Chiba, Y and Misawa, M.
  • Journal Title: Anesthesia & Analgesia (In print)
  • Peer Reviewed
• [Journal Article] Involvements of cysteinyl leukotrienes and nitric oxide in antigen-induced venodilatation of nasal mucosa in sensitized rats in vivo (2007)
  • Author(s): Chiba, Y., Oshita, M., Sakai, H. and Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 43 Pages: 139-144
  • NAID: 110006404994
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Increase in the expression of matrix metalloproteinase-12 in the airways of rats with allergic bronchial asthma (2007)
  • Author(s): Chiba, Y., Yu, Y., Sakai, H., and Misawa, M.
  • Journal Title: Biol.Pharm.Bull. 30 Pages: 318-323
  • NAID: 110006162851
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Involvements of cystei-nyl leukotrienes and nitric oxide in antigen-induced venodilatation of nasal mucosa in sensitized ratsin vivo. (2007)
  • Author(s): Chiba, Y., Oshita, M., Sakai, H., Misawa, M.
  • Journal Title: J. Smooth Muscle Res. 43 Pages: 139-144
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Increase in the expression of matrix metalloproteinase-12 in the airways of rats with allergic bronchial asthma. (2007)
  • Author(s): Chiba, Y., Yu, Y., Sakai, H., Misawa, M.
  • Journal Title: Biol. Pharm. Bull. 30 Pages: 318-323
  • NAID: 110006162851
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Involvements of cysteinyl leukotrienes and nitric oxide in antigen-induced venodilatation of nasal mucosa in sensitized rats in vivo (2007)
  • Author(s): Chiba, Y., Oshita, M., Sakai, H.and Misawa, M.
  • Journal Title: J.Smooth Muscle Res 43 Pages: 139-144
  • NAID: 110006404994
  • Peer Reviewed
• [Journal Article] Increase in the expression of matrix metalloproteinase-12 in the airways of ratswith allergic bronchial asthma (2007)
  • Author(s): Chiba, Y., Yu, Y., Sakai, H.and Misawa, M.
  • Journal Title: Biol.Pharm.Bull 30 Pages: 318-323
  • Peer Reviewed
• [Journal Article] Increase in the expression of matrix metalloproteinase-12 in the airways of rats with allergic bronchial asthma (2007)
  • Author(s): Chiba, Y., Yu, Y., Sakai, H., Misawa, M.
  • Journal Title: Biol. Pharm. Bull. 30 Pages: 318-323
  • NAID: 110006162851
• [Journal Article] Increased expression of inducible nitric oxide synthase in nasal mucosae of guinea pigs with induced allergic rhinitis (2006)
  • Author(s): Chiba, Y., Matsuo, K., Sakai, H., Misawa, M.
  • Journal Title: Am. J. Rhinol. 20 Pages: 336-341
• [Journal Article] Comparison of norepinephrine responsiveness of mucosal veins in vivo with that of isolated mucosal tissue in vitro in guinea pig nasal mucosa (2006)
  • Author(s): Chiba, Y., Oshita, M., Matsuo, K., Sakai, H., Misawa, M.
  • Journal Title: Am. J. Rhinol. 20 Pages: 349-352
• [Journal Article] Uteroglobin-related protein 1 expression suppresses allergic airway inflammation in mice (2006)
  • Author(s): Chiba, Y., Kurotani, R., Kusakabe, T., Miura, T., Link, B.W., Misawa, M
  • Journal Title: Am. J. Respir. Crit. Care Med. 173 Pages: 958-964
• [Journal Article] Role of Rho kinase in endothelin-1-induced phosphorylation of CPI-17 in rat bronchial smooth muscle (2006)
  • Author(s): Sakai, H., Chiba, Y., Misawa, M.
  • Journal Title: Pulm. Pharmacol. Ther. (Epub)
• [Journal Article] Effects of 1400W, a potent selective inducible NOS inhibitor, on histamine- and leukotriene D_4-induced relaxation of isolated guinea pig nasal mucosa (2006)
  • Author(s): Chiba, Y., Matsuo, K., Sakai, H., Abe, K., Misawa, M.
  • Journal Title: Nitric Oxide 15 Pages: 142-147
• [Journal Article] Impaired norepinephrine-mediated contraction of isolated nasal mucosa in guinea pigs with allergic rhinitis (2006)
  • Author(s): Chiba, Y., Matsuo, K., Sakai, H., Misawa, M.
  • Journal Title: Am. J. Rhinol. 20 Pages: 477-479
• [Journal Article] Augmentation of endothelin-1-induced phosphoryation of CPI-17 and myosin light chain in bronchial smooth muscle from airway hyperresponsive rats (2006)
  • Author(s): Sakai, H., Chiba, Y., Misawa, M.
  • Journal Title: Biol. Pharm. Bull. 29 Pages: 1897-1899
• [Journal Article] Regional differences in vascular responsiveness of nasal mucosae isolated from naive guinea pigs (2006)
  • Author(s): Chiba, Y., Matsuo, K., Sakai, H., Abe, K., Misawa, M.
  • Journal Title: Auris Nasus Larynx (Epub)
• [Presentation] Change in the expression of matrix metalloproteinase-12 in airways of rats with allergic bronchial asthma. (2008)
  • Author(s): Todoroki, M., Chiba, Y., Sakai, H., Misawa, M.
  • Organizer: In The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] RACK1as a binding partner for CPI-17 in bronchial smooth muscle. (2008)
  • Author(s): Tanabe, M., Chiba, Y., Kimura, S., Misawa, M.
  • Organizer: In The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Involvement of actin polymerization in antigen-induced airway hyperresponsiveness in rats. (2008)
  • Author(s): Kozutsumi, Y., Chiba, Y., Sakai, H., Misawa, M.
  • Organizer: In The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effects of cigarette smoke exposure on airway responsiveness in C57BL/6J mice. (2008)
  • Author(s): Matsuuchi, N., Ushikubo, H., Fujita, A., Sakai, H., Chiba, Y., Kamei, J., Misawa, M.
  • Organizer: In The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Development of cigarette smoke-induced bronchial muscle hyperresponsiveness in rats depends on tar and nicotine contents. (2008)
  • Author(s): Fujita, A., Ushikubo, H., Chiba, Y., Sakai, H., Kamei, J., Misawa, M.
  • Organizer: In The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of inhibitors of RhoA geranylgeranylation on antigen-induced airway hyperresponsiveness in mice. (2008)
  • Author(s): Sato S., Chiba, Y., Sakai, H., Misawa, M.
  • Organizer: In The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The role of sphingosine-1-phosphate(S1P) in airway hyperresponsiveness observed in murine allergic bronchial asthma. (2008)
  • Author(s): Takeuchi, H., Chiba, Y., Sakai, H., Misawa, M.
  • Organizer: In The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Augmented sodium nitroprusside-venodilatation of nasal mucosa in vivo in rats with allergic rhinitis. (2008)
  • Author(s): Uto, F., Enzaka, J., Oshita, M., Sakai, H., Chiba, Y., Misawa, M.
  • Organizer: In The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of a specific matrix metalloproteinase-12 inhibitor on the pathogenesis of allergic bronchial asthma. (2008)
  • Author(s): Yu, Y., Chiba, Y., Sakai, H., Misawa, M.
  • Organizer: In The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Increased expression of a sisintegrin and metalloproteinase 8 in mice with allergic vronchial asthma. (2008)
  • Author(s): Onoda, S., Chiba, Y., Sakai, H., Hattori, Y., Maitani, Y., Kimura, S., Misawa, M.
  • Organizer: In The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 気道の薬理学的研究(第312報):培養ヒト気管支平滑筋細胞における各種SOCS分子種の発現に対するIL-13の効果 (2008)
  • Author(s): 西田 雄一、千葉 義彦、轟 倫子、三澤 美和
  • Organizer: 第118回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第313報):アレルギー性気管支喘息モデルマウスの気道過敏性および気道炎症発現に及ぼすlovastatinの効果 (2008)
  • Author(s): 佐藤 駿介、千葉 義彦、三澤 美和
  • Organizer: 第118回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第314報):培養ヒト気管支平滑筋細胞でのRhoAタンパ質発現におけるTNFα/NFkB経路およびIL-13/STAT6経路の関与 (2008)
  • Author(s): 後藤 久充子、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 第118回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道平滑筋におけるカルシウム感受性調節機構と麻酔薬 (2008)
  • Author(s): 花崎 元彦、横山 正尚、森田 潔、糀谷 淳、千葉 義彦、三澤 美和
  • Organizer: 第118回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 重症度の異なる二種類のラット褥瘡モデルの開発とその応用[シンポジウム] (2008)
  • Author(s): 牛久 保裕子、千葉 義彦、三澤 美和
  • Organizer: 第118回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第311報):培養ヒト気管支平滑筋細胞におけるTNFα/NFkB誘発RhoA upregulationに対するprednisoloneの効果 (2008)
  • Author(s): 後藤 久充子、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ラット褥瘡部皮膚組織における L-arghinine 代謝酵素の発現変化 (2008)
  • Author(s): 木村 有希、古川 晃次、牛久 保裕子、酒井 寛泰医、千葉 義彦、三澤 美和
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Change in the expression of matrix metalloproteinase-12 in airways of rats with allergic bronchiall asthma (2008)
  • Author(s): Todoroki, M., Chiba, Y., Sakai, H.and Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pharmacological studies on the respiratory tract (Rept.302):RACK1 as a binding partner for CPI-17 in bronchial smooth muscle (2008)
  • Author(s): Tanabe, M., Chiba, Y., Kimura, S. & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pharmacological studies on the respiratory tract (Rept.303):Involvement of actin polymerization in antigen-induced airway hyperresponsiveness in rats (2008)
  • Author(s): Kozutsumi, Y., Chiba, Y., Salmi, H., & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pharmacological studies on the respiratory tract (Rept.304):Effects of cigarette smoke exposure on airway responsiveness in C57BL/6Jmice (2008)
  • Author(s): Matsuuchi, N., Ushikubo, H., Fujita, A., Sakai, H., Chiba, Y., Kamei, J.& Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pharmacological studies on the respiratory tract (Rept.305):Development of cigarette smoke-induced bronchial muscle hyperresponsiveness in rats depends on tar and nicotine contents (2008)
  • Author(s): Fujita, A., Ushikubo, H., Chiba, Y., Sakai, H., Kamei, J. & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pharmacological studies on the respiratory tract (Rept.306):Effect of inhibitors of RhoA geranylgeranylation on antigen-induced airway hyperresponsiveness in mice (2008)
  • Author(s): Sato S., Chiba, Y., Sakai, H.& Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pharmacological studies on the respiratory tract (Rept.307):The role of sphingosine-1-phosphate (S1P) in airway hyperresponsiveness observed in murine allergic bronchial asthma (2008)
  • Author(s): Takeuchi, H., Chiba, Y., Sakai, H. & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pharmacological studies on the respiratory tract (Rept.308):Augmented sodium nitroprusside-induced venodilatation of nasal mucosa in vivo in rats with allergic rhinitis (2008)
  • Author(s): Uto, F., Enzaka, J., Oshita, M., Sakai, H., Chiba, Y.& Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pharmacological studies on the respiratory tract (Rept.309):Effect of a specific matrix metalloproteinase-12 inhibitor on the pathogenesis of allergic bronchial asthma (2008)
  • Author(s): Yu, Y., Chiba, Y., Sakai, H., & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pharmacological studies on the respiratory tract (Rept.310):Increased expression of a disintegrin and metalloproteinase 8 in mice with allergic bronchial asthma (2008)
  • Author(s): Onoda, S., Chiba, Y., Sakai, H., Hattori, Y., Maitani, Y., Kimura, S. & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Pharmacological studies on pressure ulcer (Rept.8):A new animal model of severe pressure ulcer using Wistar rats (2008)
  • Author(s): Kusano, R., Ushikubo, H., Sakai, H., Chiba, Y.& Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第311報):培養ヒト気管支平滑筋細胞におけるTNFα/NFκ誘発RhoA upregulationに対するprednisoloneの効果 (2008)
  • Author(s): 後藤 久充子、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] ラット褥瘡部皮膚組織におけるL-arginine代謝酵素の発現変化 (2008)
  • Author(s): 木村 有希、古川 晃次、牛久保 裕子、酒井 寛泰医、千葉 義彦、三澤 美和
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] Change in the expression of matrix metalloproteinase-12 in airways of rats with allergic bronchial asthma (2008)
  • Author(s): Todoroki, M., Chiba, Y., Sakai, H. and Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Pharmacological studies on the respiratory tract(Rept.302):RACKl as a binding partner for CPI-17 in bronchial smooth muscle (2008)
  • Author(s): Tanabe, M., Chiba, Y., Kimura, S. & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Pharmacological studies on the respiratory tract(Rept.303):Involvement of actin polymerization in antigen-induced airway hyperresponsiveness in rats. (2008)
  • Author(s): Kozutsumi, Y. Chiba, Y., Sakai, H., & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Pharmacological studies on the respiratory tract(Rept.304):Effects of cigarette smoke exposure on airway responslveness in C57BL/6J mice (2008)
  • Author(s): Matsuuchi, N., Ushikubo, H., Fujita, A., Sakai, H., Chiba, Y., Kamei, J. & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Pharmacologial studies on the respiratory tract(Rept.305):Development of cigarette smoke-induced bronchial muscle hyperresponsiveness in rats depends on tar and nicotine contents (2008)
  • Author(s): Fujita, A., Ushikubo, H., Chiba, Y., Sakai, H., Kamei, J. & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Pharmacological studies on the respiratory tract(Rept.306):Effect of inhibitors of RhoA geranylgeranylation on antigen-induced airway hyperresponsiveness in mice (2008)
  • Author(s): Sato S., Chiha, Y., Sakai, H. & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Pharmacological studies on the respiratory tract(Rept.307):The role of sphingosine-1-phosphate(SIP) in airway hyperresponsiveness observed in murine allergic bronchial asthma (2008)
  • Author(s): Takeuchi, H., Chiba, Y., Sakai, H. & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Phamacological studies on the respiratory tract(Rept.308):Augmented sodium nitroprusside-induced venodilatation of nasal mucosa in vivo in rats with allergic rhinitis (2008)
  • Author(s): Uto, F., Enzaka, J., Oshita, M., Sakai, H., Chiba, Y. & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Pharmacological studies on the respiratory tract(Rept.309):Effect of a specific matrix metalloproteinase-12inhibitor on the pathogenesis of allergic bronchial asthma (2008)
  • Author(s): Yu, Y., Chiba, Y., Sakai, H, & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Pharmacological studies on the respiratory tract(Rept.310):Increased expression of a disintegrin and metalloproteinase 8 in mice with allergic bronchial asthma (2008)
  • Author(s): Onoda, S., Chiba, Y., Sakai, H., Hattori, Y., Maitani, Y., Kimura, S. & Misawa, M
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Pharmacological studies on pressure ulcer(Rept.8):A new animal model of severe pressure ulcer using Wistar rats (2008)
  • Author(s): Kusano, R., Ushikubo, H., Sakai, H., Chiba, Y. & Misawa, M.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] TNF-α 誘発 RhoA pregulation における NF-kBの関与 (2007)
  • Author(s): 後藤 久充子、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 第1回次世代を担う若手医療薬科学シンポジウム
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第299報):アレルギー性気管支喘息モデルマウスの肺組織において抗原チャレンジにより活性化されるSTATsの同定 (2007)
  • Author(s): 轟 倫子、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 第117回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第300報):培養ヒト気管支平滑筋細胞のRhoAタンパク質発現におけるTNF-α/NF-kB系の関与 (2007)
  • Author(s): 後藤 久充子、千葉 義彦、三澤 美和
  • Organizer: 第117回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第296報):アレルギー性気管支喘息マウスの肺組織におけるmatrix metalloproteinase-12発現の変化 (2007)
  • Author(s): 余 穎妍、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 第51回日本薬学会関東支部大会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第297報):アレルギー性気管支喘息マウスの肺組織におけるADAM8発現の変化 (2007)
  • Author(s): 小野田 智、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 第51回日本薬学会関東支部大会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第298報):3系統マウスにおけるタバコ主流煙誘発気道過敏性の発症とRhoAの変動 (2007)
  • Author(s): 松内 則秀、牛久 保裕子、藤田 亜希子、酒井 寛泰、千葉 義彦、三澤 美和
  • Organizer: 第51回日本薬学会関東支部大会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第295報):培養ヒト気管支平滑筋におけるIL-4およびIL-13により活性化されるSTATsの同定 (2007)
  • Author(s): 轟 倫子、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 第9回応用薬理シンポジウム
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 褥瘡の薬理学的研究(第7報):ラット褥瘡モデルの褥瘡発症・治癒過程における肥満細胞の関与 (2007)
  • Author(s): 古川 晃次、小林安 里奈、牛久 保裕子、酒井 寛泰、千葉 義彦、三澤 美和
  • Organizer: 第9回日本褥瘡学会学術大会
  • Place of Presentation: 前橋
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第293報):Prednisoloneによる抗原誘発気道過敏性抑制機序におけるIL-13/STAT6経路の関与 (2007)
  • Author(s): 後藤 久充子、酒井 寛泰、千葉 義彦、三澤 美和
  • Organizer: 第116回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第294報):IL-4およびIL-13により培養ヒト気管支平滑筋細胞で活性化されるSTATsの検討 (2007)
  • Author(s): 轟 倫子、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 第116回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第291報):ステロイドによる気道過敏性抑制はSTAT6活性化阻害を介する (2007)
  • Author(s): 後藤 久充子、酒井 寛泰、千葉 義彦、三澤 美和
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山市
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第292報):マウスアレルギー性気管支喘息時の肺組織におけるinterleukin-13受容体(ILー13Ralpha1)mRNA発現の増加 (2007)
  • Author(s): 千葉 義彦、小野田 智、轟 倫子、中沢 修司、酒井 寛泰、服部 喜之、米谷 芳枝、三澤 美和
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山市
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 褥瘡の薬理学的研究(第6報):ラット褥瘡部組織におけるMMP-9/TIMP-1バランスの変化 (2007)
  • Author(s): 牛久保 裕子、木崎 早矢香、酒井 寛泰、千葉 義彦、三澤 美和
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山市
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] TNF-α誘発RhoA pregulationにおけるNF-κBの関与 (2007)
  • Author(s): 後藤 久充子、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 第1回次世代を担う若手医療薬科学シンポジウム
  • Place of Presentation: 東京
• [Presentation] 気道の薬理学的研究(第300報):培養ヒト気管支平滑筋細胞のRhoAタンパク質発現におけるTNF-α/NF-κB系の関与 (2007)
  • Author(s): 後藤 久充子、千葉 義彦、三澤 美和
  • Organizer: 第117回日本薬理学会関東部会
  • Place of Presentation: 東京
• [Presentation] 気道の薬理学的研究(第297報):アレルギー性気管支喘息マウスの肺組織におけるN)AM8発現の変化 (2007)
  • Author(s): 小野田 智、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 第51回日本薬学会関東支部大会
  • Place of Presentation: 東京
• [Presentation] 気道の薬理学的研究(第295報):培養ヒト気管支平滑筋におけるIL-4およびIL-13により活性化されるSTATsの同定. (2007)
  • Author(s): 轟 倫子、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 第9回応用薬理シンポジウム
  • Place of Presentation: 東京
• [Presentation] 褥瘡の薬理学的研究(第5報):新規褥瘡モデルの褥瘡部皮膚組織中MMP-9の動態とその関連物質の変化 (2006)
  • Author(s): 木崎 早矢香、牛久保 裕子、小林安 里奈、三澤 美和
  • Organizer: 第8回日本褥瘡学会学術集会
  • Place of Presentation: さいたま市
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第284報):抗原誘発気道過敏性発現に対するglucocorticoidsの効果 (2006)
  • Author(s): 後藤 久充子、酒井 寛泰、千葉 義彦、三澤 美和
  • Organizer: 第8回応用薬理シンポジウム
  • Place of Presentation: 千葉市
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第285報):Lovastain 前処置による RhoA 活性化と気道過敏性の抑制 (2006)
  • Author(s): 有馬 順子、千葉 義彦、酒井 寛泰、三澤 美和
  • Organizer: 第8回応用薬理シンポジウム
  • Place of Presentation: 千葉市
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第286報):抗原誘発鼻粘膜過敏性時の鼻粘膜における血管拡張反応とNO産生の変化 (2006)
  • Author(s): 遠坂 純、大下 未知子、酒井 寛泰、千葉 義彦、三澤 美和
  • Organizer: 第8回応用薬理シンポジウム
  • Place of Presentation: 千葉市
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第287報):タバコ主流煙吸入暴露によるラットでの気道過敏性の証明 (2006)
  • Author(s): 小松 魅侑爾、牛久保 裕子、村田 真彦、松内 則秀、亀井 淳三、酒井 寛泰、千葉 義彦、三澤 美和
  • Organizer: 第8回応用薬理シンポジウム
  • Place of Presentation: 千葉市
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第288報):タバコ主流煙吸入暴露ラットではin vivo及びin vitroで気道過敏性が発症する (2006)
  • Author(s): 小松 魅侑爾、牛久保 裕子、村田 真彦、松内 則秀、亀井 淳三、酒井 寛泰、千葉 義彦、三澤 美和
  • Organizer: 第115回日本薬理学会関東部会
  • Place of Presentation: 高崎市
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第289報):Acetylcholine誘発気管支平滑筋収縮におけるアクチン重合反応の関与 (2006)
  • Author(s): 酒井 寛泰、小堤 友理、千葉 義彦、三澤 美和
  • Organizer: 第115回日本薬理学会関東部会
  • Place of Presentation: 高崎市
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 気道の薬理学的研究(第290報):抗原誘発鼻粘膜過敏性時のNO誘発鼻粘膜静脈弛緩反応の亢進とK^+channelの関与 (2006)
  • Author(s): 遠坂 純、大下 未知子、酒井 寛泰、千葉 義彦、三澤 美和
  • Organizer: 第115回日本薬理学会関東部会
  • Place of Presentation: 高崎市
  • Description: 「研究成果報告書概要(和文)」より
• [Book] メディカルバイオ (2008)
  • Author(s): 千葉 義彦、三澤 美和
  • Publisher: 株式会社オーム社
  • Description: 「研究成果報告書概要(和文)」より