| Project/Area Number |
18590839
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KOHYAMA Tadashi The University of Tokyo, Respiratory medicine, Lecturer (00302703)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIZAWA Hajime University of Teikyo, Respiratory medicine, professor (80171578)
DESAKI Masashi The University of Tokyo, Respiratory medicine, Lecturer (30251250)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Lung fibroblast / cell migration / contractility / mast cells / remodeling / inflammatory cells |
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| Research Abstract |
There are reports which show that subepithelial fibrosis in the bronchial asthma patient is related to the excess response by fibroblasts. To elucidate aging is one of the factors of remodeling; we compare the functions between 23-27 population doubling level (PDL) cells as young and 39-45 PDL cells as old. Histamine and PGD are important mediators produced by mast cells which are one of the important effecter cells in bronchial asthma. We also studied these two mediator's effects on aging and young cells. We had checked the aging fibroblast functions by using Boyden blind well chamber methods and gel contraction assay last year. Then, in this year we tried to check another fibroblast function such as protein production. As IL-8 has an important role for inducing neutrophil and eosinophil migration to the site of inflammation, we paid attention to the difference of IL-8 release by young and old fibroblasts.
We found that the ability of IL-8 production by young cells were stronger than old cells. However, the reaction of its production to the histamine and PGD2 were similar with both cells. It was shown that old cells function has just become weak, but still reacted well. Aging weakens the fibroblast functions even remodeling process. The older patient become, the weaker disease activity would be.
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