| Project/Area Number |
18591189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KAGAMI Shoji The University of Tokushima, Institute of Health Biosciences, Graduate School, Professor (00224337)
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| Co-Investigator(Kenkyū-buntansha) |
URUSHIHARA Maki The University of Tokushima, Institute of Health Biosciences, Graduate School, Assistant Professor (50403689)
ROKUTAN Kazuhito The University of Tokushima, Institute of Health Biosciences, Graduate School, Professor (10230898)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | ILK / Integrin / Parietal epithelial cell / ROS / NAD(P)H oxidase / rescent cell / Glomerulosclerosis / Renal failure |
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| Research Abstract |
In order to clarify the role of ROS in the integrin-dependent signaling involved in progressive glomerulonephritis (GN), we investigated the localization and function of NAD(P)H oxidase -derived ROS signaling using a model of experimental GN (WKY masugi GN) and cultured parietal epithelial cells(PEC). Glomerular and crescent expression of NAD(P)H and DHE was increased in parallel with PEC proliferation and matrix accumulation during the course of GN. Inhibition of ROS production with angiotensin receptor inhibitor (ARB) in this GN model revealed that NAD(P)H oxidase-derived ROS signaling play an important role in PEC proliferation, matrix remodeling and crescent formation in progressive GN. Immunofluorescent study with cultured PEC showed that other Nox proteins except Nox2 may play a role in NAD(P)H oxidase oxidase-derived ROS signaling seen in progressive GN and crescent formation. These results indicate that NAD(P)H oxidase-derived ROS signaling play a critical role in crescent formation in progressive GN. Further study is required for development of pharmacological tool to inhibit NAD(P)H oxidase-derived ROS signaling involved in progression of GN in human.
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