Analysis of intracellular mechanisms and related factors regarding spinal protection by remote preoonditioning
| Project/Area Number |
18591697
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Gifu University |
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Principal Investigator |
IIDA Hiroki Gifu University, Graduate School of Medicine, Associate Professor (30159561)
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| Co-Investigator(Kenkyū-buntansha) |
IIDA Mami Gifu University, Graduate School of Medicine, Part time lecture (80350859)
DOHI Shuji Gifu University, Graduate School of Medicine, Professor (40155627)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,550,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Aortic clamp and unclamp / Spinal ischemia / Remote preconditioning / Angiotensin II / Rho-kinase / Free radical / Spinal cord tissue oxygen pressure / Limb ischemia / プレコンディショニング / 脳血管 |
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| Research Abstract |
Pentobarbital anesthetized rabbits were prepared for measurement of cerebral pial vessel diameter in a cranial window preparation or spinal cord partial tissue oxygen pressure (PtO2). We investigated the experiments according to following protocols.
1) We studied the effect of aortic clamping and declamping as a remote preconditioning intervention on cerebral pial vessel diameter. Although infrarenal aortic cross-clamping did not affect pial vessel diameter, release of a 20-min aortic cross-clamp caused pial arterioles to dilate and then constrict. A significant constriction persisted for at least 60 min. To clarify the mechanism of remote preconditioning regarding humoral factor, we examined whether angiotensin II typel receptor blocker, Rho-kinase inhibitor, or free radical scavenger, would affect the respose observed after aortic cross-clamping and release on cerebral vessels. All three drugs significantly attenuated the constriction of pial arterioles. Thus angiotensin II, Rho-kinase, and free radical could be possible to be candidates for humoral factors related to remote conditioning on spinal protection.
2) We measured directly the tissue oxygen partial pressure of the spinal cord to assess the efficacy of remote preconditioning in preventing apinal cord ischemic injury in an experimental model. PtO2 were significantly lower in the preconditioning intervention group at 20 min after aortic cross-clamping and 0, 2 min after aortic unclamping. Remote preconditioning performed in fore and hind legs protected the decrease of PtO2 compared with control group. Thus, the effect on PtO2 by such intervention may relate to the mechanism of spinal protection induced by remote preconditioning by transient upper and lower limbs ischemia.
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Report
(3 results)
Research Products
(61 results)
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[Journal Article] Effects of transient cerebral ischemia on vasopressin-induced vasoconstriction in rabbit cerebral vessels2008
Author(s)
Kumazawa, M., Iida, H., Uchida, M., Iida, M., Takenaka, M., Fukuoka, N., Michino, T., Dohi S
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Journal Title
Anesth Analg 106
Pages: 910-915
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The comparative effects of intravenous nicardipine and prostaglandin E1 on the cerebral pial arteriolar constriction seen after unclamping of an aortic cross-clamp in rabbits2007
Author(s)
Kumazawa, M., Iids, H., Uchida, M., Iida, M., Takenaka, M., Dohi, S
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Journal Title
Anesth Analg 104
Pages: 659-65
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Surgical treatment of scoliosis associated with central core disease : minimizing the effects of malignant hyperthermia with provocation tests2007
Author(s)
Miyamoto, K., Shimizu, K., Matsumoto, S., Sumida, H., Iida, H., Hosoe, H
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Journal Title
J.Pediatr Orthop B 16
Pages: 239-242
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Effects of ifenprodil on valtage-gated tetrodtoxin-resistant Na+ Channels in rat sensory neurons2007
Author(s)
Tanahashi, S., Iida, H., Oda, A., osawa, Y., Uchida, M., Dohi, S
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Journal Title
Eur J Anaesth 24
Pages: 782-788
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Effects of alpha2-adrenoceptor agonists on tetrodotoxin-resistant Na channels in rat dorsal root ganglion neurons2007
Author(s)
Oda, A., Iida, H., Tanahashi, S., Osawa, Y., Yamaguchi, S., Dohi, S
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Journal Title
Eur J Anaesthesiol 24
Pages: 934-941
Description
「研究成果報告書概要(欧文)」より
Related Report
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