Development of novel bioabsorbable synthetic grafts for ureteral regeneration
| Project/Area Number |
18591750
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Hyogo College of Medicine (2007)
Kyoto University (2006) |
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Principal Investigator |
YAMAMOTO Shingo (2007) Hyogo College of Medicine, Faculty of Medicine, Associate Professor (80322741)
伊藤 哲之 (2006) 京都大学, 医学研究科, 講師 (70343225)
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| Co-Investigator(Kenkyū-buntansha) |
TABATA Yasuhiko Kyoto University, Institute for Frontier Medical Sciences, Professor (50211371)
OGAWA Osamu Kyoto University, Graduate School of Medicine, Faculty of Medicine, Professor (90260611)
SODA Takeshi Kyoto University, Graduate School of Medicine, Faculty of Medicine, Instructor (50422928)
山本 新吾 兵庫医科大学, 医学部, 助教授 (80322741)
高橋 毅 京都大学, 医学研究科, 助手 (30362487)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Ureteral regeneration / PLA-CL mesh / Collagen coating / bFGF sustained-release geratin sheet |
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| Research Abstract |
We have previously reported that the deficient urethras had been successfully regenerated by surgically replacing with the bioabsorbable synthetic tube grafts composed of poly-(lactide/ -caprolactone) copolymer and collagen (Kanatani, et. Al.: Tissue Engineering 13: 2933,2007). Based on these techniques and results, the tube grafts composed of the identical materials were prepared for urethral regeneration. However, unfortunately, the replacement of the rabbit ureter was technically difficult due to the fine diameters of those.
Next, we hypothesized that the urinary tract can be repaired by simply wrapping from outside with appropriate materials. At this point, we focused on the behavior of smooth muscle and extracellular matrix. To simplify our experimental design, the rat bladder wall was covered by a gelatin sheet releasing bFGF. As a result, bFGF promoted the bladder smooth muscle proliferation and matrix remodeling, which reinforced the overall bladder contractility (Imamura et.al.:Imamura, et. Al.: American Journal of Physiology. Renal Physiology 293:F1007,2007).
Finally, the rat ureter was wrapped with a bFGF sustained-release geratin sheet. Two weeks later urethral wall hypertrophy associated with a capillary angiogenesis was observed. There will be a need to elucidate whether this phenomenon contributes to functional regeneration of the ureter.
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Report
(3 results)
Research Products
(32 results)
