| Project/Area Number |
18591767
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Nagoya City University |
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Principal Investigator |
KAWAI Noriyasu Nagoya City University, Graduate School of Medical Sciences, Researcher (20254279)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Akira Kyusyu University, Graduate School of Engineering, Associate Professor (60345915)
TOZAWA Keiichi Nagoya City University, Graduate School of Medical Sciences, Associate Professor (40264733)
KOHRI Kenjiro Nagoya City University, Graduate School of Medical Sciences, Professor (30122047)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,950,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | prostate cancer / bone metastasis / hyperthermia / osteoclast / cell proliferation / bone destruction / リボソーム / 磁場誘導組織内加温法 |
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| Research Abstract |
BACKGROUND: We have developed magnetite nanoparticles conjugated with cationic liposomes (MCLs) to induce intracellular hyperthermia with exposure to an alternating magnetic field (AMF). We have previously demonstrated the hyperthermic effect of MCLs against certain types of malignant tumor cells in vivo. Here, we examine the effects of MCL+AMF heat therapy on prostate cancer tissue in a bone microenvironment and on bone destruction in a rat model.
MATERIALS AND METHODS: Rat prostate cancer nodules were transplanted onto the calvaria of 6-week-old F344 male rats. MCLs were injected into the tumor which reached 7 mm in diameter, and then the animals were exposed to repeated AMF irradiation. The distribution of MCL, tumor necrosis, cell proliferation and bone destruction in the bone microenvironment were evaluated.
RESULTS: MCL+AMF heat therapy suppressed tumor growth on the calvaria, and histologically, the induction of a necrotic mass was observed around magnetic particles in the tumor. The bone destruction index, which indicates the degree of osteolysis associated with prostate tumor growth in the bone microenvironment, was 34.8% in the MCL group and 67.2% in the control group with significant difference. However, almost half of rats were dead in this experiment.
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