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A study on mechanisms of tissue repair and regeneration of the dentin-pulp complex

Research Project

Project/Area Number 18592085
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionNiigata University

Principal Investigator

YOSHIBA Kunihiko  Niigata University, 医歯学系, 准教授 (30220718)

Co-Investigator(Kenkyū-buntansha) YOSHIBA Nagako  新潟大学, 医歯学系, 助教 (10323974)
HOSOYA Akihiro  松本歯科大学, 歯学部, 助教 (70350824)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywords象牙質・歯髄複合体 / 修復・再生 / 歯髄組織幹細胞 / 象牙芽細胞 / 直接覆髄 / レーザー / 免疫組織化学 / BrdU / nestin / osteopontin / 組織幹細胞 / 再植 / 移植 / 非コラーゲン性タンパク / Green fluorescent protein / 再生 / TIMPs / α-SMA
Research Abstract

象牙質・歯髄複合体の修復・再生過程における歯髄組織幹細胞/前駆細胞の動態と象牙芽細胞への分化機構を解明する目的で, 直接覆髄処置, レーザー照射, あるいは歯の再植, 歯髄組織の移植を行い, その後の硬組織形成過程について免疫組織化学的に観察した。その結果, 歯髄組織中に存在する歯髄固有細胞から分化した象牙芽細胞様細胞あるいは骨芽細胞様細胞によって硬組織形成が行われることが明らかにされた。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (39 results)

All 2008 2007 2006

All Journal Article (16 results) (of which Peer Reviewed: 12 results) Presentation (23 results)

  • [Journal Article] Immunohistochemical analysis of nestin, osteopontin, and proliferating cells in the reparative process of exposed dental pulp capped with mineral trioxide aggregate2008

    • Author(s)
      Kuratate M, Yoshiba K, Shigetani Y, Yoshiba N, Ohshima H, Okiji T.
    • Journal Title

      Journal of Endodontics 34

      Pages: 970-974

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Alveolar bone regeneration of subcutaneously transplanted rat molar2008

    • Author(s)
      Hosoya A, Ninomiya T, Hiraga T, Zhao C, Yoshiba K, Yoshiba N, Takahashi M, Okabe T, Wakitani S, Yamada H, Kasahara E, Ozawa H, Nakamura H.
    • Journal Title

      Bone 42

      Pages: 350-357

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Alveolar bone regeneration of subcutaneously transplanted rat molar.2008

    • Author(s)
      Akihiro Hosoya
    • Journal Title

      Bone 42

      Pages: 350-357

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical analysis of nestin, osteopontin, and prolif erating cells in the reparative process of exposed dental pulp capped with mineral trioxide aggregate.2008

    • Author(s)
      Momoko Kuratate
    • Journal Title

      Journal of Endodontics 34

      Pages: 970-974

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of TIMP-2 with extracellular matrix exposed to mechanical stress and its co-distribution with periostin during mouse mandible development2007

    • Author(s)
      Yoshiba N, Yoshiba K, Hosoya A, Saito M, Yokoi T, Okiji T, Amizuka N, Ozawa H.
    • Journal Title

      Cell and Tissue Research 330

      Pages: 133-145

    • Related Report
      2008 Final Research Report
  • [Journal Article] Immunohistochemical study of hard tissue formation in the rat pulp cavity after tooth replantation2007

    • Author(s)
      Zhao C, Hosoya A, Kurita H, Hu T, Hiraga T, Ninomiya T, Yoshiba K, Yoshiba N, Takahashi M, Kurashina K, Ozawa H, Nakamura H.
    • Journal Title

      Archives of Oral Biology 52

      Pages: 945-953

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hard tissue formation in subcutaneously transplanted rat dental pulp2007

    • Author(s)
      Hosoya A, Nakamura H, Ninomiya T, Hoshi K, Yoshiba K, Yoshiba N, Takahashi M, Okabe T, Sahara N, Yamada H, Kasahara E, Ozawa H.
    • Journal Title

      Journal of Dental Research 86

      Pages: 469-474

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Association of TIMP-2 with extracellular matrix exposed to mechanical stress and its co-distribution with periostin during mouse mandible development.2007

    • Author(s)
      Nagako Yoshiba
    • Journal Title

      Cell and Tissue Research 330

      Pages: 133-145

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hard tissue formation in subcutaneously transplanted rat dental pulp.2007

    • Author(s)
      Akihiro Hosoya
    • Journal Title

      Journal of Dental Research 86

      Pages: 469-474

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical study of hard tissue formation in the rat pulp cavity after tooth replantation.2007

    • Author(s)
      Chen Zhao
    • Journal Title

      Archives of Oral Biology 52

      Pages: 945-953

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical localization of alpha-Smooth muscle actin during rat molar tooth development2006

    • Author(s)
      Hosoya A, Nakamura H, Ninomiya T, Yoshiba K, Yoshiba N, Nakaya H, Wakitani S, Yamada H, Kasahara E, Ozawa H.
    • Journal Title

      Journal of Histochemistry&Cytochemistry 54

      Pages: 1371-1378

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical study of osteodentin in the unerupted rat incisor2006

    • Author(s)
      Hosoya A, Nakamura H, Akahane S, Yoshiba K, Yoshiba N, Ninomiya T, Hoshi K, Sahara N, Kasahara E, Ozawa H.
    • Journal Title

      Journal of Oral Biosciences 48

      Pages: 132-137

    • NAID

      10027102099

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential regulation of TIMP-1,-2, and-3 mRNA and protein expressions during mouse incisor development2006

    • Author(s)
      Yoshiba N, Yoshiba K, Stoetzel C, Perrin-Schmitt F, Cam Y, Ruch JV, Hosoya A, Ozawa H, Lesot H.
    • Journal Title

      Cell and Tissue Research 324

      Pages: 97-104

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential regulation of TIMP-1,-2, and -3 mRNA and protein expressions during mouse incisor development.2006

    • Author(s)
      Nagako Yoshiba
    • Journal Title

      Cell and Tissue Research 324・1

      Pages: 97-104

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Immunohistochemical study of osteodentin in the unerupted rat incisor.2006

    • Author(s)
      Akihiro Hosoya
    • Journal Title

      Journal of Oral Biosciences 48・2

      Pages: 132-137

    • NAID

      10027102099

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Immunohistochemical localization of alpha-smooth muscle actin during rat molar tooth development.2006

    • Author(s)
      Akihiro Hosoya
    • Journal Title

      Journal of Histochemistry & Cytochemistry 54・12

      Pages: 1371-1378

    • Related Report
      2006 Annual Research Report
  • [Presentation] 半導体レーザー照射後のラット臼歯歯髄初期反応に関する免疫組織学的検討2008

    • Author(s)
      笹なつき, 重谷佳見, 鈴木啓展, 吉羽邦彦, 吉羽永子, 興地隆史
    • Organizer
      日本歯科保存学会2008年度秋季学術大会(第129回)
    • Place of Presentation
      富山
    • Year and Date
      2008-11-07
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Er : YAG レーザーによる窩洞形成後のラット臼歯歯髄反応に関する免疫組織学的検討2008

    • Author(s)
      重谷佳見, 鈴木啓展, 笹なつき, 吉羽邦彦, 山中裕介, 吉羽永子, 興地隆史
    • Organizer
      日本歯科保存学会2008年度秋季学術大会(第129回)
    • Place of Presentation
      富山
    • Year and Date
      2008-11-07
    • Related Report
      2008 Final Research Report
  • [Presentation] Er : YAGレーザーによる窩洞形成後のラット臼歯歯髄反応に関する免疫組織学的検討2008

    • Author(s)
      重谷佳見
    • Organizer
      日本歯科保存学会2008年度秋季学術大会(第129回)
    • Place of Presentation
      富山
    • Year and Date
      2008-11-07
    • Related Report
      2008 Annual Research Report
  • [Presentation] Mineral Trioxide Aggregate(MTA)に対するヒト歯髄反応に関する免疫組織化学的研究2008

    • Author(s)
      吉羽邦彦, 吉羽永子, 重谷佳見, 細矢明宏, 中村浩彰, 興地隆史
    • Organizer
      第50回歯科基礎医学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2008-09-25
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Tissue Responses of Human Dental Pulps to Mineral Trioxide Aggregate2008

    • Author(s)
      Yoshiba K, Yoshiba N, Shigetani Y, Hosoya A, Okiji T.
    • Organizer
      IADR 86th General Session&Exhibition
    • Place of Presentation
      トロント(カナダ)
    • Year and Date
      2008-07-04
    • Related Report
      2008 Final Research Report
  • [Presentation] Reparative Dentinogenesis of Rat Molars Pulp-capped with Mineral Trioxide Aggregate2008

    • Author(s)
      Kuratate M, Yoshiba K, Shigetani Y, Yoshiba N, Ohshima H, Okiji K.
    • Organizer
      IADR 86th General Session&Exhibition
    • Place of Presentation
      トロント(カナダ)
    • Year and Date
      2008-07-04
    • Related Report
      2008 Final Research Report
  • [Presentation] Tissue responses of human dental pulps to mineral trioxide aggregate.2008

    • Author(s)
      Kunihiko Yoshiba
    • Organizer
      86th International Association for Dental Research
    • Place of Presentation
      トロント(カナダ)
    • Year and Date
      2008-07-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] Reparative dentinogenesis of rat molars pulp-capped with mineral trioxide aggregate.2008

    • Author(s)
      Momoko Kuratate
    • Organizer
      86th International Association for Dental Research
    • Place of Presentation
      トロント(カナダ)
    • Year and Date
      2008-07-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] 再植歯の歯髄腔内における骨様組織形成2008

    • Author(s)
      細矢明宏, Zhao Chen, 吉羽邦彦, 吉羽永子, 山田博仁, 笠原悦男, 小澤英浩, 中村浩彰
    • Organizer
      日本歯科保存学会2008年度春季学術大会(第128回)
    • Place of Presentation
      新潟
    • Year and Date
      2008-06-05
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Mineral Trioxide Aggregate(MTA)を応用した象牙質再生療法-直接覆髄後の歯髄反応について-2008

    • Author(s)
      吉羽邦彦, 吉羽永子, 鞍立桃子, 重谷佳見, 興地隆史
    • Organizer
      第21回日本歯科医学会総会
    • Place of Presentation
      横浜
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] MTAに対するヒト歯髄反応に関する免疫組織化学的研究-直接覆髄後の細胞外基質の変化と細胞分化-2007

    • Author(s)
      吉羽永子, 吉羽邦彦, 重谷佳見, 細矢明宏, 興地隆史
    • Organizer
      日本歯科保存学会2007年度秋季学術大会(第127回)
    • Place of Presentation
      岡山
    • Year and Date
      2007-11-08
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] Association of TIMP-2 with extracellular matrix exposed to mechanical stress and its co-distribution with periostin during mouse tooth development2007

    • Author(s)
      Yoshiba N, Yoshiba K, Hosoya A, Saito M, Yokoi T, Okiji T, Amizuka N, Ozawa H.
    • Organizer
      9th International Conference on Tooth Morphogenesis and Differentiation
    • Place of Presentation
      チューリッヒ(スイス)
    • Year and Date
      2007-09-06
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] MTAによるラット臼歯覆髄モデルにおける歯髄反応2007

    • Author(s)
      鞍立桃子, 吉羽邦彦, 重谷佳見, 吉羽永子, 大島勇人, 興地隆史
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      札幌
    • Year and Date
      2007-08-30
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] Mineral Trioxide Aggregate(MTA)に対するヒト歯髄の反応-直接覆髄後の硬組織形成過程の免疫組織化学的研究-2007

    • Author(s)
      吉羽邦彦, 吉羽永子, 重谷佳見, 細矢明宏, 興地隆史
    • Organizer
      日本歯科保存学会2007年春季学術大会(第126回)
    • Place of Presentation
      大宮
    • Year and Date
      2007-06-08
    • Related Report
      2008 Final Research Report
  • [Presentation] Mineral Trioxide Aggregate(MTA)に対するヒト歯髄の反応-直接覆髄後の硬組織形成過程の免疫組織化学的研究-2007

    • Author(s)
      吉羽邦彦
    • Organizer
      日本歯科保存学会2007年度春季学術大会(第126回)
    • Place of Presentation
      大宮
    • Year and Date
      2007-06-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] Mineral Trioxide Aggregate(MTA)に対するラット臼歯歯髄の反応に関する免疫組織化学的研究2007

    • Author(s)
      鞍立桃子, 吉羽邦彦, 重谷佳見, 吉羽永子, 興地隆史
    • Organizer
      第28回日本歯内療法学会学術大会
    • Place of Presentation
      広島
    • Year and Date
      2007-05-26
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] Reparative dentinogenesis of exposed dental pulp capped with mineral trioxide aggregate : An immunohistochemical study2007

    • Author(s)
      Okiji T, Kuratate M, Shigetani Y, Yoshiba N, Ohshima H, Yoshiba K.
    • Organizer
      International symposium on molecular destruction and reconstruction of the dentin/pulp complex and its surrounding tissues
    • Place of Presentation
      東京
    • Year and Date
      2007-03-05
    • Related Report
      2008 Final Research Report
  • [Presentation] Mineral Trioxide Aggregate(MTA)に対するラット臼歯歯髄の反応-硬組織形成過程の免疫組織化学的研究-2006

    • Author(s)
      鞍立桃子, 吉羽邦彦, 重谷佳見, 吉羽永子, 興地隆史
    • Organizer
      日本歯科保存学会2006年秋季学術大会(第125回)
    • Place of Presentation
      鹿児島
    • Year and Date
      2006-11-09
    • Related Report
      2008 Final Research Report
  • [Presentation] ラット臼歯皮下移植後の歯槽骨再生2006

    • Author(s)
      細矢明宏, 中村浩彰, 吉羽邦彦, 吉羽永子, 山田博仁, 笠原悦男, 小澤英浩
    • Organizer
      日本歯科保存学会2006年秋季学術大会(第125回)
    • Place of Presentation
      鹿児島市民文化ホール
    • Year and Date
      2006-11-09
    • Related Report
      2008 Final Research Report
  • [Presentation] マウス歯の発生過程におけるTIMP-2とPeriostinは類似の時間的空間的発現パターンを示す2006

    • Author(s)
      吉羽永子, 吉羽邦彦, 興地隆史, 斎藤正寛, 横井隆政, 細矢明宏, 網塚憲生, 小澤英浩
    • Organizer
      第48回歯科基礎医学会学術大会
    • Place of Presentation
      横浜
    • Year and Date
      2006-09-23
    • Related Report
      2008 Final Research Report
  • [Presentation] 歯根膜組織の歯槽骨再生能2006

    • Author(s)
      細矢明宏, 中村浩彰, 二宮禎, 平賀徹, Zhao Chen, 吉羽邦彦, 吉羽永子, 笠原悦男, 小澤英浩
    • Organizer
      第48回歯科基礎医学会学術大会
    • Place of Presentation
      横浜
    • Year and Date
      2006-09-22
    • Related Report
      2008 Final Research Report
  • [Presentation] α-平滑筋アクチンの歯周組織発生過程における局在と歯槽骨形成との関連2006

    • Author(s)
      細矢明宏, 中村浩彰, 二宮禎, 吉羽邦彦, 中谷宏幸, 脇谷滋之, 山田博仁, 笠原悦男, 小澤英浩
    • Organizer
      第24回日本骨代謝学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2006-07-08
    • Related Report
      2008 Final Research Report
  • [Presentation] ラット臼歯皮下移植実験モデルにおける歯槽骨再生に関する免疫組織化学的研究2006

    • Author(s)
      細矢明宏, 中村浩彰, 吉羽邦彦, 岡部高弘, 脇谷滋之, 小澤英浩
    • Organizer
      第9回日本組織工学会
    • Place of Presentation
      京都
    • Related Report
      2008 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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