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ブタ由来イムノバイオティクスによるエピジェネティック修飾の家畜健全育成応用戦略

Research Project

Project/Area Number 18F18081
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Animal production science
Research InstitutionTohoku University
Host Researcher 北澤 春樹  東北大学, 農学研究科, 教授 (10204885)
Foreign Research Fellow ISLAM MD. Aminul  東北大学, (連合)農学研究科(研究院), 外国人特別研究員
Project Period (FY) 2018-10-12 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2020: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2019: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2018: ¥500,000 (Direct Cost: ¥500,000)
Keywordsイムノバイオティクス / イムノジェニクス / ブタ / 家畜 / エピジェネティック / 抗菌剤代替
Outline of Annual Research Achievements

近年、乳酸菌やビフィズス菌に代表されるプロバイオティクスの中でも、特に粘膜免疫調節機能性を発揮するイムノバイオティクスが注目され、家畜健全育成の飛躍的向上を目指した抗菌剤代替としての発展的利用性が期待されている。申請者らはこれまでに、抗菌剤代替としてのイムノバイオティクスの家畜対応型選抜・評価系の確立を手掛けてきた。また、外国人特別研究員は、家畜の生体防御免疫機能性におけるジェネティックおよびエピジェネティックな解析を進めてきた。そこで本研究では、それらの研究領域を発展的に融合させ、世界共通課題である薬剤のみに頼らない家畜健全育成技術の飛躍的向上から安全な畜産食品生産に貢献することを目標に、とりわけイムノバイオティクスによる粘膜免疫機構のエピジェネティック修飾に着目した新たな研究基盤を構築し、家畜対応型イムノバイオティクスの発展的利用性について追究する。本年度は、以下の通り、イムノバオティクスの有力候補菌株について、全ゲノム配列解析を行い、イムノバイオティクスによるエピジェネチィック修飾に係わるイムノジェニクス(イムノバイオティクスが有する活性因子)を解析し、その一端が明らかとなった。
1. イムノバイオティクスのゲノム配列解析とイムノジェニクス候補の選抜:イムノバオティクスの有力候補菌株について、全ゲノム配列解析を行い、比較・機能的ゲノミクス解析により、イムノジェニクス候補が選抜できた。2. イムノジェニクス候補因子のノックアウト株の作成と機能評価:1の候補因子についてイムノバイオティクス候補菌のノックアウト株を作成し、これまでに構築した評価方法を用いて野生株との比較による効果検証を推進した。3. インビボ試験による評価:イムノバイオティクス候補菌の一部の野外株について、ブタにおけるインビボ試験を行い、免疫賦活化効果をはじめとする有益性が認められた。

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(32 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (6 results) Journal Article (12 results) (of which Int'l Joint Research: 12 results,  Peer Reviewed: 12 results,  Open Access: 12 results) Presentation (9 results) (of which Int'l Joint Research: 4 results) Book (1 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Bangladesh Agricultural University(バングラデシュ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] CERELA-CONICET(アルゼンチン)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Concepcion(チリ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] CERELA-CONICET(アルゼンチン)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Concepcion(チリ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] CERELA-CONICET(アルゼンチン)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Selection of Immunobiotic Ligilactobacillus salivarius Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies2020

    • Author(s)
      Binghui Zhou, Leonardo Albarracin, Yuhki Indo, Lorena Arce, Yuki Masumizu, Mikado Tomokiyo, Md. Aminul Islam, Valeria Garcia-Castillo, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Hidetoshi Morita, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa
    • Journal Title

      Microorganisms

      Volume: 8 Pages: 1659-1659

    • DOI

      10.3390/microorganisms8111659

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Functional and Genomic Characterization of Ligilactobacillus salivarius TUCO-L2 Isolated From Lama glama Milk: A Promising Immunobiotic Strain to Combat Infections2020

    • Author(s)
      Quilodran-Vega Sandra、Albarracin Leonardo、Mansilla Flavia、Arce Lorena、Zhou Binghui、Islam Md Aminul、Tomokiyo Mikado、Al Kassaa Imad、Suda Yoshihito、Kitazawa Haruki、Villena Julio
    • Journal Title

      Frontiers in Microbiology

      Volume: 11

    • DOI

      10.3389/fmicb.2020.608752

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Draft Genome Sequence of Ligilactobacillus salivarius FFIG58, Isolated from the Intestinal Tract of Wakame-Fed Pig2020

    • Author(s)
      Zhou Binghui、Albarracin Leonardo、Masumizu Yuki、Indo Yuhki、Islam M. Aminul、Garcia-Castillo Valeria、Ikeda-Ohtsubo Wakako、Suda Yoshihito、Aso Hisashi、Villena Julio、Kitazawa Haruki
    • Journal Title

      Microbiology Resource Announcements

      Volume: 9

    • DOI

      10.1128/mra.00839-20

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of the Immunomodulatory Ability of Lactic Acid Bacteria Isolated from Feedlot Cattle Against Mastitis Using a Bovine Mammary Epithelial Cells In Vitro Assay2020

    • Author(s)
      Fukuyama Kohtaro、Islam Md. Aminul、Takagi Michihiro、Ikeda-Ohtsubo Wakako、Kurata Shoichiro、Aso Hisashi、Vignolo Graciela、Villena Julio、Kitazawa Haruki
    • Journal Title

      Pathogens

      Volume: 9 Pages: 410-410

    • DOI

      10.3390/pathogens9050410

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lipoteichoic acid is involved in the ability of the immunobiotic strain Lactobacillus plantarum CRL1506 to modulate the intestinal antiviral innate immunity triggered by TLR3 activation.2020

    • Author(s)
      Hiroya Mizuno, Lorena Arce, Kae Tomotsune, Leonardo Albarracin, Ryutaro Funabashi, Daniela Vera, Md. Aminul Islam, Maria Guadalupe Vizoso-Pinto, Hideki Takahashi, Yasuko Sasaki, Haruki Kitazawa and Julio Villena
    • Journal Title

      Frontiers in Immunology

      Volume: 11 Pages: 571-571

    • DOI

      10.3389/fimmu.2020.00571

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection2020

    • Author(s)
      Garcia-Castillo, V., H. Kitazawa et al.
    • Journal Title

      Microorganisms

      Volume: 8:479 Pages: 1-20

    • DOI

      10.3390/microorganisms8040479

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficient Selection of New Immunobiotic Strains With Antiviral Effects in Local and Distal Mucosal Sites by Using Porcine Intestinal Epitheliocytes2020

    • Author(s)
      Leonardo, A., V. Garcia-Castillo, H. Kitazawa et al.
    • Journal Title

      Frontiers in Immunology

      Volume: 11:543 Pages: 1-16

    • DOI

      10.3389/fimmu.2020.00543

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In-Vitro Cell Culture for Efficient Assessment of Mycotoxin Exposure, Toxicity and Risk Mitigation2020

    • Author(s)
      Xu, R., N. A. Karrow H. Kitazawa et al.
    • Journal Title

      Toxins

      Volume: 12:146 Pages: 1-31

    • DOI

      10.3390/toxins12030146

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Immunomodulatory properties of bacterium-like particles obtained from immunobiotic lactobacilli: prospects for their use as mucosal adjuvants.2020

    • Author(s)
      Raya F, Arce L, Salva S, Alvarez S, Takahashi H, Kitazawa H, Villena J, Vizoso-Pinto, MG
    • Journal Title

      Frontiers in Immunology

      Volume: 11 Pages: 1-13

    • DOI

      10.3389/fimmu.2020.00015

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nasal priming with immunobiotic lactobacilli improves the adaptive immune response against influenza virus.2020

    • Author(s)
      Raya F, Islam MA, Vizoso-Pinto MG, Takahashi H, Kitazawa H, Villena J
    • Journal Title

      International Immunopharmacology

      Volume: 78 Pages: 106115-106115

    • DOI

      10.1016/j.intimp.2019.106115

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Draft Genome Sequence of Lactobacillus plantarum CRL681, Isolated from Argentinean Artisanal Fermented Sausages2019

    • Author(s)
      Fadda, S., J. Villena, L. Albarracin, L. Saavedra, Md. A. Islam, G. M. Vignolo, H. Kitazawa, E. M. Hebert
    • Journal Title

      Microbiol. Res. Announc.

      Volume: 8:15(e01629-18) Pages: 1-2

    • DOI

      10.1128/mra.01629-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Draft Genome Sequence of probiotic Lactobacillus brevis TUCO-5E isolated from porcine milk2018

    • Author(s)
      Quilodran-Vega, S., L., Albarracin, E., Hebert, L. Saavedra, A. Fonseca, A. S. Burgos, H, Kitazawa, J. Villena
    • Journal Title

      Microbiol. Res. Announc.

      Volume: 7:19(e01239-18) Pages: 1-2

    • DOI

      10.1128/mra.01239-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Immunododulatory properties and the genomic studies of immunobiotics and immunosynbiotic characterization2021

    • Author(s)
      周 冰卉, 犬童 優樹, Leonardo Albarracin, 舛水 由季, Aminul Islam, 大坪 和香子, 麻生 久, Julio Villena, 北澤 春樹
    • Organizer
      日本畜産学会第128回大会(九大、オンライン)
    • Related Report
      2020 Annual Research Report
  • [Presentation] Lactobacillus plantarum CRL1506における抗ウイルス性イムノジェニクスの解明2021

    • Author(s)
      舟橋 龍太郎, 水野 滉也, 友常 加恵, 友清 帝, Md. Aminul Islam, Leonardo Albarracin, 大坪 和香子, 麻生 久, Julio Villena, 北澤 春樹
    • Organizer
      日本畜産学会第128回大会(九大、オンライン)
    • Related Report
      2020 Annual Research Report
  • [Presentation] Selection of porcine immunobiotics with the ability to improve innate antiviral immunity in porcine intestinal epithelial cells2019

    • Author(s)
      Indo, Y., Md. A. Islam, H. Kitazawa et al.
    • Organizer
      LXVII REUNION CIENTIFICA ANUAL de la Sociedad Argentina de Inmunologia
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Modulation of peptidoglycan recognition proteins-mediated porcine intestinal innate immunity by paraimmunobiotic bifidobacteria2019

    • Author(s)
      Islam, Md. A., H. Iida, M. Tohno, H. Kitazawa et al.
    • Organizer
      LXVII REUNION CIENTIFICA ANUAL de la Sociedad Argentina de Inmunologia
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Anti-viral immunobiotics from porcine small intestine2019

    • Author(s)
      Indo, Y., Md. A. Islam, H. Kitazawa et al.
    • Organizer
      日本畜産学会第126回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Immunobiotic Lactobacillus jensenii TL2937 Alleviates Dextran Sodium Sulfate-Induced Colitis in Mice2019

    • Author(s)
      Islam, Md. A., V. Garcia-Castillo, N. Sato, H. Kitazawa et al.
    • Organizer
      FEMS2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Selection of wakame assimilative Lactobacilli and in vitro evaluation of their immunomodulatory potentials2019

    • Author(s)
      Zhou, B, Y. Masumizu, H. Kitazawa et al.
    • Organizer
      FEMS2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Selection of wakame utilizing lactobacilli adhering to porcine intestinal mucin and epitheliocytes2019

    • Author(s)
      Binghui Zhou, Yuki Masumizu, Yuhka Nakano, Yuhki Indo, Ryoya Komatsu, Md Aminul Islam, Wakako Ohtsubo, Yoshihito Suda, Keiichi Suzuki, Hisashi Aso, Haruki Kitazawa
    • Organizer
      日本畜産学会第125回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Construction of porcine immunobiotics library2019

    • Author(s)
      Yuhka Nakano, Yuki Masumizu, Ryusuke Ohgi, Leonardo Albarracin, Wakako Ohtsubo, Md Aminul Islam, Yoshihito Suda, Julio Villena, Tomonori Nochi, Haruki Kitazawa
    • Organizer
      日本畜産学会第125回大会
    • Related Report
      2018 Annual Research Report
  • [Book] Screening and characterization of immunobiotic lactic acid bacteria with porcine immunoassay systems. Lactic Acid Bacteria: Methods and Protocols (Ed. M. Kanauchi), pp 131-144, Meth. Mol. Biol2018

    • Author(s)
      Castillo, VG., L. Albarracin, H. Kitazawa, J. Villena
    • Total Pages
      194
    • Publisher
      Humana Press, New York, NY
    • ISBN
      9781493989072
    • Related Report
      2018 Annual Research Report
  • [Remarks] 食と農免疫国際教育研究センター

    • URL

      http://www.agri.tohoku.ac.jp/cfai/

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] JSPS研究拠点形成事業先端拠点形成「食の安全性の飛躍的向上を目指した農免疫国際共同研究拠点形成」

    • URL

      http://www.agri.tohoku.ac.jp/fain/index.html

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] 研究室HP

    • URL

      https://tohokuagri.wixsite.com/lapc

    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 抗ウイルス剤2020

    • Inventor(s)
      北澤春樹ら
    • Industrial Property Rights Holder
      北澤春樹ら
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report

URL: 

Published: 2018-10-15   Modified: 2022-12-28  

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