| Project/Area Number |
18F18085
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
白須 賢 国立研究開発法人理化学研究所, 環境資源科学研究センター, グループディレクター (20425630)
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| Co-Investigator(Kenkyū-buntansha) |
ESPINAS NINO 国立研究開発法人理化学研究所, 環境資源科学研究センター, 外国人特別研究員
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| Project Period (FY) |
2018-10-12 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2020: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2019: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2018: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Rice / Histones / Acetyltransferase / Chromatin / Plant innate immunity / Epigenetics |
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| Outline of Annual Research Achievements |
We have finished the construction of HAC701 fusion protein for rice protoplast and callus, but was never substantially tested. This will be continued as budget becomes available for this project. We have produced the hac701/wrky45 double mutant and will be ready for genotyping with the help from Plant Epigenetics Unit (OIST). This double mutant will clarify some of the reviewer’s questions on the specificity of CRISPR/Cas9 targeting used to silence HAC701. In summary, we have provided evidence for the genetic interaction of HAC701 and WRKY45. Also, we have confirmed that H3K9ac is possibly catalyzed by HAC701 acetyltransferase. Lastly, we found enrichment of WRKY45 sequences in H3K9ac fraction adding more evidence of the possible physical interaction of HAC701 with WRKY45 locus. The manuscript has been accepted for publication at Communications Biology provided major revisions be supplied.
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| Research Progress Status |
令和2年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
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