• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Role of inflammation in initiation and progression of clonal hematopoiesis

Research Project

Project/Area Number 18F18408
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Review Section Basic Section 54010:Hematology and medical oncology-related
Research InstitutionKumamoto University

Principal Investigator

滝澤 仁 (2018)  熊本大学, 国際先端医学研究機構, 特別招聘教授 (10630866)

Co-Investigator(Kenkyū-buntansha) FAKRUDDIN MD  熊本大学, 国際先端医学研究機構, 特定事業研究員 (20816475)
FAKRUDDIN MD.  熊本大学, 国際先端医学研究機構, 外国人特別研究員
Host Researcher 滝澤 仁 (2019)  熊本大学, 国際先端医学研究機構, 特別招聘教授 (10630866)
Foreign Research Fellow FAKRUDDIN MD.  熊本大学, 国際先端医学研究機構, 外国人特別研究員
Project Period (FY) 2018-10-12 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2020: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2019: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2018: ¥700,000 (Direct Cost: ¥700,000)
KeywordsClonal / Hematopoiesis / Lineage / Tracing / Leukemia / Inflammation / クローナル造血 / 炎症 / clonal hematopoiesis / inflammation
Outline of Annual Research Achievements

Many labs around the world has been working to develop novel methods for lineage tracing employing different mechanisms. But, these methods cannot provide the information on clone size of the dys-regulated and expanded clones, which led us to design this study to develop a method to determine both clonality and clone size simultaneously.
I designed, developed and evaluated the novel clonal tracing method in cell line transfected with sleeping beauty transposon vectors and analysis of data revealed that the method can detect around 500 clones (both expanded and non-expanded) with their respective clone size. Distribution of integration site analysis showed random and stochastic distribution of transposon integration in all the chromosomes. I also evaluated the method with cell number and with as low as 10000 cells, the method work well and can detect enough clones with their respective clone size. To apply this method in vivo, novel strains of mice were needed. So, I also generated two novel strains of mice- Rosa26-Hyper sleeping beauty (HSB), which contains transposase enzyme and polyIR mice, which contains multiple copy of IR transposon in silent locus of chromosome.
Upon successful evaluation of the method developed in this study in mouse model, it will provide useful tools not only in the field of hematology but also in other biomedical research fields to trace lineage to single cell resolution. The knowledge obtained from this study is expected to provide contribution towards establishment of preventive methods for pre-leukemia initiation and progression.

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (4 results)

All 2019 Other

All Int'l Joint Research (2 results) Presentation (1 results) Remarks (1 results)

  • [Int'l Joint Research] A-STAR/NUS(シンガポール)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Institute of Molecular Biology, Mainz(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Presentation] Taurine Modification of Mitochondrial tRNAs is Indispensable for Fetal Erythroid Differentiation2019

    • Author(s)
      Md. Fakruddin, Hitoshi Takizawa
    • Organizer
      第81回日本血液学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Remarks] 国際先端医学研究機構 幹細胞ストレス研究室

    • URL

      http://ircms.kumamoto-u.ac.jp/research/hitoshi_takizawa/

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report

URL: 

Published: 2018-10-15   Modified: 2024-12-25  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi