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酵素化学的ラジカルペア生成を介する抗腫瘍性発現の状態解析および遠隔磁気的制御

Research Project

Project/Area Number 18H00296
Research Category

Grant-in-Aid for Encouragement of Scientists

Allocation TypeSingle-year Grants
Review Section 2180:Nano/micro science, applied condensed matter physics, applied physics and engineering, nuclear engineering, earth resources engineering, energy engineering, biomedical engineeringand related fields
Research InstitutionTokyo Denki University

Principal Investigator

中川 秀紀  東京電機大学, 工学部, 研究員

Project Period (FY) 2018
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥530,000 (Direct Cost: ¥530,000)
Fiscal Year 2018: ¥530,000 (Direct Cost: ¥530,000)
Keywordsラジカル対機構 / ドラッグデリバリー / リポソーム
Outline of Annual Research Achievements

酵素分子は生体内化学反応機構のほぼ全てを支配し、特に金属酵素は酸化的リン酸化による電子伝達などの重要な生命過程の構成要素として深く関与している。金属酵素の磁気的性質を利用した反応制御が期待されるが、再現性の高い磁場効果の報告は殆どなされていない。対して、酵素-基質複合中間体にラジカル対形成がある場合はS-T系間交差(S-T intersystem crossing)による反応のスイッチングが可能である。ラジカル対機構を介する常磁性酵素の擬安定三重項状態における遷移状態を解析し、抗腫瘍性発現を低エネルギー磁場で量子化学的に制御することが本研究の試みである。
各種の常磁性酵素を封入した機能性のリボソーム体をポリカーボネート膜濾過法で調製し、高分解能核磁気共鳴装置(NMR)を用いた独自プログラムの分光測定によって酵素-基質複合・擬安定三重項ラジカル対の遷移状態を解析した。さらに酵素内に封入したプローブ分子の紫外可視吸収スペクトル変化からの相対量子収量を指標に電子受容性を評価した。その結果、リボソーム膜構成脂質の違いによるフリーラジカル散逸挙動に基づく反応場依存性スピン相関ラジカル対機構の最適化条件に関する重要知見を見いだした。加えて、リボソーム膜内展開型ラジカル対機構を低エネルギー磁場で制御する本システムは、ヒトが先天的に備えた生体内化学反応には殆ど影響を与えないことも明らかとなった。すなわちラジカル対機構を付与した機能性リボソームによる遠隔磁気的制御技術の新規開発には、本来の生体内機能は乱さず恒常性を維持し、リボソーム膜内のラジカル対機構のみを自在に遠隔制御しうるという一つの重要な可能性を秘めていることが分かった。本研究成果が低エネルギー磁場による極低侵襲的治療の将来的発展に貢献することが大いに期待される。

Report

(1 results)
  • 2018 Annual Research Report

Research Products

(16 results)

All 2019 2018

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (9 results)

  • [Journal Article] Application of Spin-Correlated Radical Pair System to Liposomal Drug-Delivery System under Exposure to Magnetic Fields2019

    • Author(s)
      Hidenori Nakagawa, Mikio Ohuchi, Takashi Tadokoro
    • Journal Title

      Journal of Magnetism and Magnetic Materials

      Volume: 479 Pages: 161-165

    • DOI

      10.1016/j.jmmm.2019.01.099

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photomagnetic Control of Nanoparticles with Radical Pair System : A Promising New Area of Liposomal Drug-Delivery System2019

    • Author(s)
      Hidenori Nakagawa, Mikio Ohuchi
    • Journal Title

      IEEE Transactions on Magnetics

      Volume: 55 Issue: 2

    • DOI

      10.1109/tmag.2018.2868820

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative Analysis of Unconsciousness in Extremely Low Frequency-Inducing Flickering Light Sensation2019

    • Author(s)
      Hidenori Nakagawa, Shoogo Ueno
    • Journal Title

      AIP Advances

      Volume: 9 Issue: 3

    • DOI

      10.1063/1.5080141

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Magnetohydrodynamic Approach to Effective Blood-Flow Control Utilizing Extremely Low Frequency fields2018

    • Author(s)
      Hidenori Nakagawa, Mikio Ohuchi
    • Journal Title

      IEEE Transactions on Magnetics

      Volume: 54 Issue: 11

    • DOI

      10.1109/tmag.2018.2849960

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gradient / Extremely Low Frequency Magnetic Field Affects Metamorphic Behaviors in T_4-Administrated Axolotls : Regulation of Amphibian Metamorphosis Depending on Field Strength and Exposure Timing2018

    • Author(s)
      Hidenori Nakagawa, Mikio Ohuchi
    • Journal Title

      IEEE Transactions on Magnetics

      Volume: 54 Issue: 11

    • DOI

      10.1109/tmag.2018.2845660

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Magnetohydrodynamic Approach to Effective Blood-Flow Control Utilizing ELF fields2018

    • Author(s)
      Hidenori Nakagawa, Mikio Ohuchi
    • Journal Title

      IEEE Intermag 2018 Digest Book

    • DOI

      10.1109/intmag.2018.8508699

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gradient/ELF magnetic Field Affects Metamorphic Behaviors in T_4-Administrated Axolotls : Regulation of Amphibian Metamorphosis Depending on Field Strength and Exposure Timing2018

    • Author(s)
      Hidenori Nakagawa, Mikio Ohuchi
    • Journal Title

      IEEE Intermag 2018 Digest Book

    • DOI

      10.1109/intmag.2018.8508100

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Human Reliability Measurements during Extremely Low Frequency Magnetic Stimuli2019

    • Author(s)
      Hidenori Nakagawa, Shoogo Ueno
    • Organizer
      2019 Joint MMM-Intermag Conference
    • Place of Presentation
      Washington DC (USA)
    • Year and Date
      2019-01-15
    • Related Report
      2018 Annual Research Report
  • [Presentation] Quantitative Analysis of Unconsciousness in ELF-Inducing Flickering Light Sensation2019

    • Author(s)
      Hidenori Nakagawa, Shoogo Ueno
    • Organizer
      2019 Joint MMM-Intermag Conference
    • Place of Presentation
      Washington DC (USA)
    • Year and Date
      2019-01-15
    • Related Report
      2018 Annual Research Report
  • [Presentation] Application of Spin-Correlated Radical Pair System to Liposomal DDS under Exposure to Magnetic Fields2018

    • Author(s)
      Hidenori Nakagawa, Mikio Ohuchi
    • Organizer
      9^<th> Joint European Magnetic Symposia
    • Place of Presentation
      Mainz (Germany)
    • Year and Date
      2018-09-06
    • Related Report
      2018 Annual Research Report
  • [Presentation] Photomagnetic Control of Liposomal Drug Release with Radical Pair Model2018

    • Author(s)
      Hidenori Nakagawa
    • Organizer
      9^<th> Joint European Magnetic Symposia
    • Place of Presentation
      Mainz (Germany)
    • Year and Date
      2018-09-05
    • Related Report
      2018 Annual Research Report
  • [Presentation] Extremely Low Frequency Magnetic Effects on Thyroxine-Inducing Forced Metamorphosis of Mexican Axolotl (Ambystoma mexicanum)2018

    • Author(s)
      Hidenori Nakagawa, Takashi Tadokoro, Mikio Ohuchi
    • Organizer
      9^<th> Joint European Magnetic Symposia
    • Place of Presentation
      Mainz (Germany)
    • Year and Date
      2018-09-04
    • Related Report
      2018 Annual Research Report
  • [Presentation] ELF stimulations Reveals Definite Subliminal Effects to Visual Perceptions : Human Reliability Measurements on ELF-Inducing Flickering Light Sensation2018

    • Author(s)
      Hidenori Nakagawa, Shoogo Ueno
    • Organizer
      21^<st> International Conference on Magnetism
    • Place of Presentation
      San Francisco (USA)
    • Year and Date
      2018-07-19
    • Related Report
      2018 Annual Research Report
  • [Presentation] Photomagnetic Control of Nanoparticles with Radical Pair System : A promising New Area of Liposomal DDS2018

    • Author(s)
      Hidenori Nakagawa, Mikio Ohuchi
    • Organizer
      21^<st> International Conference on Magnetism
    • Place of Presentation
      San Francisco (USA)
    • Year and Date
      2018-07-17
    • Related Report
      2018 Annual Research Report
  • [Presentation] Magnetohydrodynamic Approach to Effective Blood-Flow Control Utilizing ELF fields2018

    • Author(s)
      Hidenori Nakagawa, Mikio Ohuchi
    • Organizer
      IEEE International Magnetics Conference 2018
    • Place of Presentation
      Singapore (Singapore)
    • Year and Date
      2018-04-25
    • Related Report
      2018 Annual Research Report
  • [Presentation] Gradient/ELF magnetic Field Affects Metamorphic Behaviors in T_4-Administrated Axolotls : Regulation of Amphibian Metamorphosis Depending on Field Strength and Exposure Timing2018

    • Author(s)
      Hidenori Nakagawa, Mikio Ohuchi
    • Organizer
      IEEE International Magnetics Conference 2018
    • Place of Presentation
      Singapore (Singapore)
    • Year and Date
      2018-04-25
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2020-03-17  

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