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Development of an optimized mechanical stimulation system for cartilage tissue culture using in-situ feedback of the gene transcriptional response

Research Project

Project/Area Number 18H01398
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 20010:Mechanics and mechatronics-related
Research InstitutionDoshisha University

Principal Investigator

Yamamoto Koji  同志社大学, 生命医科学部, 教授 (70536565)

Co-Investigator(Kenkyū-buntansha) 森田 有亮  同志社大学, 生命医科学部, 教授 (80368141)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords軟骨再生 / 再生医療 / in-situフィードバック / 力学刺激 / 転写応答 / 最適化 / 培養軟骨 / 遺伝子転写応答 / in situフィードバック / メカニカルストレス / プロモーター / 力学場 / コラーゲン構造 / 組織培養 / 細胞外基質 / in-situ観察
Outline of Final Research Achievements

In regenerative medicine, it is required to develop a system enabled to culture tissue effectively in vitro. In this study, we have developed an optimized mechanical stimulation system for cartilage tissue culture using in-situ feedback of the gene transcriptional response. To reveal the relation between mechanical stimulation and production of matrix proteins of chondrocytes, we initially evaluated the effect of an orientation of collagen fibers on cellular response to mechanical stimulation in cartilage tissue. Secondly, we investigated the effect of mechanical stimulation on the formation of collagen constructs. Considering these results, we successfully optimized the mechanical stimulation adapting to cellular metabolism using in-situ monitoring of the collagen-specific gene response.

Academic Significance and Societal Importance of the Research Achievements

培養組織のタンパク質合成を促すために数多くの刺激条件探索が行われているが,装置や培養担体が変わると再度条件を再構成する実験が繰り返されている.本研究で開発したシステムでは遺伝子転写情報を利用することで,細胞の状態に適応した刺激制御を行うため,培養環境の影響が少なく効率的な刺激パターンの生成が可能となる.また,転写応答を利用しているため,力学刺激に対する分子応答が解析可能であり,新規分子経路の同定など,再生医療分野における新たな発展が期待できる.

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (12 results)

All 2020 2019 2018

All Presentation (12 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Presentation] 軟骨潤滑機能の形成促進を目指したフィードバック制御型刺激負荷培養システムの開発2020

    • Author(s)
      佐藤遼弥,森田有亮,仲町英治,山本浩司
    • Organizer
      第19回日本再生医療学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 軟骨遺伝子転写応答のin-situフィードバックによる動的力学負荷制御法の確立2020

    • Author(s)
      山本浩司,中世古拓己,森田有亮,仲町英治
    • Organizer
      第19回日本再生医療学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Mechano-responsive Ca2+ inlfux of chondrocyte increases at the boundary of cartialge zonal collagen structure2019

    • Author(s)
      Yamamoto S, Morita Y, Nakamachi E, Yamamoto K.
    • Organizer
      25th Congress of European Society of Biomechanics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In-situ analysis of time-dependent formation of cartilage collagen structure under mechanical stimulation2019

    • Author(s)
      Hamada K, Morita Y, Nakamachi E, Yamamoto K.
    • Organizer
      25th Congress of European Society of Biomechanics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In-situ evaluation of mechano-responsive Col2a1 expression of chondrocyte by using specific promoter gene2019

    • Author(s)
      Nakaseko T, Morita Y, Nakamachi E, Yamamoto K.
    • Organizer
      25th Congress of European Society of Biomechanics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In-situ evaluation for tribological maturation process of tissue-engineered cartilage cultured under shear stimulation2019

    • Author(s)
      Sato R, Nakaseko T, Morita Y, Nakamachi E, Yamamoto K.
    • Organizer
      Tissue Engineering & Regenerative Medicine International Society - AP Chapter and the 7th Asian Biomaterials Congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Generation of lubrication function on the surface of cartilage tissue2019

    • Author(s)
      Yamamoto K, Sato R, Morita Y, Nakamachi E,
    • Organizer
      The 11th International Biotribology Forum and The 40th Biotribology Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 軟骨基質遺伝子特異的プロモーターを利用した力学刺激応答のin-situ発現動態評価システムの構築2019

    • Author(s)
      山本浩司,中世古拓己,森田有亮,仲町英治
    • Organizer
      第18回日本再生医療学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 軟骨潤滑機能形成のin-situ力学・分子変動情報を利用可能な刺激負荷培養システムの開発2019

    • Author(s)
      佐藤遼弥,中世古拓己,森田有亮,仲町英治,山本浩司
    • Organizer
      日本機械学会 関西学生会2018年度学生員卒業研究発表講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 力学刺激に対する軟骨基質遺伝子の特異的プロモーターを利用したin-situ発現動態評価2018

    • Author(s)
      中世古拓己,森田有亮,仲町英治,山本浩司
    • Organizer
      第31回バイオエンジニアリング講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細胞内Ca2+変動を利用した圧縮刺激に伴う軟骨組織内刺激伝達プロセスの解析2018

    • Author(s)
      山本智史,江口剛史,森田有亮,仲町英治,山本浩司
    • Organizer
      第31回バイオエンジニアリング講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 多光子励起顕微鏡を用いた力学刺激に伴う軟骨基質コラーゲン構造形成の経時的解析2018

    • Author(s)
      濱田晃希,森田有亮,仲町英治,山本浩司
    • Organizer
      第31回バイオエンジニアリング講演会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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