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Construction of cell-electrode biointerface with self-assembling peptides

Research Project

Project/Area Number 18H01795
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionTokyo Institute of Technology

Principal Investigator

Okochi Mina  東京工業大学, 物質理工学院, 教授 (70313301)

Co-Investigator(Kenkyū-buntansha) 田中 祐圭  東京工業大学, 物質理工学院, 助教 (60533958)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywordsペプチド / 細胞界面 / 細胞計測 / ナノシート / ナノシート材料 / ナノバイオ / ナノ材料
Outline of Final Research Achievements

Two-dimensional materials are attracting attention as next-generation electronics materials that can acquire signals at high speed and with high sensitivity, and the construction of biointerfaces that do not impair material properties is a challenge. In this study, we explored peptides that exhibit affinity for cells and electronic materials in order to design biodevice interfaces using the self-assembling peptides. By simply preparing the bifunctional peptides and dropping their solution, a cell recognition interface was constructed on the surface of the specific material by self-assembly. It was suggested that this peptide interface can be used for a variety of cell types, imaging and detection, and can also be used to capture a variety of detection targets by changing the peptide sequence to suit the target object.

Academic Significance and Societal Importance of the Research Achievements

ペプチドの自己組織化により特定の材料表面に選択的に生体分子や細胞の認識界面を構築でき、電気化学的な細胞計測に利用することが可能となった。このようなエレクトロニクス材料表面における細胞認識界面の構築により、電気化学的な刺激に対する細胞応答計測も可能となることが示唆された。また、標的対象に合わせてペプチド配列を変えることにより、細菌、ウイルス、生体膜小胞をはじめ、様々な分子に対する認識界面を構築することが可能であり、センサー界面の構築法として有用であることが示唆された。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (37 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (4 results) Journal Article (11 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 11 results,  Open Access: 3 results) Presentation (22 results) (of which Int'l Joint Research: 6 results,  Invited: 3 results)

  • [Int'l Joint Research] University of Sheffield/University of Leeds(英国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Chung-Ang University(韓国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Leeds(英国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Chung-Ang University(韓国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Assemblies of bi-functional peptides on pyrolytic graphite for cell adhesion2021

    • Author(s)
      Tatematsu Soichiro、Ohnishi Tomoko、Saito Shogo、Tanaka Masayoshi、Hayamizu Yuhei、Okochi Mina
    • Journal Title

      Biochemical Engineering Journal

      Volume: 170 Pages: 107988-107988

    • DOI

      10.1016/j.bej.2021.107988

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microfluidic-based capture and release of cancer-derived exosomes via peptide?nanowire hybrid interface2021

    • Author(s)
      Suwatthanarak Thanawat、Thiodorus Ivan Adiyasa、Tanaka Masayoshi、Shimada Taisuke、Takeshita Daiki、Yasui Takao、Baba Yoshinobu、Okochi Mina
    • Journal Title

      Lab on a Chip

      Volume: 21 Issue: 3 Pages: 597-607

    • DOI

      10.1039/d0lc00899k

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of cancer-cell migration by tetraspanin CD9-binding peptide2021

    • Author(s)
      Suwatthanarak Thanawat、Tanaka Masayoshi、Miyamoto Yoshitaka、Miyado Kenji、Okochi Mina
    • Journal Title

      Chemical Communications

      Volume: 57 Issue: 40 Pages: 4906-4909

    • DOI

      10.1039/d1cc01295a

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic Exploration of Membrane Curvature Sensors Using a Series of Spherical Supported Lipid Bilayers2020

    • Author(s)
      Tanaka Masayoshi、Komikawa Takumi、Yanai Kentaro、Okochi Mina
    • Journal Title

      Analytical Chemistry

      Volume: 92 Issue: 24 Pages: 16197-16203

    • DOI

      10.1021/acs.analchem.0c04039

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Array-Based Screening of Silver Nanoparticle Mineralization Peptides2020

    • Author(s)
      Tanaka Masayoshi、Saito Shogo、Kita Reo、Jang Jaehee、Choi Yonghyun、Choi Jonghoon、Okochi Mina
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 7 Pages: 2377-2377

    • DOI

      10.3390/ijms21072377

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Peptide array-based inhibition ELISA for evaluating antigenicity in infant formulas2020

    • Author(s)
      Kubo Chisato、Kurimoto Masaki、Tanaka Masayoshi、Ochi Hiroshi、Abe Fumiaki、Okochi Mina
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 130 Issue: 4 Pages: 374-381

    • DOI

      10.1016/j.jbiosc.2020.06.010

    • NAID

      40022381075

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Systematic Screening and Deep Analysis of CoPt Binding Peptides Leads to Enhanced CoPt Nanoparticles Using Designed Peptides2020

    • Author(s)
      Jarrald Rosie M.、Liang Alvin Aw W.、Rawlings Andrea E.、Tanaka Masayoshi、Okochi Mina、Staniland Sarah S.
    • Journal Title

      Bioconjugate Chemistry

      Volume: 31 Issue: 8 Pages: 1981-1994

    • DOI

      10.1021/acs.bioconjchem.0c00348

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A bioinspired peptide matrix for the detection of 2,4,6-trinitrotoluene (TNT)2020

    • Author(s)
      Komikawa Takumi、Tanaka Masayoshi、Yanai Kentaro、Johnson Benjamin R.G.、Critchley Kevin、Onodera Takeshi、Evans Stephen D.、Toko Kiyoshi、Okochi Mina
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 153 Pages: 112030-112030

    • DOI

      10.1016/j.bios.2020.112030

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Screening and characterisation of CdTe/CdS quantum dot-binding peptides for material surface functionalisation2020

    • Author(s)
      Suwatthanarak Thanawat、Tanaka Masayoshi、Minamide Taisuke、Harvie Andrew J.、Tamang Abiral、Critchley Kevin、Evans Stephen D.、Okochi Mina
    • Journal Title

      RSC Advances

      Volume: 10 Issue: 14 Pages: 8218-8223

    • DOI

      10.1039/d0ra00460j

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Peptide-Functionalized Quantum Dots for Rapid Label-Free Sensing of 2,4,6-Trinitrotoluene2020

    • Author(s)
      Komikawa Takumi、Tanaka Masayoshi、Tamang Abiral、Evans Stephen D.、Critchley Kevin、Okochi Mina
    • Journal Title

      Bioconjugate Chemistry

      Volume: - Issue: 5 Pages: 1400-1407

    • DOI

      10.1021/acs.bioconjchem.0c00117

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Peptide Screening from a Phage Display Library for Benzaldehyde Recognition2019

    • Author(s)
      Tanaka Masayoshi、Minamide Taisuke、Takahashi Yuta、Hanai Yosuke、Yanagida Takeshi、Okochi Mina
    • Journal Title

      Chemistry Letters

      Volume: 48 Issue: 8 Pages: 978-981

    • DOI

      10.1246/cl.190318

    • NAID

      130007687695

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 単層MoS2の発光解析による破骨細胞活性の評価2021

    • Author(s)
      梅井美和子、渡邉健一、中村慶己、田中祐圭、早水裕平、大河内美奈
    • Organizer
      電気化学会第88回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ペプチドを用いた近赤外光を吸収する三角金ナノプレート合成2021

    • Author(s)
      桐木友花、田中祐圭、大河内美奈
    • Organizer
      電気化学会第88回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 脂質膜被覆球形 SiO2粒子を活用した大腸菌由来生体膜曲率認識タンパク質の探索2021

    • Author(s)
      三宅貴大、上野佑、田中祐圭、大河内美奈
    • Organizer
      電気化学会第88回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Peptide-nanowire interface for capture and release of cancer-derived exosomes2021

    • Author(s)
      スワッタナーラック タナワット、チオドル ス イワン、田中祐圭、嶋田泰佑、竹下大樹、安井隆雄、馬場嘉信、大河内美奈
    • Organizer
      電気化学会第88回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 曲率を制御した人工生体膜によるがん細胞由来の曲率認識タンパク質の網羅的探索2021

    • Author(s)
      田中 祐圭、児美川 拓実、大河内 美奈
    • Organizer
      化学工学会第86年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 単層二硫化モリブデンを用いた破骨細胞のプロトン放出計測2020

    • Author(s)
      渡邉健一、手塚沙也可、野口紘長、田中祐圭、早水裕平、大河内美奈
    • Organizer
      化学工学会第85年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Screening of CdTe quantum dot-binding peptides for quantum dot-antibody bioconjugation2019

    • Author(s)
      Thanawat Suwatthanarak, Daisuke Minamide, Masayoshi Tanaka, Andrew Harvie, Abiral Tamang, Kevin Critchley, Stephen D Evans, Mina Okochi
    • Organizer
      18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Design of peptide interface on highly oriented pyrolytic graphite substrate to observe the cytoplasmic face of cell membrane2019

    • Author(s)
      Shogo Saito, Soichiro Tatematsu, Masayoshi Tanaka, Mina Okochi
    • Organizer
      18th Asian Pacific Confederation of Chemical Engineering (APCChE)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 強固な細胞接着能を有する材料構築に向けた短鎖化ペプチド設計2019

    • Author(s)
      齊藤彰吾、立松宗一郎、Lee Hua-Yun、田中祐圭、大河内美奈
    • Organizer
      化学とマイクロ・ナノシステム学会第40回研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ペプチドアレイを反応基盤とした、ミルク抗原ペプチド検出技術の開発2019

    • Author(s)
      久保智里、栗本昌樹、田中祐圭、越智浩、阿部文明、大河内美奈
    • Organizer
      第71回 日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 光学的特性を選択可能な金ナノ粒子グリーン合成ペプチドの効率的探索2019

    • Author(s)
      田中祐圭、林美伶、高橋雄太、大河内美奈
    • Organizer
      第71回 日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 爆発性芳香族化合物の簡便・迅速な検出に向けたTNT認識ペプチド修飾量子ドットの作製2019

    • Author(s)
      児美川拓実、Abiral Tamang、田中祐圭、Kevin Critchley、Stephen Evans、大河内美奈
    • Organizer
      第71回 日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] TNT認識ペプチド修飾量子ドットを用いた爆発物の迅速検出2019

    • Author(s)
      児美川拓実、Abiral Tamang、田中祐圭、Kevin Critchley、Stephen Evans、大河内美奈
    • Organizer
      化学とマイクロ・ナノシステム学会第39回研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Mineralization peptide screening for one-pot gold nanoparticle syntheses2019

    • Author(s)
      Masayoshi Tanaka、Mina Okochi
    • Organizer
      ICPAC2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Detection of individual cells using a peptide-functionalized2019

    • Author(s)
      Mina Okochi
    • Organizer
      The Second International Workshop by the 174th Committee JSPS
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Biosensing using the functional peptide probes screened by peptide array2019

    • Author(s)
      Mina Okochi
    • Organizer
      第65回化学センサ発表会(台湾とのジョイントセッション)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 薄層グラファイト上への細胞膜結合性ペプチド界面の構築2018

    • Author(s)
      立松宗一郎、田中祐圭、大河内美奈
    • Organizer
      第12回バイオ関連化学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 高感度検出に向けたペプチド修飾ポアセンサの開発2018

    • Author(s)
      服部翔太、田中祐圭、有馬彰秀、筒井真楠、谷口正輝、大河内美奈
    • Organizer
      化学工学会第50回秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細菌結合性ペプチドの探索とZnO表面へのone-pot修飾による細菌捕捉界面の構築2018

    • Author(s)
      田中祐圭、イルファハヌンハルリサ、高橋雄太、大河内美奈
    • Organizer
      化学工学会第50回秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] TNT抗体フラグメントを用いた爆発性化合物の検出2018

    • Author(s)
      大河内 美奈、児美川 拓実、矢内 健太郎、Wang Jin、田中 祐圭、小野寺 武、都甲 潔
    • Organizer
      第12回バイオ関連化学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 複合ペプチドによる細胞ーナノ材料界面の構築2018

    • Author(s)
      齊藤彰吾、立松宗一郎、田中祐圭、大河内美奈
    • Organizer
      化学とマイクロ・ナノシステム学会第38回研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Screening of peptides binding to cancer-derived exosomes from EWI-2 protein2018

    • Author(s)
      Thanawat Suwatthanarak, Masayoshi Tanaka, Mina Okochi
    • Organizer
      The 24th Symposium of Young Asian Biological Engineers’ Community
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2023-01-30  

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