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Development of in vitro skeletal muscle tissues consisting of slow and fast fibers

Research Project

Project/Area Number 18H01796
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionNagoya University

Principal Investigator

Shimizu Kazunori  名古屋大学, 工学研究科, 准教授 (70402500)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywordsバイオマイクロシステム / 組織工学 / マイクロデバイス / 生体医用工学 / 生物工学 / 細胞アッセイ技術 / ティッシュエンジニアリング / バイオマイクロデバイス / 動物細胞培養 / BioMEMS
Outline of Final Research Achievements

In this study, we aimed to construct mosaic-like skeletal muscle tissue in vitro by taking advantage of the biological characteristic that the firing frequency of motor neurons connecting slow- and fast-twitch muscles is different, and developed the necessary technologies. First, we developed a two-dimensional co-cultured microdevice. Second, we developed a three-dimensional co-culture microdevice. Third, we found that the frequency of junction formation with co-cultured motor neurons can be improved by culturing skeletal muscle cells in an oriented manner. We plan to utilize these findings to further advance the construction of mosaic-like muscle tissue.

Academic Significance and Societal Importance of the Research Achievements

本研究では、運動神経細胞と骨格筋細胞を共培養するためのマイクロデバイスを二つ開発し、さらに効率的に共培養を行う方法を見出した。これらの知見は、運動神経細胞と骨格筋細胞に関連する疾患研究や基礎生物学研究に、広く利用することができると考えられる。また、これらを用いてモザイク状筋組織構築を実現することができれば、様々な筋関連疾患の線維タイプ選択的な発症や進行をインビトロで再現することが可能になり、筋疾患創薬研究が大きく進展すると期待される。

Report

(1 results)
  • 2021 Final Research Report ( PDF )

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

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