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A study of protein corona formation using purified carbon nanotubes

Research Project

Project/Area Number 18H01809
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 28010:Nanometer-scale chemistry-related
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Hirano Atsushi  国立研究開発法人産業技術総合研究所, 材料・化学領域, 主任研究員 (90613547)

Co-Investigator(Kenkyū-buntansha) 亀田 倫史  国立研究開発法人産業技術総合研究所, 情報・人間工学領域, 主任研究員 (40415774)
白木 賢太郎  筑波大学, 数理物質系, 教授 (90334797)
田中 丈士  国立研究開発法人産業技術総合研究所, 材料・化学領域, 上級主任研究員 (30415707)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywordsタンパク質 / カーボンナノチューブ / タンパク質コロナ / 分子動力学計算 / 分子動力学
Outline of Final Research Achievements

This study aims to clarify the mechanism of protein corona formation on carbon nanotubes. Nanomaterials including carbon nanotubes undergo protein adsorption in early stages of their uptake into biological systems, which leads to the formation of a protein layer called the protein corona. The protein corona is closely associated with various biological impacts. Thus, it is necessary to clarify the mechanism of the protein corona formation for understanding potential biotoxicity of the nanomaterials at the molecular level.

Academic Significance and Societal Importance of the Research Achievements

本研究によって、カーボンナノチューブ表面のタンパク質コロナ形成に寄与するアミノ酸残基が明らかになった。平面性の側鎖を有するアミノ酸残基(トリプトファン、チロシン、アルギニンなど)はカーボンナノチューブへの結合の起点となり、システイン残基はカーボンナノチューブおよび夾雑する遷移金属イオンによって酸化される。これらの知見はカーボンナノチューブ表面のタンパク質コロナ形成機構の理解に役立つ。今後、本知見と本手法をカーボンナノチューブ以外のナノ粒子にも適用し、タンパク質コロナ形成の包括的な予測法を確立することが、持続的な材料開発の要となる。本研究は、そのための基礎研究として、有益な知見をもたらした。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (17 results)

All 2022 2021 2020 2019 2018

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 2 results) Presentation (8 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] Aromaphilicity index: The affinity of amino acids for aromatic surfaces2022

    • Author(s)
      平野 篤、亀田 倫史
    • Journal Title

      Seibutsu-kogaku Kaishi

      Volume: 100 Issue: 1 Pages: 29-37

    • DOI

      10.34565/seibutsukogaku.100.1_29

    • NAID

      130008143997

    • ISSN
      0919-3758, 2435-8630
    • Year and Date
      2022-01-25
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Affinity of Amino Acid Side Chains for Aromatic Surfaces—Aromaphilicity: Qualitative Experiments and Quantitative Molecular Dynamics Simulations2022

    • Author(s)
      HIRANO Atsushi、KAMEDA Tomoshi
    • Journal Title

      Seibutsu Butsuri

      Volume: 62 Issue: 1 Pages: 46-49

    • DOI

      10.2142/biophys.62.46

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein’s Protein Corona: Nanoscale Size Evolution of Human Immunoglobulin G Aggregates Induced by Serum Albumin2022

    • Author(s)
      Nakayama Tomohito、Kobayashi Kaito、Kameda Tomoshi、Hase Muneaki、Hirano Atsushi
    • Journal Title

      ACS Applied Materials & Interfaces

      Volume: 14 Issue: 29 Pages: 32937-32947

    • DOI

      10.1021/acsami.2c08271

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interactions between Amino Acids and Zirconia Modified with Ethylenediaminetetra(methylenephosphonic acid): Mechanistic Insights into the Selective Binding of Antibodies2021

    • Author(s)
      Hirano Atsushi、Wada Momoyo、Kitamura Masahiro、Kasahara Shinjiro、Kato Katsuya
    • Journal Title

      Langmuir

      Volume: 37 Issue: 4 Pages: 1605-1612

    • DOI

      10.1021/acs.langmuir.0c03471

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aromaphilicity Index of Amino Acids: Molecular Dynamics Simulations of the Protein Binding Affinity for Carbon Nanomaterials2021

    • Author(s)
      Hirano Atsushi、Kameda Tomoshi
    • Journal Title

      ACS Applied Nano Materials

      Volume: 4 Issue: 3 Pages: 2486-2495

    • DOI

      10.1021/acsanm.0c03047

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The solubility of N-acetyl amino acid amides in organic acid and alcohol solutions: Mechanistic insight into structural protein solubilization2021

    • Author(s)
      Hirano Atsushi、Wada Momoyo、Sato Takehiro K.、Kameda Tomoshi
    • Journal Title

      International Journal of Biological Macromolecules

      Volume: 178 Pages: 607-615

    • DOI

      10.1016/j.ijbiomac.2021.02.141

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oxidative Stress of Carbon Nanotubes on Proteins Is Mediated by Metals Originating from the Catalyst Remains2019

    • Author(s)
      Hirano Atsushi、Wada Momoyo、Tanaka Takeshi、Kataura Hiromichi
    • Journal Title

      ACS Nano

      Volume: 13 Pages: 1805-1816

    • DOI

      10.1021/acsnano.8b07936

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of single-wall carbon nanotube redox reaction by adsorbed proteins2018

    • Author(s)
      Nakayama Tomohito、Tanaka Takeshi、Shiraki Kentaro、Hase Muneaki、Hirano Atsushi
    • Journal Title

      Applied Physics Express

      Volume: 11 Issue: 7 Pages: 075101-075101

    • DOI

      10.7567/apex.11.075101

    • NAID

      120007134059

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Carbon Nanotubes Induce Oxidative Stress in Coenzyme-Dependent Metabolic Reactions2022

    • Author(s)
      平野 篤、亀田 倫史、和田 百代、田中 丈士、片浦 弘道
    • Organizer
      第62回フラーレン・ナノチューブ・グラフェン総合シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] Size evolution of human immunoglobulin G aggregates induced by human serum albumin2021

    • Author(s)
      中山 智仁、平野 篤
    • Organizer
      第21回日本蛋白質科学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Aromaphilicity index of amino acids: Prediction of protein binding affinity for aromatic surfaces2021

    • Author(s)
      平野 篤、亀田 倫史
    • Organizer
      第59回日本生物物理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Aromaphilicity: the affinity for graphene and carbon nanotubes determined by experiments and simulations2021

    • Author(s)
      平野 篤、亀田 倫史、和田 百代、田中 丈士、片浦 弘道
    • Organizer
      第61回 フラーレン・ナノチューブ・グラフェン総合シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] Aromaphilicity index: アミノ酸と芳香族表面の相互作用の指標2021

    • Author(s)
      平野 篤、亀田 倫史
    • Organizer
      第21回日本蛋白質科学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] アミノ酸レベルから見るナノカーボンとタンパク質の相互作用原理2020

    • Author(s)
      平野 篤
    • Organizer
      溶液化学研究会若手の会オンラインシンポジウム
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Iron ion-mediated oxidation of coenzyme NADH by carbon nanotubes2020

    • Author(s)
      平野 篤、和田 百代、田中 丈士、片浦 弘道
    • Organizer
      第58回フラーレン・ナノチューブ・グラフェン総合シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] カーボンナノチューブによるタンパク質の酸化反応における触媒由来金属の効果2018

    • Author(s)
      平野 篤、和田 百代、田中 丈士、片浦 弘道
    • Organizer
      第55回 フラーレン・ナノチューブ・グラフェン 総合シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Book] 導電性材料の設計,導電性制御および最新応用展開 第6節 タンパク質を用いたカーボンナノチューブの分散技術とその原理2021

    • Author(s)
      平野篤
    • Total Pages
      6
    • Publisher
      技術情報協会
    • ISBN
      9784861048678
    • Related Report
      2021 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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